Product Information
| Protein full name | Aldo-keto reductase family 1 member D1 |
| Synonyms | 3o5bred, AKR1D1, CBAS2, SRD5B1 |
| Uniprot ID | Q8VCX1 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse AKR1D1 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 37 kDa / 37 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Human, Mouse |
| Dilution: 1: 2000-1: 4000 | Positive Tissue: liver |
Background
Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).
Images
WB analysis of AKR1D1(GB114667). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of AKR1D1(GB114667). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).