| Catalog No. | Size | Price (USD) |
| 10980-MM48-F-25 | 25 Tests | $22.22 |
| 10980-MM48-F-100 | 100 Tests | $44.43 |
General Information
| Product name | Anti-CD8 alpha Antibody (FITC), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD8 alpha |
| Immunogen | Recombinant Human CD8 alpha/CD8A Protein (Catalog#10980-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD8 alpha/CD8A (rh CD8 alpha/CD8A; Catalog#10980-H08H; NP_001759.3; Met1-Asp182) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| Source | Monoclonal Mouse IgG2b Clone #48 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Concentration | 5 μl/Test, 0.1 mg/ml |
| Conjugate | FITC |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! |
Synonyms: Anti-CD8 Antibody; Anti-CD8alpha Antibody; Anti-IMD116 Antibody; Anti-Leu2 Antibody; Anti-MAL Antibody; Anti-p32 Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of Human CD8a expression on human whole blood lymphocyte. Cells were stained with FITC-conjugated anti-Human CD8a. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Image 1 Alt Text | Human CD8 alpha Flow Cytometry (FC) 21123 |
| Image 1 URL | Source image |
Background Information
| Full Name | CD8a molecule |
| Description | T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. |
