| Catalog No. | Size | Price (USD) |
| 50134-R766-20 | 20 µL | $118.49 |
| 50134-R766-50 | 50 µL | $177.58 |
| 50134-R766-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-CD4 Antibody, Rabbit Monoclonal |
| Validated applications | IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse CD4 |
| Immunogen | Recombinant Mouse CD4 Protein (Catalog#50134-M08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD4 (rM CD4; Catalog#50134-M08H; NP_038516.1; Met1-Thr394). |
| Source | Monoclonal Rabbit IgG Clone #766 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Cd4 Antibody; Anti-L3T4 Antibody; Anti-Ly-4 Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of mouse CD4 in mouse spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. |
| Image 1 Alt Text | Mouse CD4 Immunohistochemistry(IHC) 22683 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of mouse CD4 in mouse spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. |
| Image 2 Alt Text | Mouse CD4 Immunohistochemistry(IHC) 22684 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of mouse CD4 in mouse spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. |
| Image 3 Alt Text | Mouse CD4 Immunohistochemistry(IHC) 22685 |
| Image 3 URL | Source image |
Background Information
| Full Name | CD4 molecule |
| Description | T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4. |
| Related Pathways |
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| References |
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title: HMG Box-containing Protein 1 (HBP1) Protects Against Pancreatic Injury in Acute Pancreatitis but Promotes Neoplastic Progression
authors: Seung-Won Lee; Taelor Ekstrom; ...; Jungsun Kim
journal: Cellular and molecular gastroenterology and hepatology
date: 2025-01-01
pmid: 40404099
doi: 10.1016/j.jcmgh.2025.101536
cited_by_count: 1
techniques: Activation Assay; Blocking Assay; Expressing; Immunohistochemistry; Immunostaining; Incubation; Marker
bioz_score: 93
title: Expression of the membrane tetraspanin claudin 18 on cancer cells promotes T lymphocyte infiltration and antitumor immunity in pancreatic cancer.
authors: Francesco De Sanctis; Silvia Dusi; ...; Vincenzo Bronte
journal: Immunity
date: 2024-06-12
pmid: 38749447
doi: 10.1016/j.immuni.2024.04.021
cited_by_count: 17
techniques: Amplex Red Cholesterol Assay; Bicinchoninic Acid Protein Assay; Control; Enzyme-linked Immunosorbent Assay; Extraction; Infection; Labeling; Lysis; Membrane; Microarray; Phospholipid Assay; Plasmid Preparation; Protease Inhibitor; Purification; Recombinant; Reverse Transcription; SYBR Green Assay; Staining; Transfection; Virus; cDNA Synthesis
bioz_score: 93
title: Preclinical characterization and phase 1 results of ADG106 in patients with advanced solid tumors and non-Hodgkin's lymphoma.
authors: Yuxiang Ma; Fan Luo; ...; Hongyun Zhao
journal: Cell Reports Medicine
date: 2024-02-23
pmid: 38330942
doi: 10.1016/j.xcrm.2024.101414
cited_by_count: 11
techniques: ATP Bioluminescent Assay; Activation Assay; Activity Assay; Bioprocessing; Cell Culture; Concentration Assay; Control; Enzyme-linked Immunosorbent Assay; Expressing; Histopathology; Immunohistochemistry; Incubation; Isolation; Luciferase; Staining
bioz_score: 93
title: Anti-tumor activity of intratumoral xenogeneic urothelial cell monotherapy or in combination with chemotherapy in syngeneic murine models of bladder cancer
authors: Chi-Ping Huang; Hsin-Ling Lu; Chih-Rong Shyr
journal: American journal of cancer research
date: 2023-06-15
pmid: 37424801
pmcid: 10326576
cited_by_count: 5
techniques: Cytometry; Enzyme-linked Immunosorbent Assay; Fluorescence; Immunohistochemistry; Incubation; Isolation; Light Microscopy; Quantitation Assay; Software; Staining
bioz_score: 93
title: Intratumoral xenogeneic tissue-specific cell immunotherapy inhibits tumor growth by increasing antitumor immunity in murine triple negative breast and pancreatic tumor models.
authors: Chi-Ping Huang; Liang-Chih Liu; ...; Chih-Rong Shyr
journal: Cancer letters
date: 2022-08-16
pmid: 35902043
doi: 10.1016/j.canlet.2021.10.044
cited_by_count: 11
techniques: Control; Immunohistochemistry; Incubation; Light Microscopy; Staining
bioz_score: 93
title: Single-dose immunisation with a multimerised SARS-CoV-2 receptor binding domain (RBD) induces an enhanced and protective response in mice.
authors: Ralf Salzer; Jordan J Clark; ...; Jan Löwe
journal: FEBS letters
date: 2021-10-13
pmid: 34331769
cited_by_count: 24
techniques: Blocking Assay; Incubation
bioz_score: 93
