Low Melting Point Agarose servicebio GC205016-5g
$50.00
In stock
SKU
GC205016-5g
Product Information
| Product Name: Low Melting Point Agarose | Cat. No.: GC205016-5g |
| Spec.: 5 g |
Product Description/Introduction
Low Melting Point Agarose is a modified form of agarose with lower gelation and melting temperatures. Its gel melts at 65°C, remains in a fluid molten state at 37°C, and solidifies into a gel when the temperature drops below 30°C. Compared with conventional agarose, it exhibits superior molecular sieving properties and produces clearer bands, making it particularly suitable for gel electrophoresis separation and recovery of nucleic acids larger than 1000 bp, as well as for preparing culture media containing thermolabile materials. This product is used for routine nucleic acid electrophoresis, lipoprotein and immunoelectrophoresis, nucleic acid fragment recovery, in-gel reactions, tissue culture cell cloning, virus plaque assays, etc..
Storage and Shipping Conditions
Ship and store at room temperature, valid for 12 months.
Product Features
| CAS | 39346-81-1 |
| Format | White to off-white powder |
| Gel strength | ≥200 g/cm2 (1% Gel) |
| Gelling temperature | 26-30℃ (1.5% Gel) |
| Melting temperature | ≤65°C (1.5% Gel) |
| Electroosmotic value | ≤0.10 |
| Sulfate | ≤0.10% |
| Moisture | ≤10% |
| DNase | None Detected |
| RNase | None Detected |
| Protease | None Detected |
| Endonuclease/ligase inhibitors | None Detected |
Assay Protocol/Procedures
Prepare 1% Agarose Gel with 1×TBE Solution
1. The volume of the Erlenmeyer flask used for preparing the gel solution should be 2-4 times the volume of the gel solution. Add the required amount of 1×TBE to the Erlenmeyer flask. Slowly add agarose to the buffer while stirring continuously to prevent agarose aggregation. Record the total weight of the flask and the solution.
2. Heat the mixture on high power in a microwave for 30 seconds, adjust the heating time according to the volume of the solution. The heating duration is related to the microwave power, flask size, and agarose concentration.
3. Shake the agarose solution to mix thoroughly.
4. Heat again on high power for 30 seconds and shake the agarose solution well.
5. Place the solution back into the microwave and heat on high power until it boils (approximately 10-35 seconds). The agarose gel solution may boil violently when touched or moved; handle with caution to avoid scalding. After removing from the microwave, cool it at room temperature for 1-2 minutes and gently swirl to release air bubbles in the liquid.
6. Return the solution to the microwave and heat on high power to boil for about 15 seconds. Assess the dissolution state of the agarose. If particles still exist, repeat this step until all crystals are completely dissolved.
7. Once the agarose is fully dissolved into a clear liquid, weigh the total mass again, calculate the volume of evaporated liquid, replenish with distilled water to restore the original weight, and shake the liquid to homogenize.
8. It is recommended to pour the gel solution when it cools to 50-55℃, which helps ensure uniform gel pore size and prevents damage to the gel casting apparatus. Before pouring, gently swirl the agarose solution to release residual air bubbles.
9. Pour the gel solution into the gel casting tray; the typical gel thickness is 3-5 mm. After pouring, try to remove any air bubbles between or beneath the comb teeth.
10. Allow the gel to completely solidify at room temperaturefor 30-45 minutes.
Note
1. The buffer used for preparing the gel must be completely consistent with that used for electrophoresis.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
For Research Use Only!
Ver. No.: V1.0-20251204
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