| Catalog No. | Size | Price (USD) |
| C29-18G-10 | 10 µg | $515.43 |
| C29-18G-50 | 50 µg | $1,729.93 |
| C29-18G-500 | 500 µg | $3,907.16 |
General Information
| Formulation | Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant full length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | CDK2 ~58 kDa; CyclinE1 ~73kDa |
| Technical Data Sheet by Lot # | F3735-7 F3735-7B K4486-10 K4486-8 K4486-9 O3898-11 Q2306-8 Q2366-10 SCF19JL0231 SCF19JU0331 Z4865-7 Z4873-7 |
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases CDK2/CyclinE1-Cat. No.: C29-18G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human CDK2 & Cyclin E1 Protein 48788 |
| Image 1 URL | Source image |
| Image 2 Description | Determination of ATP Km for recombinant protein kinases CDK2/CyclinE1-Cat. No.: C29-18G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 2 Alt Text | Human CDK2 & Cyclin E1 Protein 49521 |
| Image 2 URL | Source image |
| Image 3 Description | Determination of ATP Km for recombinant protein kinases CDK2/CyclinE1-Cat. No.: C29-18G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 3 Alt Text | Human CDK2 & Cyclin E1 Protein 49522 |
| Image 3 URL | Source image |
title: CDK inhibitors
authors: Zhilong Hu; Hu He; ...; Xiaotian Zhu
date: 2025-04-29
cited_by_count: 0
techniques: Inhibition
bioz_score: 93
title: CDK2 inhibitors and methods of using the same
authors: Louise Clare Kirman; Carl Eric Schwartz; ...; Thomas P. Blaisdell
date: 2024-08-20
cited_by_count: 0
techniques: Activity Assay; Control; Derivative Assay; Inhibition
bioz_score: 93
title: Inhibition of FLT3-ITD Kinase in Acute Myeloid Leukemia by New Imidazo[1,2- b ]pyridazine Derivatives Identified by Scaffold Hopping.
authors: Petra Břehová; Eva Řezníčková; ...; Vladimír Kryštof
journal: Journal of Medicinal Chemistry
date: 2023-08-25
pmid: 37535845
doi: 10.1021/acs.jmedchem.3c00575
cited_by_count: 1
techniques: Concentration Assay; Generated; Produced; Recombinant
bioz_score: 93
title: Discovery of covalent CDK14 inhibitors with pan-TAIRE family specificity
authors: Fleur M. Ferguson; Zainab M. Doctor; ...; Nathanael S. Gray
journal: Cell chemical biology
date: 2020-06-20
pmid: 30930164
doi: 10.1016/j.chembiol.2019.02.015
pmcid: 6588450
cited_by_count: 21
techniques: Binding Assay; Concentration Assay; Homogenization; Inhibition; Kinase Assay; Labeling; Lysis; Recombinant; Sequencing; Software; Western Blot
bioz_score: 93
title: S-Like-Phase Cyclin-Dependent Kinases Stabilize the Epstein-Barr Virus BDLF4 Protein To Temporally Control Late Gene Transcription
authors: Yoshitaka Sato; Takahiro Watanabe; ...; Hiroshi Kimura
journal: Journal of virology
date: 2019-04-03
pmid: 30700607
doi: 10.1128/JVI.01707-18
pmcid: 6450117
cited_by_count: 20
techniques: Activity Assay; Blocking Assay; Expressing; Immunoprecipitation; In Vitro; Incubation; Infection; Liquid Chromatography with Mass Spectroscopy; Luciferase; Magnetic Beads; Mutagenesis; Phosphorylation Assay; Plasmid Preparation; Recombinant; Transfection; Western Blot; shRNA
bioz_score: 93
MDSD
