| Catalog No. | Size | Price (USD) |
| 10279-H08H-UE-1 | 1 mg | $1,645.51 |
| 10279-H08H-UE-100 | 100 µg | $253.27 |
General Information
| Formulation | Supplied as sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 0.5 EU per mg protein. |
| Shipping | It is supplied and shipped as liquid with dry ice. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Purity | ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested). |
| Activity | 1.Immobilized Recombinant Human Axl Kinase Protein (ECD, His Tag)(Cat: 10279-H08H-UE) at 0.25 μg/mL (100 μL/well) can bind biotinylated Recombinant Mouse GAS6 Protein (His Tag), the EC 50 is 1-3 ng/mL (Routinely tested). 2.Recombinant Human Axl Kinase Protein (ECD, His Tag) (Cat: 10279-H08H-UE) immobilized on CM5 chip, can bind Recombinant Human GAS6 Protein (His Tag) (Cat: 13170-H07H3) with an affinity constant of 4.388 nM as determined in an SPR assay (Biacore T200) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the extracellular domain of human AXL isoform 1 (AAB20305.1) extracellular domain (Met1-Pro449) was fused with a polyhistidine tag at the C-terminus. |
| Expressed Host | HEK293 Cells |
| Species | Human |
| Predicted N Terminal | Glu 33 |
| Molecule Mass | The secreted recombinant human AXL consists of 428 amino acids after removal of the signal peptide and predicts a molecular mass of 46.5 kDa. As a result of glycosylation, the band migrates approximately 70-75 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 60.7 kDa verified by SEC-MALS(Routinely tested). |
Synonyms: ARK Protein, Human; AXL3 Protein, Human; JTK11 Protein, Human; Tyro7 Protein, Human; UFO Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human AXL Protein 45345 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human AXL Protein 45480 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human AXL Protein 48205 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human AXL Protein 48327 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Human AXL Protein 48346 |
| Image 5 URL | Source image |
Background Information
| Full Name | AXL receptor tyrosine kinase |
| Description | Axl receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. Axl functions in dampening the immune response, regulating cytokine secretion, clearing apoptotic cells and debris, and maintaining cell survival. Axl is upregulated in various disease states, such as in the cuprizone toxicity-induced model of demyelination and in multiple sclerosis (MS) lesions, suggesting that it plays a role in disease pathogenesis. Axl expression correlates with poor prognosis in several cancers. Axl mediates multiple oncogenic phenotypes and activation of these RTKs constitutes a mechanism of chemoresistance in a variety of solid tumors. Axl contributes to cell survival, migration, invasion, metastasis and chemosensitivity justify further investigation of Axl as novel therapeutic targets in cancer. The receptor tyrosine kinase AXL is thought to play a role in metastasis. The soluble AXL receptor as a therapeutic candidate agent for treatment of metastatic ovarian cancer. GAS6/AXL targeting as an effective strategy for inhibition of metastatic tumor progression in vivo. |
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