| Catalog No. | Size | Price (USD) |
| 40068-RP01-20 | 20 µL | $88.87 |
| 40068-RP01-100 | 100 µL | $251.64 |
| 40068-RP01-200 | 200 µL | $370.28 |
General Information
| Product name | MERS-CoV Nucleoprotein / NP Antibody, Rabbit PAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP |
| Immunogen | Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (Catalog#40068-V08B) |
| Preparation | Produced in rabbits immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (Catalog#40068-V08B; AFS88943.1; Met1-Asp413). Total IgG was purified by Protein A affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| References |
|
title: Linking biochemical and cellular efficacy of MERS coronavirus main protease inhibitors
authors: Van N. T. La; Noa Lahav; ...; David D. L. Minh
journal: ACS pharmacology & translational science
date: 2026-06-24
pmid: 42422564
doi: 10.1021/acsptsci.6c00107
cited_by_count: 0
bioz_score: 95
title: MERS-CoV and SARS-CoV-2 infection in diverse human lung organoid-derived cultures
authors: Kim Lam Chiok; Kristof Jenik; ...; Tom Gallagher
journal: Journal of virology
date: 2025-10-01
pmid: 40965144
cited_by_count: 2
bioz_score: 95
title: Bat-specific adaptations in interferon signaling and GBP1 contribute to enhanced antiviral capacity
authors: Victoria Gonzalez; Briallen Lobb; ...; Arinjay Banerjee
journal: Nature Communications
date: 2025-07-01
pmid: 40593754
cited_by_count: 3
techniques: Biomarker Discovery; Chromatin Immunoprecipitation; Flow Cytometry; Generated; Magnetic Resonance Imaging; Western Blot
bioz_score: 95
title: Expanding the bat toolbox: Carollia perspicillata bat cell lines and reagents enable the characterization of viral susceptibility and innate immune responses
authors: Victoria Gonzalez; Cierra Word; ...; Katherine Kedzierska
journal: PLoS Biology
date: 2025-04-15
pmcid: 11999112
cited_by_count: 0
techniques: Blocking Assay; Confocal Microscopy; Infection; Labeling; Membrane; Microscopy; Molecular Weight; SDS Page; Software; Staining; TCID50 Assay; Transfection; Virus; Western Blot
bioz_score: 95
title: Viral susceptibility and innate immune competency of Carollia perspicillata bat cells produced for virological studies
authors: Gonzalez Victoria; Word Cierra; ...; Letko Michael
journal: bioRxiv
date: 2024-11-19
doi: 10.1101/2024.11.19.624190
cited_by_count: 0
techniques: Blocking Assay; Membrane; SDS Page; Software; Western Blot
bioz_score: 95
title: Biologically Active Micropatterns of Biomolecules and Living Matter Using Microbubble Lithography.
authors: Anand Dev Ranjan; Sucharita Bhowmick; ...; Ayan Banerjee
journal: Small (Weinheim an Der Bergstrasse, Germany)
date: 2024-10-18
pmid: 38884187
doi: 10.1002/smll.202401127
cited_by_count: 6
techniques: Fluorescence; Incubation; Virus
bioz_score: 95
title: Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease
authors: Kejia Zhang; Patrick Eldin; ...; Dominique Soldati-Favre
journal: eLife
date: 2024-05-30
pmid: 38814682
doi: 10.7554/eLife.90316
pmcid: 11139479
cited_by_count: 11
techniques: Endpoint Dilution Assay; Expressing; Generated; Imaging; Infection; Isolation; Knock-Out; Liquid Chromatography with Mass Spectroscopy; Modification; Plasmid Preparation; Protein Purification; Quantitative RT-PCR; RNA modification; Recombinant; Sequencing; Software; Strep-tag; Suspension; Variant Assay; Virus; Western Blot
bioz_score: 95
title: Quantitative assay to analyze neutralization and inhibition of authentic Middle East respiratory syndrome coronavirus
authors: Helena Müller-Kräuter; Jolanda Mezzacapo; ...; Verena Krähling
journal: Medical Microbiology and Immunology
date: 2024-05-09
pmid: 38722338
doi: 10.1007/s00430-024-00789-w
pmcid: 11082005
cited_by_count: 1
techniques: Comparison; Concentration Assay; Generated; Inhibition; Microneutralization Assay; Staining; Two Tailed Test; Virus
bioz_score: 95
title: Cross-protective HCoV immunity reduces symptom development during SARS-CoV-2 infection
authors: Irene A. Abela; Magdalena Schwarzmüller; ...; Satya Dandekar
journal: mBio
date: 2024-02-01
pmid: 38270455
doi: 10.1128/mbio.02722-23
pmcid: 10865973
cited_by_count: 8
techniques: Clinical Proteomics; Concentration Assay; Control; Expressing; Sampling
bioz_score: 95
title: Broad-spectrum antiviral activity of two structurally analogous CYP3A inhibitors against pathogenic human coronaviruses in vitro.
authors: Lara Gallucci; James Bazire; ...; Iart Luca Shytaj
journal: Antiviral research
date: 2023-11-30
pmid: 38042417
doi: 10.1016/j.antiviral.2023.105766
cited_by_count: 4
techniques: Imaging; Immunofluorescence; Incubation; Infection; Inhibition; Software; Staining
bioz_score: 95
title: Broad-spectrum antiviral activity of clinically approved CYP3A inhibitors against pathogenic human coronaviruses in vitro
authors: Gallucci Lara; Bazire James; ...; Shytaj Iart Luca
journal: bioRxiv
date: 2023-08-23
doi: 10.1101/2023.08.23.554463
cited_by_count: 0
techniques: Imaging; Immunofluorescence; Incubation; Infection; Software; Staining
bioz_score: 95
title: Methods for producing and using IgY antibodies targeting the middle east respiratory syndrome coronavirus spike protein to treat or prevent MERS-CoV infection
authors: Esam Ibraheem Azhar; Sherif Ali El-Kafrawy; ...; Ashraf Abdu Tabil
date: 2022-05-03
cited_by_count: 0
techniques: Activity Assay; Immunohistochemistry; Infection; Polymer; Software; Staining
bioz_score: 95
title: An Integrated Platform for Serological Detection and Vaccination of COVID-19
authors: Sung-Chan Wei; Wei-Ting Hsu; ...; Yu-Chan Chao
journal: Frontiers in Immunology
date: 2021-12-23
pmid: 35003088
doi: 10.3389/fimmu.2021.771011
pmcid: 8734241
cited_by_count: 7
techniques: Concentration Assay; Enzyme-linked Immunosorbent Assay; In-Cell ELISA; Infection; Positive Control
bioz_score: 95
title: MERS-CoV infection causes brain damage in human DPP4-transgenic mice through complement-mediated inflammation.
authors: Yuting Jiang; Yuehong Chen; ...; Shihui Sun
journal: The Journal of general virology
date: 2021-11-04
pmid: 34704923
doi: 10.1099/jgv.0.001667
cited_by_count: 10
techniques: Avidin-Biotin Assay; Immunofluorescence; Immunohistochemistry; Incubation; Marker; Microscopy; Saline; Staining
bioz_score: 95
title: Immunoprecipitation-targeted proteomics assays facilitate rational development of SARS-CoV-2 serological diagnostics
authors: Fu Zhiqiang; Rais Yasmine; Drabovich Andrei P.
journal: medRxiv
date: 2021-10-26
doi: 10.1101/2021.10.25.21265408
cited_by_count: 0
techniques: Binding Assay; Recombinant
bioz_score: 95
title: Predictive Value of Precision-Cut Lung Slices for the Susceptibility of Three Animal Species for SARS-CoV-2 and Validation in a Refined Hamster Model
authors: Nora M. Gerhards; Jan B. W. J. Cornelissen; ...; Klaus Schughart
journal: Pathogens
date: 2021-06-30
pmid: 34209230
doi: 10.3390/pathogens10070824
pmcid: 8308566
cited_by_count: 31
techniques: Activity Assay; Expressing; Histopathology; Immunofluorescence; Immunohistochemistry; Infection; Inhibition; Injection; Isolation; Negative Control; Neutralization; Quantitative RT-PCR; Staining; Standard Deviation; Virus Isolation Assay
bioz_score: 95
title: Immunotherapeutic Efficacy of IgY Antibodies Targeting the Full-Length Spike Protein in an Animal Model of Middle East Respiratory Syndrome Coronavirus Infection
authors: Sherif A. El-Kafrawy; Aymn T. Abbas; ...; Juan Carlos Saiz
journal: Pharmaceuticals
date: 2021-05-26
pmid: 34073502
doi: 10.3390/ph14060511
pmcid: 8229159
cited_by_count: 14
techniques: Activity Assay; Binding Assay; Histopathology; Immunohistochemistry; Infection; MANN-WHITNEY; Molecular Weight; Neutralization; Purification; Recombinant; SDS Page; Staining; Transgenic Assay; Western Blot
bioz_score: 95
title: Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
authors: Helen E. Everett; Fabian Z. X. Lean; ...; Karla Helbig
journal: Viruses
date: 2021-01-15
pmid: 33467732
doi: 10.3390/v13010113
pmcid: 7830262
cited_by_count: 66
techniques: Histopathology; Hybridization; Immunohistochemistry; In Situ; Incubation
bioz_score: 95
title: Anti-S1 MERS-COV IgY Specific Antibodies Decreases Lung Inflammation and Viral Antigen Positive Cells in the Human Transgenic Mouse Model
authors: Aymn T. Abbas; Sherif A. El-Kafrawy; ...; Esam I. Azhar
journal: Vaccines
date: 2020-11-01
pmid: 33139631
doi: 10.3390/vaccines8040634
pmcid: 7712919
cited_by_count: 13
techniques: Activity Assay; Adsorption; Binding Assay; Concentration Assay; Formalin-fixed Paraffin-Embedded; Histopathology; Immunofluorescence; Immunohistochemistry; Incubation; Infection; Inhibition; MANN-WHITNEY; Marker; Neutralization; Plaque Reduction Neutralization Test; Purification; Recombinant; SDS Page; Staining; Transgenic Assay; Western Blot
bioz_score: 95
title: Selection of viral variants during persistent infection of insectivorous bat cells with Middle East respiratory syndrome coronavirus
authors: Arinjay Banerjee; Sonu Subudhi; ...; Vikram Misra
journal: Scientific Reports
date: 2020-04-29
pmid: 32350357
doi: 10.1038/s41598-020-64264-1
pmcid: 7190632
cited_by_count: 19
techniques: Western Blot
bioz_score: 95
title: The characteristics of hDPP4 transgenic mice subjected to aerosol MERS coronavirus infection via an animal nose‐only exposure device
authors: Xin‐yan Hao; Qi Lv; ...; Hong Gao
journal: Animal models and experimental medicine
date: 2019-10-30
pmid: 31942559
doi: 10.1002/ame2.12088
pmcid: 6930991
cited_by_count: 15
techniques: Expressing; Histopathology; Immunohistochemical staining; Staining
bioz_score: 95
title: Species-Specific Colocalization of Middle East Respiratory Syndrome Coronavirus Attachment and Entry Receptors
authors: W. Widagdo; Nisreen M. A. Okba; ...; Mark T. Heise
journal: Journal of virology
date: 2019-06-05
pmid: 31167913
doi: 10.1128/JVI.00107-19
pmcid: 6675889
cited_by_count: 27
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Expressing; Immunofluorescence; Incubation; Infection; Staining
bioz_score: 95
title: Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus
authors: Naoko Iwata-Yoshikawa; Tadashi Okamura; ...; Tom Gallagher
journal: Journal of virology
date: 2019-01-09
pmid: 30626685
doi: 10.1128/JVI.01818-18
pmcid: 6401458
cited_by_count: 32
techniques: Cell Culture; Double Staining; Expressing; Formalin-fixed Paraffin-Embedded; Histopathology; Immunofluorescence; Immunohistochemical staining; Immunohistochemistry; Infection; Isolation; Quantitative RT-PCR; Real-time Polymerase Chain Reaction; Staining; Standard Deviation; Transgenic Assay; Virus Isolation Assay
bioz_score: 95
title: Inhibition of Cytosolic Phospholipase A 2 α Impairs an Early Step of Coronavirus Replication in Cell Culture
authors: Christin Müller; Martin Hardt; ...; John Ziebuhr
journal: Journal of virology
date: 2018-02-15
doi: 10.1128/jvi.01463-17
cited_by_count: 96
techniques: Incubation
bioz_score: 95
title: Multi-Organ Damage in Human Dipeptidyl Peptidase 4 Transgenic Mice Infected with Middle East Respiratory Syndrome-Coronavirus
authors: Guangyu Zhao; Yuting Jiang; ...; Stefan Pöhlmann
journal: PLoS ONE
date: 2015-12-23
pmid: 26701103
doi: 10.1371/journal.pone.0145561
pmcid: 4689477
cited_by_count: 68
techniques: Binding Assay; Clone Assay; Expressing; Histopathology; Immunofluorescence; Immunohistochemical staining; Immunohistochemistry; In Vitro; Infection; Labeling; Marker; Plasmid Preparation; Quantitative RT-PCR; Staining; Transfection; Transgenic Assay
bioz_score: 95
