Anti-SLC40A1 Rabbit pAb servicebio GB111471-50

$50.00
In stock
SKU
GB111471-50

 

Product Information

Protein full nameSolute carrier family 40 member 1
SynonymsFerroportin-1, Iron-regulated transporter 1, Metal transporter protein 1, MTP1, Slc40a1, Fpn1, Ireg1, Slc11a3, Slc39a1
Uniprot IDQ9JHI9
ImmunogenRecombinant protein corresponding to Mouse SLC40A1
IsotypeIgG
Predicted MW./Observed MW.63 kDa / 63 kDa
PurityAffinity purification
Subcellular locationCell membrane, Cytoplasm

Applications

Applications: WBSpecies: Human, Mouse, Rat
Dilution: 1: 1000-1: 2000Positive Tissue: liver, kidney, colon
Applications: IHC/IFSpecies: Human, Mouse, Rat
Dilution: 1: 1200-1: 2400Positive Tissue: breast cancer, small intestine, gastric cancer, spleen, colon

Background

SLC40A1 is an iron-regulated transporter assumed to play roles in intestinal iron absorption and cellular iron release. It mediates iron efflux in the presence of ferroxidase. Defects in SLC40A1 cause hemochromatosis type 4 (HFE4), which is an autosomal dominant iron-loading disorder.

Images

WB analysis of SLC40A1(GB111471). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of SLC40A1(GB111471). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of SLC40A1(GB111471). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of SLC40A1(GB111471). Sample: Human breast carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of SLC40A1(GB111471). Sample: Human small intestine, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of SLC40A1(GB111471). Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of SLC40A1(GB111471). Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of SLC40A1(GB111471). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

 

 

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