Chloramphenicol servicebio GC301018-5g
$50.00
In stock
SKU
GC301018-5g
Product Information
| Product Name | Cat. No. |
| Spec. | Chloramphenicol |
| GC301018-5g | 5 g |
Description/ Introduction
Chloramphenicol, a bacterial broad-spectrum antibiotic, inhibits the growth of Gram-negative and Gram-positive bacteria by inhibiting mRNA translation on the 50S ribosome during peptide bond formation catalyzed by peptide bond transferase. In addition, chloramphenicol also has rickettsiae resistance and psittace-lymphogranulomatous chlamydia resistance, so chloramphenicol can be used for bacterial screening. Acetylation of the chloramphenicol acetyltransferase gene (CAT gene) resists chloramphenicol resistance. Based on this principle, chloramphenicol can be used as a screening drug to screen transformed cells containing chloramphenicol resistance gene in molecular biology experiments. The recommended effective working concentration is 5-20 µg/ mL.
Storage and Handling Conditions
Store at 2-8℃, valid for 48 months.
Basic attributes
| English name | Chloramphenicol |
| Synonyms | Levomycin, levomycin |
| CAS | 56-75-7 |
| Molecular formula | C11H12Cl2N2O5 |
| Molecular weight | 323.13 |
| Purity | ≥99% |
| Appearance (character) | Light yellow crystalline fine powder |
| Storage condition | 2-8℃ |
| Units | bottle |
| Model | 5 g |
| Pubchem CID | 5959 |
| MDL number | MFCD00078159 |
| EC number | 200-287-4 |
| Category | Biochemical reagents, antibiotics |
| Solubility | Solubility 50 mg/ ml in Ethanol |
Assay Protocol / Procedures
The usage and dosage of biochemical reagents are mainly based on the customer's experimental purpose and the experimental methods in the existing literature or books, and the following uses are for reference only.
Solubility: Soluble in methanol, ethanol, butanol, acetone and ethyl acetate, slightly soluble in ether, insoluble in benzene and petroleum ether.
Preparation of storage solution: 1g chloramphenicol was dissolved in 20mL 100% ethanol and fully dissolved to obtain 50 mg/ mL mother liquor. After filtration and sterilization with 0.22 µM organic phase filtration membrane, it was divided into small quantities and stored at -20°C to avoid repeated freeze-thaw, effective for one year.
[Note]: Chloramphenicol storage solution cannot be autoclaved.
Note:
1. For your safety and health, please wear lab coat and disposable gloves during operation
Security Information:
| Warning Statement | P201,P308+P313, |
| RTECS | AB6825000 |
| WGK | 3 |
For Research Use Only!
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