Anti-AKR1D1 Rabbit pAb servicebio GB114667-100

$50.00
In stock
SKU
GB114667-100

 

Product Information

Protein full nameAldo-keto reductase family 1 member D1
Synonyms3o5bred, AKR1D1, CBAS2, SRD5B1
Uniprot IDQ8VCX1
ImmunogenKLH conjugated Synthetic peptide corresponding to Mouse AKR1D1
IsotypeIgG
Predicted MW./Observed MW.37 kDa / 37 kDa
PurityAffinity purification

Applications

Applications: WBSpecies: Human, Mouse
Dilution: 1: 2000-1: 4000Positive Tissue: liver

Background

Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).

Images

WB analysis of AKR1D1(GB114667). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of AKR1D1(GB114667). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

Storage

StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

 

 

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