| Catalog No. | Size | Price (USD) |
| 40143-R001-B-20 | 20 µg | $177.73 |
| 40143-R001-B-100 | 100 µg | $555.42 |
General Information
| Product name | SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody (Biotin), Rabbit MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV Nucleocapsid |
| Immunogen | Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B) |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells and then biotinylated. |
| Source | Monoclonal Rabbit IgG Clone #001 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Biotin |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| References |
|
title: Disruption of spike protein N-glycosylation induces its endoplasmic reticulum retention and attenuates SARS-CoV-2 infectivity
authors: Weili Kong; Jiali Zhang; ...; Ryan A. Langlois
journal: Journal of virology
date: 2026-04-01
pmid: 41910405
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Cell Culture; Centrifugation; Clinical Proteomics; Co-Immunoprecipitation Assay; Concentration Assay; Control; Derivative Assay; Expressing; Flow Cytometry; Fluorescence; Focus Forming Assay; Functional Assay; Glycoproteomics; Immunofluorescence; Immunoprecipitation; Incubation; Infection; Inhibition; Knock-Out; Knockdown; Labeling; Luciferase; Marker; Membrane; Microscopy; Mutagenesis; Over Expression; Plasmid Preparation; Produced; Purification; Quantitation Assay; Reverse Transcription Polymerase Chain Reaction; Sequencing; Software; Staining; Transfection; Transformation Assay; Western Blot
bioz_score: 94
title: Assessment of SARS-CoV-2 immune escape using antigenic cartography combined with experimental challenge studies
authors: Merel R. te Marvelde; Anna Z. Mykytyn; ...; Bart L. Haagmans
journal: NPJ vaccines
date: 2026-01-02
pmid: 41484114
cited_by_count: 0
techniques: Expressing; Histopathology; Immunohistochemistry; Incubation; Infection; MANN-WHITNEY; Plaque Assay; Quantitative RT-PCR; Software; Staining; Virus
bioz_score: 94
title: Quantum-enhanced nanodiamond rapid test advances early SARS-CoV-2 antigen detection in clinical diagnostics
authors: Alyssa Thomas DeCruz; Benjamin S. Miller; ...; Rachel A. McKendry
journal: Nature Communications
date: 2025-10-02
pmid: 41038811
cited_by_count: 2
techniques: Biomarker Discovery; Chromatin Immunoprecipitation; Flow Cytometry; Magnetic Resonance Imaging
bioz_score: 93
title: Broad-Spectrum Antiviral Styrene Maleic-Acid Copolymer Lipid Particle Nanodiscs for pH-Responsive Irreversible Virus Inactivation
authors: Jaehyeon Hwang; Misoo Kim; ...; Dae-Hyuk Kweon
journal: Biomacromolecules
date: 2025-06-10
pmid: 40493510
cited_by_count: 0
techniques: Incubation
bioz_score: 94
title: Endemic coronavirus infection is associated with SARS-CoV-2 Fc receptor-binding antibodies.
authors: David J Bean; Yan Mei Liang; ...; Manish Sagar
journal: Journal of virology
date: 2025-05-19
pmid: 40387363
cited_by_count: 2
techniques: Binding Assay; Incubation; Magnetic Beads
bioz_score: 94
title: A SARS-CoV-2 Mpro mutation conferring ensitrelvir resistance paradoxically increases nirmatrelvir susceptibility
authors: Min-Suk Song; Seong Cheol Min; ...; Sang Chul Shin
journal: Research Square
date: 2025-04-21
doi: 10.21203/rs.3.rs-6449068/v1
cited_by_count: 0
techniques: Comparison; Histopathology; Immunohistochemical staining; Immunohistochemistry; Recombinant; Software; Staining; Transmission Assay
bioz_score: 94
title: Fc-binding nanodisc restores antiviral efficacy of antibodies with reduced neutralizing effects against evolving SARS-CoV-2 variants.
authors: Jaehyeon Hwang; Soyun Choi; ...; Dae-Hyuk Kweon
journal: Journal of Nanobiotechnology
date: 2025-01-25
pmid: 39856746
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Comparison; Concentration Assay; Control; Electron Microscopy; Enzyme-linked Immunosorbent Assay; Immunohistochemistry; In Vitro; Incubation; Infection; Inhibition; Staining; Standard Deviation; Transgenic Assay; Transmission Assay
bioz_score: 94
title: Viral Mimetic Bacterial Outer Membrane Vesicles for Targeting Angiotensin-Converting Enzyme 2
authors: Gna Ahn; Hyo-Won Yoon; ...; Ji-Young Ahn
journal: International Journal of Nanomedicine
date: 2025-01-16
pmid: 39835181
doi: 10.2147/IJN.S497742
pmcid: 11745048
cited_by_count: 0
techniques: Disruption; Immunohistochemical staining; Software; Staining; Titration
bioz_score: 94
title: Andrographolide attenuates SARS-CoV-2 infection via an up-regulation of glutamate-cysteine ligase catalytic subunit (GCLC).
authors: Jarinya Chaopreecha; Nut Phueakphud; ...; Patompon Wongtrakoongate
journal: Phytomedicine : international journal of phytotherapy and phytopharmacology
date: 2025-01-15
pmid: 39631298
doi: 10.1016/j.phymed.2024.156279
cited_by_count: 2
techniques: Bicinchoninic Acid Protein Assay; Blocking Assay; Detection Assay; Enzyme-linked Immunosorbent Assay; Immunofluorescence; Incubation; MTT Assay; Medications; Reverse Transcription Polymerase Chain Reaction; Western Blot
bioz_score: 94
title: In vitro and in vivo validation of the antiviral effect of hCypA against SARS-CoV-2 via binding to the RBD of spike protein
authors: Woo-Ri Shin; Do-Young Kim; ...; Yang-Hoon Kim
journal: Molecular Therapy
date: 2024-06-05
pmid: 38532628
doi: 10.1016/j.ymthe.2024.03.029
pmcid: 11184304
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Cytokine Assay; Disruption; Expressing; Far Western Blot; Histopathology; Immunofluorescence; Immunohistochemical staining; Immunohistochemistry; In Vivo; Infection; Inhibition; Injection; Ion Exchange Chromatography; Marker; Molecular Weight; Multiplex Assay; Neutralization; Purification; Recombinant; SPR Assay; Saline; Software; Staining; Standard Deviation; Virus
bioz_score: 94
title: Modelling SARS-CoV-2 infection in a human alveolus microphysiological system.
authors: Tanja Šuligoj; Naomi S Coombes; ...; Nathalie Juge
journal: Access Microbiology
date: 2024-03-13
pmid: 39697363
cited_by_count: 1
techniques: Imaging; Immunostaining; Microscopy; Software; Staining
bioz_score: 94
title: Broad-spectrum vaccine via combined immunization routes triggers potent immunity to SARS-CoV-2 and its variants.
authors: Man Xing; Yihan Wang; ...; Dongming Zhou
journal: Journal of virology
date: 2024-01-10
pmid: 37706688
doi: 10.1128/jvi.00724-23
cited_by_count: 9
techniques: Blocking Assay; Immunofluorescence; Incubation; Software; Staining
bioz_score: 94
title: SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell–derived human neurons
authors: Jasmina M. Luczo; Sarah J. Edwards; ...; Vinod Sundaramoorthy
journal: Journal of neurovirology
date: 2024-01-03
pmid: 38172412
doi: 10.1007/s13365-023-01187-3
pmcid: 11035468
cited_by_count: 8
techniques: Imaging; Immunocytochemistry; Immunofluorescence; Incubation; Infection; Marker; Microscopy; Modification; Olfactory; Staining; Virus
bioz_score: 94
title: Single cell spatial analysis reveals inflammatory foci of immature neutrophil and CD8 T cells in COVID-19 lungs
authors: Praveen Weeratunga; Laura Denney; ...; Ling-Pei Ho
journal: Nature Communications
date: 2023-11-08
pmid: 37940670
doi: 10.1038/s41467-023-42421-0
pmcid: 10632491
cited_by_count: 15
techniques: Polymer; Staining
bioz_score: 94
title: SARS-CoV-2 Omicron variant causes brain infection with lymphoid depletion in a mouse COVID-19 model
authors: Na Yun Lee; Youn Woo Lee; ...; Jun Won Park
journal: Laboratory Animal Research
date: 2023-05-09
pmid: 37161442
doi: 10.1186/s42826-023-00157-4
pmcid: 10169124
cited_by_count: 15
techniques: Blocking Assay; Derivative Assay; Histopathology; Imaging; Immunostaining; Incubation; Microscopy; Staining
bioz_score: 94
title: Protocol for in vitro fluorescence assay of papain-like protease and cell-based immunofluorescence assay of coronavirus infection.
authors: JuOae Chang; Jinhee Kim; ...; Wonsik Lee
journal: STAR Protocols
date: 2023-04-24
pmid: 37167057
cited_by_count: 2
bioz_score: 94
title: Unbiased single cell spatial analysis localises inflammatory clusters of immature neutrophils-CD8 T cells to alveolar progenitor cells in fatal COVID-19 lungs
authors: Weeratunga Praveen; Denney Laura; ...; Ho Ling-Pei
journal: medRxiv
date: 2022-12-23
doi: 10.1101/2022.12.21.22283654
cited_by_count: 0
techniques: Derivative Assay; Histopathology; Immunohistochemistry; Staining
bioz_score: 94
title: Hypoxia inducible factors regulate infectious SARS-CoV-2, epithelial damage and respiratory symptoms in a hamster COVID-19 model.
authors: Peter A C Wing; Maria Prange-Barczynska; ...; Jane A McKeating
journal: PLoS Pathogens
date: 2022-09-20
pmid: 36067210
cited_by_count: 19
techniques: Amplification; Hybridization; Immunohistochemistry; Incubation; Polymer; Software; Staining; Virus
bioz_score: 94
title: In vitro and in vivo differences in neurovirulence between D614G, Delta And Omicron BA.1 SARS-CoV-2 variants
authors: Lisa Bauer; Melanie Rissmann; ...; Debby van Riel
journal: Acta Neuropathologica Communications
date: 2022-09-05
pmcid: 9441226
cited_by_count: 23
techniques: Binding Assay; Derivative Assay; Expressing; Flow Cytometry; Immunohistochemistry; In Situ Hybridization; Incubation; Infection; Marker; Plaque Assay; Quantitative RT-PCR; Real-time Polymerase Chain Reaction; Software; Staining; Standard Deviation; Variant Assay
bioz_score: 94
title: Neutrophil Extracellular Traps, Local IL-8 Expression, and Cytotoxic T-Lymphocyte Response in the Lungs of Patients With Fatal COVID-19
authors: Ignacio Melero; María Villalba-Esparza; ...; Carlos E. de Andrea
journal: Chest
date: 2022-06-15
pmid: 35714708
pmcid: 9197577
cited_by_count: 29
techniques: Immunohistochemistry; Infection; Software; Staining; Virus Quantification
bioz_score: 94
title: Spatial transcriptomic characterization of COVID-19 pneumonitis identifies immune circuits related to tissue injury
authors: Cross AR; de Andrea CE; ...; Issa F
journal: bioRxiv
date: 2021-10-25
doi: 10.1101/2021.06.21.449178
cited_by_count: 3
techniques: Polymer; Software; Staining; Virus
bioz_score: 94
title: Antibody persistence and neutralising activity in primary school students and staff: Prospective active surveillance, June to December 2020, England
authors: Georgina Ireland; Anna Jeffery-Smith; ...; Shamez N Ladhani
journal: EClinicalMedicine
date: 2021-09-30
pmid: 34608455
doi: 10.1016/j.eclinm.2021.101150
pmcid: 8481203
cited_by_count: 11
techniques: Binding Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Expressing; Recombinant
bioz_score: 94
title: Interferon-armed RBD dimer enhances the immunogenicity of RBD for sterilizing immunity against SARS-CoV-2
authors: Shiyu Sun; Yueqi Cai; ...; Hua Peng
journal: Cell Research
date: 2021-07-15
pmid: 34267349
doi: 10.1038/s41422-021-00531-8
pmcid: 8280646
cited_by_count: 48
techniques: Activity Assay; Enzyme-linked Immunosorbent Assay; Infection; Injection; Luciferase; Neutralization; Plasmid Preparation; Quantitative RT-PCR; Synthesized
bioz_score: 94
