| Catalog No. | Size | Price (USD) |
| 40143-MM05-B-20 | 20 µg | $177.73 |
| 40143-MM05-B-100 | 100 µg | $555.42 |
General Information
| Product name | SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody (Biotin), Mouse MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV Nucleocapsid |
| Immunogen | Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422) and then biotinylated. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and then biotinylated. |
| Source | Monoclonal Mouse IgG1 Clone #05 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Biotin |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| References |
|
title: Engineered Affinity Proteins Enable Sensitive Label-Free Electrochemical Detection
authors: Walaa Khushaim; Khaled Abd El-Rahman; ...; Hadley D. Sikes
journal: Biosensors and Bioelectronics: X
date: 2026-08-01
doi: 10.1016/j.biosx.2026.100832
cited_by_count: 0
bioz_score: 94
title: Update and reuse: Structure-guided nanobody evolution against SARS-CoV-2 escape.
authors: Fan Bu; Divyasha Saxena; ...; Fang Li
journal: PLoS Pathogens
date: 2026-05-21
pmid: 42149970
doi: 10.1371/journal.ppat.1014223
cited_by_count: 0
techniques: In Vivo; Microneutralization Assay; Staining
bioz_score: 94
title: Cell type-specific interactions induce tonic interferon production in endothelial cells in a pathogen-independent manner
authors: Timothy Surette; Fiamma Serra; ...; Dagmar Wirth
journal: Cell Communication and Signaling : CCS
date: 2026-01-13
pmid: 41527090
doi: 10.1186/s12964-026-02650-4
cited_by_count: 0
techniques: Blocking Assay; Co-Culture Assay; Control; Immunostaining; Infection; Microscopy; Software; Staining
bioz_score: 94
title: Unlocking the potential of phytochemicals in inhibiting SARS-CoV-2 M Pro protein - an in silico and cell-based approach
authors: Khushboo Singh; J. J. Patten; ...; Jesse Leverett
journal: Scientific Reports
date: 2025-07-02
pmid: 40596019
doi: 10.1038/s41598-025-05907-z
cited_by_count: 0
techniques: Construct; Incubation; Infection; Staining; Virus
bioz_score: 94
title: SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell-derived human neurons.
authors: Jasmina M Luczo; Sarah J Edwards; ...; Vinod Sundaramoorthy
journal: Journal of neurovirology
date: 2024-01-03
pmid: 38172412
cited_by_count: 4
techniques: Imaging; Immunocytochemistry; Immunofluorescence; Incubation; Marker; Microscopy; Modification; Staining
bioz_score: 92
title: Murine COVID-19 lethality is characterized by lymphoid depletion associated with suppressed antigen presenting cell functionality
authors: Yu Jin Lee; Sang Hyeok Seok; ...; Je Kyung Seong
journal: The American Journal of Pathology
date: 2023-04-05
doi: 10.1016/j.ajpath.2023.03.008
pmcid: 10073095
cited_by_count: 2
techniques: Derivative Assay; Expressing; Flow Cytometry; Imaging; Immunofluorescence; Immunohistochemistry; Incubation; Infection; Marker; Microscopy; Reverse Transcription Polymerase Chain Reaction; Staining; TUNEL Assay; Transgenic Assay; Variant Assay
bioz_score: 94
title: Functional analyses and single cell immunoprofiling uncover sex-specific differences in SARS-CoV2 immune memory development
authors: Basak Eraslan; Eric Brown; ...; Ramnik Xavier
journal: Europe PMC
date: 2022-03-15
doi: 10.21203/rs.3.rs-1416969
cited_by_count: 0
techniques: Control; Expressing; Fluorescence; High Content Screening; Imaging; Incubation; Isolation; Luciferase; Neutralization; Staining; Virus
bioz_score: 92
title: Vascular Inflammation Is Associated with Loss of Aquaporin 1 Expression on Endothelial Cells and Increased Fluid Leakage in SARS-CoV-2 Infected Golden Syrian Hamsters
authors: Lisa Allnoch; Georg Beythien; ...; Luis Martinez-Sobrido
journal: Viruses
date: 2021-04-08
pmid: 33918079
doi: 10.3390/v13040639
pmcid: 8069375
cited_by_count: 44
techniques: Electron Microscopy; Expressing; Immunohistochemistry; Immunolabeling; Immunostaining; Infection; Labeling; Light Microscopy; MANN-WHITNEY; Staining; Transmission Assay; Whisker Assay
bioz_score: 94
title: The SARS-CoV-2 envelope and membrane proteins modulate maturation and retention of the spike protein, allowing assembly of virus-like particles
authors: Bertrand Boson; Vincent Legros; ...; Solène Denolly
journal: Journal of Biological Chemistry
date: 2021-01-01
doi: 10.1074/jbc.ra120.016175
cited_by_count: 202
bioz_score: 93
title: The SARS-CoV-2 Envelope and Membrane proteins modulate maturation and retention of the Spike protein, allowing optimal formation of VLPs in presence of Nucleoprotein
authors: Boson Bertrand; Legros Vincent; ...; Denolly Solène
journal: bioRxiv
date: 2020-08-24
doi: 10.1101/2020.08.24.260901
cited_by_count: 0
techniques: Cell-Cell Fusion Assay; Confocal Microscopy; Expressing; Infection; Plasmid Preparation; Transfection; Western Blot
bioz_score: 92
