| Catalog No. | Size | Price (USD) |
| 80106-T52-50 | 50 µL | $147.96 |
| 80106-T52-100 | 100 µL | $251.64 |
| 80106-T52-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Ephrin-B1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Rat |
| Specificity | Rat Ephrin-B1 |
| Immunogen | Recombinant Rat Ephrin-B1 / EFNB1 protein (Catalog#80106-R08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Rat Ephrin-B1 / EFNB1 (Catalog#80106-R08H; P52796; Met1-Thr229). Ephrin-B1 / EFNB1 specific IgG was purified by Rat Ephrin-B1 / EFNB1 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-EFL-3 Antibody; Anti-Efnb1 Antibody; Anti-Eplg2 Antibody; Anti-LERK2 Antibody
Image / Experimental Information
| Image 1 Description | rat EFNB1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate 2 µL anti-rat EFNB1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-rat EFNB1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 33 kDa Observed band size: 33 kDa |
| Image 1 Alt Text | Rat Ephrin-B1/EFNB1 Immunoprecipitation(IP) 8272 |
| Image 1 URL | Source image |
| Image 2 Description | rat EFNB1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate 2 µL anti-rat EFNB1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-rat EFNB1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 33 kDa Observed band size: 33 kDa |
| Image 2 Alt Text | Rat Ephrin-B1/EFNB1 Western blot (WB) 13741 |
| Image 2 URL | Source image |
Background Information
| Full Name | ephrin-B1 |
| Description | Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays. |
| References |
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