Anti-PHAPI2/APRIL Rabbit pAb servicebio GB112911-50
$50.00
In stock
SKU
GB112911-50
Product Information
| Protein full name | Acidic leucine-rich nuclear phosphoprotein 32 family member B |
| Synonyms | Proliferation-related acidic leucine-rich protein PAL31, Anp32b, Pal31, PAL 31, Ssp29, PHAPI2a |
| Uniprot ID | Q9EST5 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse PHAPI2 / APRIL |
| Isotype | IgG |
| Predicted MW./Observed MW. | 31 kDa / 31 kDa |
| Purity | Affinity purification |
| Subcellular location | Nucleus |
Applications
| Applications: WB | Species: Human |
| Dilution: 1: 500-1: 1000 | Positive Tissue: Jurkat |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 300-1: 1200 | Positive Tissue: spleen, heart, kidney |
Background
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
Images
WB analysis of PHAPI2/APRIL(GB112911). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IF analysis of PHAPI2/APRIL(GB112911)red. Sample: Rat prostate gland, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of PHAPI2/APRIL(GB112911)red. Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
