| Catalog No. | Size | Price (USD) |
| 16498-HNAH-B-20 | 20 µg | $293.26 |
| 16498-HNAH-B-100 | 100 µg | $693.16 |
General Information
| Formulation | Lyophilized from sterile PBS, pH7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
| Activity | 1.Immobilized Recombinant Human LAG3/CD223 Protein,Biotinylated(ECD) (Cat: 16498-HNAH-B）at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-LAG3 Antibody, the EC 50 is 5-15 ng/mL.（QC tested） 2.Immobilized Recombinant Human LAG3/CD223 Protein,Biotinylated(ECD) (Cat: 16498-HNAH-B）at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human FGL1 Protein (His Tag) (Cat: 13484-H08B), the EC 50 is 20-65 ng/mL.（routinely tested） |
| Protein Construction | A DNA sequence encoding the Human LAG3 (P18627) (Met1-Arg440) was expressed. The expressed protein was biotinylated in vitro. |
| Accession# | NP_002277.4 |
| Expressed Host | HEK293 Cells |
| Species | Human |
| Predicted N Terminal | Leu 23 |
| Molecule Mass | The recombinant human LAG3 consists of 418 amino acids and predicts a molecular mass of 45.3 kDa. As a result of glycosylation, it migrates as an approximately 47.0 KDa band in SDS-PAGE under non-reducing conditions. |
Synonyms: CD223 Protein, Human; FDC Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human LAG3/LAG-3/CD223 Protein 18612 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human LAG-3 Protein 39395 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human LAG-3 Protein 39396 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human LAG-3 Protein 41667 |
| Image 4 URL | Source image |
Background Information
| Full Name | lymphocyte-activation gene 3 |
| Description | LAG3 (Lymphocyte Activating 3) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease. |
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