Anti-CDK1/CDC2 Antibody, Rabbit Polyclonal sinobiological 109678-T36
$243.00
In stock
SKU
109678-T36
| Catalog No. | Size | Price (USD) |
| 109678-T36-100 | 100 µL | $251.64 |
General Information
| Product name | Anti-CDK1/CDC2 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CDK1/CDC2 |
| Immunogen | A synthetic peptide corresponding to the center region of the Human CDK1 |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CDK1, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CDC2 Antibody; Anti-CDC28A Antibody; Anti-CDKN1 Antibody; Anti-P34CDC2 Antibody
Image / Experimental Information
| Image 1 Description | Anti-CDK1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:34 kDa |
| Image 1 Alt Text | Human CDK1 Western blot (WB) 38617 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-CDK1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:34 kDa |
| Image 2 Alt Text | Human CDK1 Immunoprecipitation(IP) 38618 |
| Image 2 URL | Source image |
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Background Information
| Full Name | cyclin-dependent kinase 1 |
| Description | CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors. |
| References |
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