SerBlue Nucleic Acid Dye (10,000×, Water Soluble) servicebio G3072-5ML
$50.00
In stock
SKU
G3072-5ML
Product Information
| Product Name: SerBlue Nucleic Acid Dye (10,000×, Water Soluble) | Cat. No.: G3072-100UL |
| Spec.: 100 µL | G3072-500UL |
| 500 µL | G3072-5ML |
| 5×1 mL |
Product Description/Introduction
SerBlue has a unique molecular structure and is a highly sensitive, non-mutagenic, ultra-safe and ultra-stable fluorescent nucleic acid gel staining reagent for the detection of ssDNA, RNA and dsDNA in agarose gel, which can replace unsafe nucleic acid dyes such as ethidium bromide (EtBr, EB) with much higher sensitivity than EB and lower background signal. There is no need for decolorization, even if the amount of DNA samples is very high, the band separation effect can be obtained. The dye can be excited by a 488 nm laser and directly observed with a blue light Gel cutter or a blue light scanner.
Storage and Shipping Conditions
Ship and store at room temperature away from light; valid for 24 months.
Product Content
| Component: SerBlue Nucleic Acid Dye (10,000×, Water Soluble) | G3072-100UL: 100 µL |
| G3072-500UL: 500 µL | G3072-5ML: 5×1 mL |
| Manual | One copy |
Assay Protocol/Procedures
1. Gel dyeing method
1) To prepare the gel, add 5 µL of SerBlue Nucleic Acid Dye (10,000 x, Water Soluble) per 50 mL of agarose (GC205013 recommended) gel (SerBlue has excellent thermal stability and can be added directly to the high-temperature gel solution without waiting for the gel solution to cool. It can also be made by pre-mixing SerBlue with an electrophoresis buffer containing agarose powder and heating it), and mix thoroughly, then poured, and wait for agarose gel to completely solidify for sample electrophoresis.
2) Electrophoresis according to conventional methods, followed by blue light imaging.
2. Soak dyeing method
1) Electrophoresis according to conventional methods.
2) Dilute SerBlue nucleic acid dye (10,000×, water soluble) 3,300 times with 1×TAE or TBE to make 3×staining solution (for example, to prepare 50 mL of dye solution, add 15 µL SerBlue nucleic acid dye (10,000×, water-soluble) to 50 mL of 1×TAE or TBE and mix well for later use).
3) Carefully place the gel into a suitable container and soak the gel with a sufficient amount of 3×staining solution, and incubate it for 30 min in a shaker at room temperature away from light. If it is acrylamide gel, incubated for 30 min to 1 h, and extended with the increase of acrylamide content.
4) After staining, blue light imaging.
Note
1. Immediately centrifuge the product to the bottom of the tube before use, and then conduct subsequent experiments.
2. The dye does not need to be refrigerated at low temperature. If precipitation is found, please heat the dye to 45-50℃ for 2 min, shake it to dissolve, and it will not affect the use effect.
3. The gel prepared by the gel dyeing method is light orange red. After electrophoresis, the gel color may be uneven when observed by the naked eye (the upper part of the gel color is dark, the lower part of the gel color is light), which is a normal phenomenon and does not affect the electrophoresis result.
4. SerBlue is suitable for blue light Gel cutting machines and blue light scanners, and can also be used in ultraviolet gel imaging systems, but the ultraviolet imaging bands are darker.
5. The product can stain single-stranded DNA and RNA, but is less sensitive to single-stranded DNA or RNA than double-stranded DNA.
6. The dyeing solution can be reused for about 3 times. It is recommended to store the dyeing solution away from light.
7. It is recommended that operators use it in specific areas, take protective measures, wear gloves and masks, and properly dispose of waste.
For Research Use Only!
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