| Catalog No. | Size | Price (USD) |
| KIT10602-1 | 1 Kit (96 Tests) | $414.71 |
| KIT10602-5 | 5 Kit (96 Tests) | $1,747.71 |
| KIT10602-48 | 48 Tests | $266.60 |
General Information
| Product name | Human TNF-alpha ELISA Kit |
| Specificity | This assay recognizes both recombinant and natural Human TNFa. |
| Conjugate | HRP |
| Shipping | This ELISA Kit is shipped at ambient temperature. |
| Storage | Unopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA |
| Assay type | Solid Phase Sandwich ELISA (quantitative) |
| Sample type | Cell supernatant |
| Sensitivity | 7.03 pg/mL |
| Assay range | 31.25-2000 pg/mL |
| Materials provided | 1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution |
| Product overview | This Human TNF-alpha ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF-alpha protein in Cell supernatant . It contains recombinant Human TNF-alpha, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use. |
| Interference | Preparations of the following factors at 50 ng/mL in a mid-range recombinant Human TNFa control were assayed for interference. No significant interference was observed. |
Synonyms: DIF ELISA Kit, Human; TNF-alpha ELISA Kit, Human; TNFA ELISA Kit, Human; TNFSF2 ELISA Kit, Human; TNLG1F ELISA Kit, Human
Image / Experimental Information
| Image 1 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 1 Alt Text | Human TNF-alpha/TNFA/TNFSF2 ELISA Kit Standard Curve 15932 |
| Image 1 URL | Source image |
| Image 2 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 2 Alt Text | Human TNF-alpha/TNFA/TNFSF2 ELISA Kit Specificity 15941 |
| Image 2 URL | Source image |
Background Information
| Full Name | tumor necrosis factor |
| Description | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. |
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