| Catalog No. | Size | Price (USD) |
| KIT10947A-1 | 1 Kit (96 Tests) | $414.71 |
| KIT10947A-5 | 5 Kit (96 Tests) | $1,747.71 |
| KIT10947A-48 | 48 Tests | $266.60 |
General Information
| Product name | Human IL-10 ELISA Kit |
| Specificity | This assay recognizes both recombinant and natural Human IL10. |
| Conjugate | HRP |
| Shipping | This ELISA Kit is shipped at ambient temperature. |
| Storage | Unopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA |
| Assay type | Solid Phase Sandwich ELISA (quantitative) |
| Sample type | Cell supernatant |
| Sensitivity | 5.84 pg/mL |
| Assay range | 15.63-1000 pg/mL |
| Materials provided | 1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution |
| Product overview | This Human IL-10 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 protein in Cell supernatant . It contains recombinant Human IL-10, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use. |
| Interference | Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL10 control were assayed for interference. No significant interference was observed. |
Synonyms: CSIF ELISA Kit, Human; GVHDS ELISA Kit, Human; IL-10 ELISA Kit, Human; IL10A ELISA Kit, Human; TGIF ELISA Kit, Human
Image / Experimental Information
| Image 1 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 1 Alt Text | Human IL10/IL-10/Interleukin-10 ELISA Kit Standard Curve 15933 |
| Image 1 URL | Source image |
| Image 2 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 2 Alt Text | Human IL10/IL-10/Interleukin-10 ELISA Kit Specificity 15942 |
| Image 2 URL | Source image |
Background Information
| Full Name | interleukin 10 |
| Description | IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. |
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