Anti-Nebulette Rabbit pAb servicebio GB113046-50
$50.00
In stock
SKU
GB113046-50
Product Information
| Protein full name | LIM zinc-binding domain-containing Nebulette |
| Synonyms | Actin-binding Z-disk protein, Nebl, nebl, LNEBL, LIM nebulette |
| Uniprot ID | Q0II04 |
| Immunogen | Recombinant protein corresponding to Mouse Nebulette |
| Isotype | IgG |
| Predicted MW./Observed MW. | 31 kDa / 31/130 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
Applications
| Applications: WB | Species: Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: heart, liver |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 500-1: 1000 | Positive Tissue: heart |
Background
Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion.
Images
WB analysis of Nebulette(GB113046). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of Nebulette(GB113046). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of Nebulette(GB113046). Sample: Mouse heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
