| Catalog No. | Size | Price (USD) |
| 80025-MM10-A-25 | 25 Tests | $44.43 |
| 80025-MM10-A-100 | 100 Tests | $133.30 |
General Information
| Product name | Anti-B7-2/CD86 Antibody (APC), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Rat |
| Specificity | Rat B7-2/CD86 |
| Immunogen | Recombinant Rat CD86 Protein (Catalog#80025-R08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD86 (rR CD86; Catalog#80025-R08H; NP_064466.1; Met1-Ile250) and conjugated with APC under optimum conditions, the unreacted APC was removed. |
| Source | Monoclonal Mouse IgG2b Clone #10 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Concentration | 10 μl/Test, 0.1 mg/ml |
| Conjugate | APC |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! |
Synonyms: Anti-B7-2 Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of rat CD86 expression on LPS-stimulated SD rat splenocytes. Cells were stained with APC-conjugated anti-rat CD86. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Image 1 Alt Text | Rat CD86 Flow Cytometry (FC) 33910 |
| Image 1 URL | Source image |
Background Information
| Full Name | CD86 antigen |
| Description | CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy. |
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| References |
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title: Discovery of an theranostic functional mAb for visualizing the sensitivity and effectiveness of PD-L1 checkpoint therapy.
authors: Jin Wang; Yanchen Li; ...; Yuanyuan Shan
journal: Bioorganic chemistry
date: 2024-11-22
pmid: 39603072
doi: 10.1016/j.bioorg.2024.107992
cited_by_count: 0
techniques: MTT Assay
bioz_score: 92
title: A bifunctional agent for efficient imaging of PD-L1 and antimelanoma activity.
authors: Jin Wang; Xiaoyan Pan; ...; Yuanyuan Shan
journal: Bioorganic chemistry
date: 2023-11-03
pmid: 37839142
doi: 10.1016/j.bioorg.2023.106912
cited_by_count: 0
techniques: MTT Assay; Nuclear Magnetic Resonance
bioz_score: 92
