| Catalog No. | Size | Price (USD) |
| 10108-MM36-20 | 20 µg | $236.98 |
| 10108-MM36-50 | 50 µL | $133.15 |
| 10108-MM36-100 | 100 µg | $592.44 |
| 10108-MM36-200 | 200 µL | $340.51 |
General Information
| Product name | Recombinant Anti-ACE2 Neutralizing Antibody |
| Validated applications | ELISA (FAQ Protocol), IHC-P (FAQ Protocol), FCM (FAQ Protocol), Neutralization |
| Species reactivity | Reacts with: Human |
| Specificity | Human ACE2 |
| Immunogen | Recombinant Human ACE2 Protein (Catalog#10108-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACE2 (rh ACE2; Catalog #10108-H08H; Met1-Ser740; NP_068576.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #MM36H |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Endotoxin | < 3 EU/mg |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ACEH Antibody; Anti-UNQ868/PRO1885 Antibody
Image / Experimental Information
| Image 1 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on (ACE2 & TMPRSS2) overexpressioned 293T cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-ACE2 Neutralizing Antibody (Catalog# 10108-MM36). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 is typically 5-20 μg/mL. |
| Image 1 Alt Text | Human ACE2 Microneutralizaiton (MN) 30749 |
| Image 1 URL | Source image |
| Image 2 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on (ACE2 & TMPRSS2) overexpressioned 293T cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-ACE2 Neutralizing Antibody (Catalog# 10108-MM36). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 is typically 5-20 μg/mL. |
| Image 2 Alt Text | Human ACE2 Enzyme linked immunosorbent assay (ELISA) 30752 |
| Image 2 URL | Source image |
| Image 3 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on (ACE2 & TMPRSS2) overexpressioned 293T cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-ACE2 Neutralizing Antibody (Catalog# 10108-MM36). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 is typically 5-20 μg/mL. |
| Image 3 Alt Text | Human ACE2 Immunohistochemistry(IHC) 30755 |
| Image 3 URL | Source image |
| Image 4 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on (ACE2 & TMPRSS2) overexpressioned 293T cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-ACE2 Neutralizing Antibody (Catalog# 10108-MM36). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 is typically 5-20 μg/mL. |
| Image 4 Alt Text | Human ACE2 Immunohistochemistry(IHC) 30757 |
| Image 4 URL | Source image |
| Image 5 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on (ACE2 & TMPRSS2) overexpressioned 293T cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-ACE2 Neutralizing Antibody (Catalog# 10108-MM36). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 is typically 5-20 μg/mL. |
| Image 5 Alt Text | Human ACE2 Flow Cytometry (FC) 30759 |
| Image 5 URL | Source image |
Background Information
| Full Name | angiotensin I converting enzyme 2 |
| Description | Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis. |
| References |
|
title: Comprehensive N-glycosylation profiling of recombinant spike S1 protein from the wild-type SARS-CoV-2 and its variants
authors: Yao Peng; Tian-Tian Tong; ...; Jing-Rong Wang
journal: Frontiers in Immunology
date: 2025-07-16
doi: 10.3389/fimmu.2025.1592142
cited_by_count: 0
techniques: Binding Assay; Comparison; Concentration Assay; Incubation; Kinetic Assay; Recombinant
bioz_score: 93
title: Host cell lectins ASGR1 and DC-SIGN jointly with TMEM106B confer ACE2 independence and imdevimab resistance to SARS-CoV-2 pseudovirus with spike mutation E484D
authors: Prerna Arora; Lu Zhang; ...; Kanta Subbarao
journal: Journal of virology
date: 2025-02-01
pmid: 39791910
doi: 10.1128/jvi.01230-24
pmcid: 11852847
cited_by_count: 3
techniques: Activity Assay; Binding Assay; Control; Expressing; Flow Cytometry; Incubation; Inhibition; Luciferase; Mutagenesis; Plasmid Preparation; Protein Binding; Transduction; Transfection; Two Tailed Test; Western Blot
bioz_score: 93
title: ACE2-independent sarbecovirus cell entry can be supported by TMPRSS2-related enzymes and can reduce sensitivity to antibody-mediated neutralization
authors: Lu Zhang; Hsiu-Hsin Cheng; ...; Christopher F. Basler
journal: PLoS Pathogens
date: 2024-11-13
pmid: 39536058
doi: 10.1371/journal.ppat.1012653
pmcid: 11559990
cited_by_count: 9
techniques: Activity Assay; Binding Assay; Blocking Assay; Control; Derivative Assay; Expressing; Flow Cytometry; Fluorescence; Incubation; Knock-Out; Luciferase; MANN-WHITNEY; Neutralization; Plasmid Preparation; Recombinant; Transduction; Transfection; Two Tailed Test; Virus; Western Blot
bioz_score: 93
title: Virological Traits of the SARS-CoV-2 BA.2.87.1 Lineage
authors: Lu Zhang; Alexandra Dopfer-Jablonka; ...; Ralph A. Tripp
journal: Vaccines
date: 2024-05-01
doi: 10.3390/vaccines12050487
pmcid: 11125805
cited_by_count: 2
techniques: Activity Assay; Binding Assay; Expressing; Incubation; Luciferase; Two Tailed Test; Virus
bioz_score: 93
title: ACE2-independent sarbecovirus cell entry is supported by TMPRSS2-related enzymes and reduces sensitivity to antibody-mediated neutralization
authors: Zhang Lu; Cheng Hsiu-Hsin; ...; Hoffmann Markus
journal: bioRxiv
date: 2024-04-18
doi: 10.1101/2024.04.18.590061
cited_by_count: 1
techniques: Activity Assay; Binding Assay; Blocking Assay; Derivative Assay; Expressing; Flow Cytometry; Incubation; Luciferase; Neutralization; Plasmid Preparation; Recombinant; Transduction; Transfection; Two Tailed Test; Virus; Western Blot
bioz_score: 93
title: SARS-CoV-2 BA.2.86 enters lung cells and evades neutralizing antibodies with high efficiency.
authors: Lu Zhang; Amy Kempf; ...; Stefan Pöhlmann
journal: Cell
date: 2024-01-05
pmid: 38194966
cited_by_count: 68
techniques: Affinity Purification; Clinical Proteomics; Produced; Recombinant; Virus
bioz_score: 93
title: Discovery of a Natural Product with Potent Efficacy Against SARS-CoV-2 by Drug Screening
authors: Daixi Li; Cheng Wang; ...; Dongqing Wei
journal: Interdisciplinary sciences, computational life sciences
date: 2021-09-12
pmid: 34510373
doi: 10.1007/s12539-021-00477-w
pmcid: 8435140
cited_by_count: 6
techniques: Binding Assay; Blocking Assay; Cell Culture; Immunofluorescence; Incubation; Infection; Inhibition; Microscopy; Modification; Staining; Standard Deviation; Sterility
bioz_score: 93
title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection
authors: Cheng Wang; Shaobo Wang; ...; Jinghong Zhao
journal: ACS Nano
date: 2021-03-18
pmid: 33734675
doi: 10.1021/acsnano.0c06836
pmcid: 8009101
cited_by_count: 60
techniques: Activity Assay; Binding Assay; Centrifugation; Enzyme-linked Immunosorbent Assay; Expressing; FLAG-tag; Flow Cytometry; Fluorescence; Imaging; Immunofluorescence; In Vitro; Incubation; Infection; Injection; Labeling; Microscopy; Staining; Transmission Electron Microscopy; Western Blot
bioz_score: 93
title: IgM autoantibodies recognizing ACE2 are associated with severe COVID-19
authors: Casciola-Rosen Livia; Thiemann David R.; ...; Rosen Antony
journal: medRxiv
date: 2020-10-15
cited_by_count: 30
techniques: Activation Assay; Activity Assay; Binding Assay; Enzyme-linked Immunosorbent Assay; Incubation; MANN-WHITNEY; Negative Control; Positive Control; Purification; Quantitation Assay; Recombinant; Western Blot
bioz_score: 93
