Product Information
| Protein full name | Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 |
| Synonyms | APS kinase, ATP sulfurylase, ATPSK2, PAPS synthase 2, PAPSS 2, PAPSS2, SAT Adenylyl sulfate kinase, SK 2, SK2, Sulfate adenylate transferase, Sulfurylase kinase 2 |
| Uniprot ID | O88428 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse PAPSS2 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 70 kDa / 70 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Mouse, Rat |
| Dilution: 1: 300-1: 600 | Positive Tissue: liver, kidney |
Background
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have an important role in skeletogenesis during postnatal growth.
Images
WB analysis of PAPSS2(GB113700). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).