| Catalog No. | Size | Price (USD) |
| 40859-V08H1-100 | 100 µg | $669.46 |
General Information
| Formulation | Lyophilized from sterile 25mM Sodium Citrate, 200mM NaCl, 0.02% Tween 80, pH 6.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90% as determined by SEC-MALS. |
| Activity | Immobilized Influenza A H3N2 (A/Darwin/9/2021) Hemagglutinin / HA trimer Protein (His Tag)(HPLC-verified) (Cat:40859-V08H1) at 1μg/mL (100μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody (CR8020), the EC 50 is 2.5ng/mL-7ng/mL. |
| Protein Construction | A DNA sequence encoding the Influenza A virus ((A/Darwin/9/2021) (H3N2)) hemagglutinin (translated amino acids of EPI1859996), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. |
| Expressed Host | HEK293 Cells |
| Species | H3N2 |
| Predicted N Terminal | Gln 17 |
| Molecule Mass | The recombinant Influenza A virus ((A/Darwin/9/2021) (H3N2))Hemagglutinin / HA comprises 558 amino acids and has a predicted molecular mass of 62.34 kDa. It migrates as an approximately 87.6 kDa band in SDS-PAGE under reducing conditions. |
Image / Experimental Information
| Image 1 Description | Immobilized Influenza A H3N2 (A/Darwin/9/2021) Hemagglutinin / HA trimer Protein (His Tag)(HPLC-verified) (Cat:40859-V08H1) at 1μg/mL (100μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody (CR8020), the EC 50 is 2.5ng/mL-7ng/mL. |
| Image 1 Alt Text | H3N2 Hemagglutinin/HA Protein 37221 |
| Image 1 URL | Source image |
| Image 2 Description | Immobilized Influenza A H3N2 (A/Darwin/9/2021) Hemagglutinin / HA trimer Protein (His Tag)(HPLC-verified) (Cat:40859-V08H1) at 1μg/mL (100μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody (CR8020), the EC 50 is 2.5ng/mL-7ng/mL. |
| Image 2 Alt Text | H3N2 Hemagglutinin/HA Protein 37168 |
| Image 2 URL | Source image |
| Image 3 Description | Immobilized Influenza A H3N2 (A/Darwin/9/2021) Hemagglutinin / HA trimer Protein (His Tag)(HPLC-verified) (Cat:40859-V08H1) at 1μg/mL (100μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody (CR8020), the EC 50 is 2.5ng/mL-7ng/mL. |
| Image 3 Alt Text | H3N2 Hemagglutinin/HA Protein 37117 |
| Image 3 URL | Source image |
Background Information
| Full Name | Hemagglutinin |
| Description | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| References |
|
title: mRNA-based influenza vaccine expands the B cell response breadth in humans
authors: Hanover C. Matz; Tae-Geun Yu; ...; Ali H. Ellebedy
journal: Nature immunology
date: 2026-06-15
pmid: 42297975
doi: 10.1038/s41590-026-02569-5
cited_by_count: 3
techniques: Binding Assay; Blocking Assay; Clinical Proteomics; Enzyme-linked Immunosorbent Assay; Enzyme-linked Immunospot; Flow Cytometry; Incubation; MANN-WHITNEY; Recombinant; Two Tailed Test
bioz_score: 95
title: Exogenous melatonin boosts vaccine-induced immunity in individuals with high pre-existing influenza immunity
authors: Kyosuke Oda; Janine Danko; ...; Wathsala Wijayalath
journal: Frontiers in Immunology
date: 2025-10-24
doi: 10.3389/fimmu.2025.1663763
cited_by_count: 0
techniques: Produced; Recombinant
bioz_score: 95
title: Conserved sites on the influenza H1 and H3 hemagglutinin recognized by human antibodies
authors: Daniel P. Maurer; Mya Vu; ...; Aaron G. Schmidt
journal: Science Advances
date: 2025-04-25
pmid: 40267182
doi: 10.1126/sciadv.adu9140
pmcid: 12017299
cited_by_count: 5
techniques: Cell Isolation; FACS; Fluorescence; Incubation; Isolation; Saline; Staining
bioz_score: 95
title: mRNA-based influenza vaccine expands breadth of B cell response in humans
authors: Matz Hanover C.; Yu Tae-Geun; ...; Ellebedy Ali H.
journal: bioRxiv
date: 2024-10-13
cited_by_count: 0
techniques: Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Incubation; Recombinant
bioz_score: 95
title: An S1 subunit vaccine and combination adjuvant (COVAC-1) elicits robust protection against SARS-CoV-2 challenge in African green monkeys
authors: Garnett Lauren; Tran Kaylie N; ...; Falzarano Darryl
journal: bioRxiv
date: 2022-06-17
doi: 10.1101/2022.06.16.496375
cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay; Recombinant
bioz_score: 95
