| Catalog No. | Size | Price (USD) |
| 13052-HCCS-50 | 50 µg | $342.14 |
| 13052-HCCS-100 | 100 µg | $573.19 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | > 85 % as determined by SDS-PAGE |
| Activity | Testing in progress |
| Protein Construction | A DNA sequence encoding the human KIR2DL4 (ADY38409.1)(Met1-His242) was expressed with six amino acids (LEVLFQ) at the C-terminus was expressed and purified. |
| Accession# | ADY38409.1 |
| Expressed Host | CHO Stable Cells |
| Species | Human |
| Predicted N Terminal | Trp 24 |
| Molecule Mass | The recombinant human KIR2DL4 consists of 228 amino acids and predicts a molecular mass of 25.1 KDa. It migrates as an approximately 33-37 KDa band in SDS-PAGE under reducing conditions. |
Synonyms: 103AS Protein, Human; 15.212 Protein, Human; CD158D Protein, Human; G9P Protein, Human; KIR-103AS Protein, Human; KIR-2DL4 Protein, Human; KIR103 Protein, Human; KIR103AS Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human KIR2DL4 / CD158D Protein 10153 |
| Image 1 URL | Source image |
Background Information
| Full Name | killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4 |
| Description | KIR2DL4, also known as CD158d, is a member of the killer cell Ig-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous. KIR2DL4 is expressed in all NK cells and some T cells. KIR2DL4 activates the cytotoxicity of NK cells, despite the presence of an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail. The ITIM was not necessary for activation of lysis by KIR2DL4. The activation signal of KIR2DL4 was sensitive to inhibition by another ITIM-containing receptor. The activation-deficient mutant of KIR2DL4 inhibited the signal delivered by the activating receptor CD16. |
| Research Areas |
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| References |
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title: BiTE-Secreting CAR-γδT as a Dual Targeting Strategy for the Treatment of Solid Tumors.
authors: Shi-Wei Huang; Chih-Ming Pan; ...; Der-Yang Cho
journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
date: 2023-06-15
pmid: 37078788
doi: 10.1002/advs.202206856
cited_by_count: 32
techniques: Activity Assay; Binding Assay; Bioassay; Blocking Assay; Clone Assay; Competitive ELISA; Construct; Derivative Assay; Enzyme-linked Immunosorbent Assay; Gradient Centrifugation; Incubation; Isolation; Recombinant; Saline
bioz_score: 93
title: BiTE‐Secreting CAR‐ γδ T as a Dual Targeting Strategy for the Treatment of Solid Tumors
authors: Shi‐Wei Huang; Chih‐Ming Pan; ...; Der‐Yang Cho
journal: Advanced Science
date: 2023-04-20
pmid: 37078788
pmcid: 10265101
cited_by_count: 32
techniques: Activity Assay; Binding Assay; Blocking Assay; Clone Assay; Competitive ELISA; Construct; Cytotoxicity Assay; Derivative Assay; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Functional Assay; Incubation; Modification; Recombinant
bioz_score: 93
