Product Information
| Protein full name | Solute carrier family 40 member 1 |
| Synonyms | Ferroportin-1, Iron-regulated transporter 1, Metal transporter protein 1, MTP1, Slc40a1, Fpn1, Ireg1, Slc11a3, Slc39a1 |
| Uniprot ID | Q9JHI9 |
| Immunogen | Recombinant protein corresponding to Mouse SLC40A1 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 63 kDa / 63 kDa |
| Purity | Affinity purification |
| Subcellular location | Cell membrane, Cytoplasm |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: liver, kidney, colon |
| Applications: IHC/IF | Species: Human, Mouse, Rat |
| Dilution: 1: 1200-1: 2400 | Positive Tissue: breast cancer, small intestine, gastric cancer, spleen, colon |
Background
SLC40A1 is an iron-regulated transporter assumed to play roles in intestinal iron absorption and cellular iron release. It mediates iron efflux in the presence of ferroxidase. Defects in SLC40A1 cause hemochromatosis type 4 (HFE4), which is an autosomal dominant iron-loading disorder.
Images
WB analysis of SLC40A1(GB111471). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of SLC40A1(GB111471). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of SLC40A1(GB111471). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of SLC40A1(GB111471). Sample: Human breast carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SLC40A1(GB111471). Sample: Human small intestine, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SLC40A1(GB111471). Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SLC40A1(GB111471). Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SLC40A1(GB111471). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).