Anti-INF2 Rabbit pAb servicebio GB115034-100

$50.00
In stock
SKU
GB115034-100

 

Product Information

Protein full nameInverted formin-2
SynonymsC14orf151, C14orf173, HBEBP2 binding protein C, Inverted formin 2, pp9484
Uniprot IDQ0GNC1
ImmunogenKLH conjugated Synthetic peptide corresponding to Mouse INF2
IsotypeIgG
Predicted MW./Observed MW.139 kDa / 170 kDa
PurityAffinity purification
Subcellular locationCytoplasm, perinuclear region

Applications

Applications: WBSpecies: Mouse
Dilution: 1: 500-1: 1000Positive Tissue: kidney, stomach, brain
Applications: IHC/IFSpecies: Human, Mouse, Rat
Dilution: 1: 500-1: 2000Positive Tissue: breast cancer, lung, lung cancer

Background

Severs actin filaments and accelerates their polymerization and depolymerization.

Images

WB analysis of INF2(GB115034). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of INF2(GB115034). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of INF2(GB115034). Sample: Human kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of INF2(GB115034). Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of INF2(GB115034). Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of INF2(GB115034). Sample: Mouse testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of INF2(GB115034). Sample: Rat kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

 

 

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