| Catalog No. | Size | Price (USD) |
| 10001-H08H-1 | 1 mg | $3,576.88 |
| 10001-H08H-10 | 10 µg | $71.09 |
| 10001-H08H-20 | 20 µg | $207.36 |
| 10001-H08H-100 | 100 µg | $573.19 |
| 10001-H08H-500 | 500 µg | $2,229.07 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested). |
| Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc (Cat: 10605-H01H) at 2 μg/ml (100 μl/well) can bind Human EGFR His (Cat: 10001-H08H), the EC 50 of Human EGFR His is 100-500 ng/mL. 2. Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein (Cat. 10001-H08H) with an affinity constant of 1.07 nM as determined in an SPR assay (Biacore T200) (Routinely tested). 3. Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His Tag (Cat. No. 10001-H08H) with an affinity constant of 5.81nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag. |
| Accession# | NP_005219 |
| Expressed Host | HEK293 Cells |
| Species | Human |
| Predicted N Terminal | Leu 25 |
| Molecule Mass | The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 94.2 kDa verified by SEC-MALS(Routinely tested). |
Synonyms: ERBB Protein, Human; ERBB1 Protein, Human; ERRP Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human EGFR/HER1 Protein 8284 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human EGFR Protein 28217 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human EGFR Protein 34657 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human EGFR Protein 35530 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Human EGFR Protein 35838 |
| Image 5 URL | Source image |
Background Information
| Full Name | epidermal growth factor receptor |
| Description | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently. |
| Research Areas |
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| Tags |
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| References |
|
title: Two-Step PEG-Based Surface Functionalization of Gold Nanoparticles Enables Specific SERS Imaging of EGFR in an Ovarian Cancer Cell Model
authors: Alessandro Stumpo; Ricardo Coelho; ...; Sina Saxer
journal: Materials Today Bio
date: 2026-08-01
doi: 10.1016/j.mtbio.2026.103602
cited_by_count: 0
techniques: Incubation
bioz_score: 94
title: Integrated isothermal shift assay and multi-omics identify melittin as a novel EGFR-targeting peptide to suppress NSCLC
authors: Chenhui Zhong; Jietao Gong; ...; Jianyong Huang
journal: Journal of Translational Medicine
date: 2026-06-04
pmid: 42237319
doi: 10.1186/s12967-026-08358-w
cited_by_count: 0
techniques: Binding Assay; Expressing; Fluorescence; Immunofluorescence; Immunohistochemistry; Infection; Knockdown; Phospho-proteomics; Protein-Protein interactions; Recombinant; Shift Assay; Staining; Western Blot
bioz_score: 94
title: An extracellular, optogenetic antibody platform for stimulus-gated antigen recognition and modulation of cell behavior.
authors: Eury Kwon; Daseuli Yu; ...; Won Do Heo
journal: Cell chemical biology
date: 2026-05-07
pmid: 42102802
doi: 10.1016/j.chembiol.2026.04.006
cited_by_count: 0
techniques: High Performance Liquid Chromatography; Luciferase; Recombinant; Transfection
bioz_score: 94
title: Ellagic Acid and Its Nanoparticles Mitigate Atherosclerosis by Elevating Low‐Density Lipoprotein Receptor Levels Through Targeting of the Epidermal Growth Factor Receptor
authors: Guo‐Tao Li; Li‐Tian Wang; ...; Xuan‐Jun Wang
journal: FASEB bioAdvances
date: 2025-11-07
doi: 10.1096/fba.2025-00178
cited_by_count: 0
techniques: Binding Assay; Expressing; Inhibition; Purification; Real-time Polymerase Chain Reaction; Recombinant; SPR Assay; Western Blot
bioz_score: 94
title: Progastrin Promotes Colorectal Cancer Stem Cell‐Like Properties via the Receptor PZR
authors: Julie Nguyen; Marie Lafitte; ...; Serge Roche
journal: Advanced Science
date: 2025-08-03
pmid: 40753649
cited_by_count: 1
techniques: Affinity Column; Affinity Purification; Functional Assay; Purification; Recombinant; Sequencing
bioz_score: 94
title: Antibody binding to human HER2 and preparation method and use thereof
authors: Jianhe Chen; Xuesai Zhang; ...; Zhenping Zhu
date: 2025-06-03
cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay
bioz_score: 94
title: Photoinduced Actin Aggregation Involves Cell Death: A Mechanism of Cancer Cell Cytotoxicity after Near-Infrared Photoimmunotherapy.
authors: Kazuhide Sato; Tomoko Okada; ...; Toshihiko Ogura
journal: ACS Nano
date: 2025-02-18
pmid: 39964399
doi: 10.1021/acsnano.5c00104
cited_by_count: 8
techniques: Recombinant; Software; Staining
bioz_score: 94
title: Multimodal targeting chimeras enable integrated immunotherapy leveraging tumor-immune microenvironment.
authors: Feng Lin; Shenyi Yin; ...; Peng R Chen
journal: Cell
date: 2024-12-27
pmid: 39504957
doi: 10.1016/j.cell.2024.10.016
cited_by_count: 7
techniques: Binding Assay; Concentration Assay; Kinetic Assay; Sequencing; Software
bioz_score: 94
title: Bispecific polypeptides against CD137
authors: Eva Dahlén; Peter Ellmark; ...; Sara Fritzell
date: 2024-12-24
cited_by_count: 0
techniques: Binding Assay; Chromatography; Enzyme-linked Immunosorbent Assay; Produced; Purification; Transfection
bioz_score: 94
title: Synthetic GPCRs for programmable sensing and control of cell behaviour
authors: Nicholas A. Kalogriopoulos; Reika Tei; ...; Alice Ting
journal: Nature
date: 2024-12-04
pmid: 39633047
doi: 10.1038/s41586-024-08282-3
pmcid: 11666456
cited_by_count: 17
bioz_score: 94
title: A molecular toolkit for heterologous protein secretion across Bacteroides species
authors: Yu-Hsuan Yeh; Vince W. Kelly; ...; Shannon J. Sirk
journal: Nature Communications
date: 2024-11-11
doi: 10.1038/s41467-024-53845-7
pmcid: 11554821
cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay; Incubation; Purification; Western Blot
bioz_score: 94
title: SIBP-03, a novel anti-HER3 antibody, exerts antitumor effects and synergizes with EGFR- and HER2-targeted drugs.
authors: Wen-Jing Li; Cheng-Ying Xie; ...; Li-Guang Lou
journal: Acta pharmacologica Sinica
date: 2024-03-18
pmid: 38200149
doi: 10.1038/s41401-023-01221-4
cited_by_count: 5
techniques: Recombinant
bioz_score: 94
title: Ellagic acid ameliorates atherosclerosis by targeting the epidermal growth factor receptor
authors: Huang Ye-Wei; Wang Li-Tian; ...; Wang Xuan-Jun
journal: bioRxiv
date: 2023-09-15
doi: 10.1101/2023.09.14.557846
cited_by_count: 0
techniques: Binding Assay; Blocking Assay; Immunohistochemistry; MTT Assay; Real-time Polymerase Chain Reaction; Recombinant; Reverse Transcription Polymerase Chain Reaction; SPR Assay; SYBR Green Assay; Staining
bioz_score: 94
title: Enzymes Photocrosslinked to Live Cell Receptors Retain Activity and EGFR Inhibition After Both Internalization and Recycling
authors: Shambojit Roy; Michael Brasino; ...; Andrew P. Goodwin
journal: Bioconjugate Chemistry
date: 2023-06-22
pmid: 31840981
doi: 10.1021/acs.bioconjchem.9b00781
pmcid: 10286729
cited_by_count: 5
techniques: Labeling; Polymerase Chain Reaction; Purification; SDS Page; Staining
bioz_score: 94
title: Design and selection of optimal ErbB-targeting bispecific antibodies in pancreatic cancer.
authors: Emilia Rabia; Véronique Garambois; ...; Thierry Chardès
journal: Frontiers in Immunology
date: 2023-05-10
pmid: 37153618
doi: 10.3389/fimmu.2023.1168444
cited_by_count: 7
techniques: Activation Assay; Binding Assay; Cell Culture; Comparison; Control; Cytometry; Enzyme-linked Immunosorbent Assay; Expressing; Incubation; Labeling; Lysis; Molecular Weight; Negative Control; Phospho-proteomics; Positive Control; Proliferation Assay; Recombinant; SDS Page; Sandwich ELISA; Staining; Transfection
bioz_score: 94
title: Sequence enrichment profiles enable target-agnostic antibody generation for a broad range of antigens
authors: Jenny Mattsson; Anne Ljungars; ...; Björn Frendéus
journal: Cell Reports Methods
date: 2023-05-09
doi: 10.1016/j.crmeth.2023.100475
pmcid: 10261905
cited_by_count: 2
techniques: Produced; Recombinant; Sequencing; Software
bioz_score: 94
title: Expanding the Therapeutic Window of EGFR-Targeted PE24 Immunotoxin for EGFR-Overexpressing Cancers by Tailoring the EGFR Binding Affinity
authors: Sei-Yong Jun; Dae-Seong Kim; ...; Alessandro Poggi
journal: International Journal of Molecular Sciences
date: 2022-12-13
pmcid: 9779439
cited_by_count: 4
techniques: Binding Assay; Blocking Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Expressing; Incubation; Purification; Recombinant; SDS Page; Staining
bioz_score: 94
title: An FGFR1-Binding Peptide Modified Liposome for siRNA Delivery in Lung Cancer
authors: Zhipeng Dong; Yunxue Yin; ...; Aamir Ahmad
journal: International Journal of Molecular Sciences
date: 2022-07-29
pmid: 35955516
doi: 10.3390/ijms23158380
pmcid: 9369135
cited_by_count: 5
techniques: Fluorescence; Lysis; MTT Assay; Modification; Negative Control; Synthesized
bioz_score: 94
title: Novel cancer‐specific epidermal growth factor receptor antibody obtained from the serum of esophageal cancer patients with long‐term survival
authors: Sayaka Takagi‐Maeda; Satoshi Yajima; ...; Hideaki Shimada
journal: Cancer Science
date: 2022-04-18
pmid: 35348270
doi: 10.1111/cas.15350
pmcid: 9207364
cited_by_count: 4
techniques: Blocking Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Incubation; Recombinant
bioz_score: 94
title: In Silico and In Vitro Screening of 50 Curcumin Compounds as EGFR and NF-κB Inhibitors
authors: Mohamed E. M. Saeed; Rümeysa Yücer; ...; Biji Theyilamannil Kurien
journal: International Journal of Molecular Sciences
date: 2022-04-02
pmid: 35409325
doi: 10.3390/ijms23073966
pmcid: 9000198
cited_by_count: 43
techniques: Microscale Thermophoresis; Recombinant
bioz_score: 94
title: HER2-targeted dual radiotracer approach with clinical potential for noninvasive imaging of trastuzumab-resistance caused by epitope masking
authors: Liqiang Li; Tianyu Liu; ...; Fan Wang
journal: Theranostics
date: 2022-01-01
pmid: 35910795
doi: 10.7150/thno.74154
pmcid: 9330517
cited_by_count: 14
techniques: Recombinant; Variant Assay
bioz_score: 94
title: Combination of cassette-dosing and microsampling for reduced animal usage for antibody pharmacokinetics in cynomolgus monkeys, wild-type mice, and human FcRn transgenic mice.
authors: Miho Nagayasu; Kazuhisa Ozeki
journal: Pharmaceutical Research
date: 2021-11-08
pmid: 33782838
doi: 10.1007/s11095-021-03028-6
cited_by_count: 7
techniques: Blocking Assay; Concentration Assay; Injection; Recombinant
bioz_score: 94
title: Cetuximab–siRNA Conjugate Linked Through Cationized Gelatin Knocks Down KRAS G12C Mutation in NSCLC Sensitizing the Cells Toward Gefitinib
authors: K. Sreedurgalakshmi; R. Srikar; ...; Reena Rajkumari
journal: Technology in cancer research & treatment
date: 2021-09-20
pmid: 34542333
doi: 10.1177/15330338211041453
pmcid: 8461128
cited_by_count: 15
bioz_score: 94
title: Chimeric STAR receptors using TCR machinery mediate robust responses against solid tumors.
authors: Yue Liu; Guangna Liu; ...; Xin Lin
journal: Science translational medicine
date: 2021-07-12
pmid: 33762437
doi: 10.1126/scitranslmed.abb5191
cited_by_count: 85
techniques: Flow Cytometry; Incubation; Staining
bioz_score: 94
title: Crystal structures of HER3 extracellular domain 4 in complex with the designed ankyrin-repeat protein D5
authors: Filip Radom; Clemens Vonrhein; ...; Andreas Plückthun
journal: Acta Crystallographica. Section F, Structural Biology Communications
date: 2021-07-01
pmid: 34196609
doi: 10.1107/S2053230X21006002
pmcid: 8248824
cited_by_count: 1
techniques: Binding Assay; Concentration Assay; Incubation
bioz_score: 94
title: T cell recruiting polypeptides based on TCR alpha/beta reactivity
authors: Annelies Roobrouck; Diane Van Hoorick; João Vieira
date: 2021-02-23
cited_by_count: 0
techniques: Binding Assay; Injection; Purification; SPR Assay
bioz_score: 94
title: Enzymes Photo-Cross-Linked to Live Cell Receptors Retain Activity and EGFR Inhibition after Both Internalization and Recycling.
authors: Shambojit Roy; Michael Brasino; ...; Andrew P Goodwin
journal: Bioconjugate Chemistry
date: 2021-02-04
pmid: 31840981
cited_by_count: 6
techniques: Labeling; Polymerase Chain Reaction; Purification; Staining
bioz_score: 94
title: Nucleic acid-polypeptide compositions and uses thereof
authors: Andrew John Geall; Venkata Ramana Doppalapudi; ...; Beatrice Diana Darimont
date: 2020-10-13
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Fluorescence; Formulation; In Vitro; Incubation; Purification
bioz_score: 94
title: VHH characterization.Recombinant VHHs: Production, characterization and affinity.
authors: Eric Chabrol; Johann Stojko; ...; Jean A Boutin
journal: Analytical biochemistry
date: 2020-09-22
pmid: 31676284
doi: 10.1016/j.ab.2019.113491
cited_by_count: 17
techniques: Injection; Recombinant
bioz_score: 94
title: Effects of viral promoters, the Woodchuck hepatitis post-transcriptional regulatory element, and weakened antibiotic resistance markers on transgene expression in Chinese hamster ovary cells
authors: Wen Wang; Xiao Guo; ...; Yan-Fang Wang
journal: Process Biochemistry
date: 2020-07-01
doi: 10.1016/j.procbio.2020.04.005
cited_by_count: 1
techniques: Clone Assay; Expressing; Incubation; Western Blot
bioz_score: 94
title: Retargeting CD19 Chimeric Antigen Receptor T Cells via Engineered CD19-Fusion Proteins.
authors: Justin R Klesmith; Lihe Su; ...; Paul D Rennert
journal: Molecular Pharmaceutics
date: 2020-06-25
pmid: 31242389
doi: 10.1021/acs.molpharmaceut.9b00418
cited_by_count: 25
techniques: Cell Analysis; FACS; Generated; Luciferase; Modification; Produced; Real-time Polymerase Chain Reaction; Selection; Sequencing
bioz_score: 94
title: Bridging the Clinical Gap for DNA-Based Antibody Therapy Through Translational Studies in Sheep.
authors: Kevin Hollevoet; Stéphanie De Vleeschauwer; ...; Paul Declerck
journal: Human gene therapy
date: 2020-03-27
pmid: 31382777
doi: 10.1089/hum.2019.128
cited_by_count: 17
techniques: Enzyme-linked Immunosorbent Assay; Purification
bioz_score: 94
title: Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system
authors: Satoshi Murakami; Rena Matsumoto; Takashi Kanamori
journal: Scientific Reports
date: 2019-01-24
pmid: 30679500
doi: 10.1038/s41598-018-36691-8
pmcid: 6345822
cited_by_count: 25
techniques: Enzyme-linked Immunosorbent Assay; Recombinant; Synthesized
bioz_score: 94
title: Peripheral White Blood Cell Subsets in Metastatic Colorectal Cancer Patients Treated with Cetuximab: The Potential Clinical Relevance
authors: Ivana Z. Matić; Branka Kolundžija; ...; Zorica D. Juranić
journal: Frontiers in Immunology
date: 2018-01-04
pmid: 29354119
doi: 10.3389/fimmu.2017.01886
pmcid: 5758541
cited_by_count: 8
techniques: Enzyme-linked Immunosorbent Assay; Recombinant
bioz_score: 94
title: Methods for assessing and identifying or evolving conditionally active therapeutic proteins
authors: Lalitha Kodandapani; Louis H. Bookbinder; ...; Lei Huang
date: 2017-06-20
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Direct ELISA; Incubation
bioz_score: 94
title: Erbin interacts with c-Cbl and promotes tumourigenesis and tumour growth in colorectal cancer by preventing c-Cbl-mediated ubiquitination and down-regulation of EGFR.
authors: Su Yao; Ping Zheng; ...; Jian-Ming Li
journal: The Journal of pathology
date: 2015-07-15
pmid: 25521828
doi: 10.1002/path.4502
cited_by_count: 37
bioz_score: 94
title: Endothelial Cells Control Pancreatic Cell Fate at Defined Stages through EGFL7 Signaling
authors: Der-I Kao; Lauretta A. Lacko; ...; Shuibing Chen
journal: Stem Cell Reports
date: 2015-01-15
pmid: 25601205
doi: 10.1016/j.stemcr.2014.12.008
pmcid: 4325230
cited_by_count: 26
techniques: Binding Assay; Fluorescence; Labeling; Recombinant; Titration
bioz_score: 94
title: Phage-derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGF.
authors: Juan Zhang; Haixin Li; ...; Min Wang
journal: Biotechnology progress
date: 2012-12-10
pmid: 22581629
doi: 10.1002/btpr.1559
cited_by_count: 17
techniques: Enzyme-linked Immunosorbent Assay; Modification; Produced
bioz_score: 94
