| Catalog No. | Size | Price (USD) |
| 40143-T62-20 | 20 µL | $118.49 |
| 40143-T62-50 | 50 µL | $222.17 |
| 40143-T62-100 | 100 µL | $370.28 |
General Information
| Product name | SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV Nucleocapsid Has cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# 40588-V08B). |
| Immunogen | Recombinant SARS-CoV Nucleocapsid Protein (Catalog#40143-V08B) |
| Preparation | Produced in rabbits immunized with purified, recombinant SARS-CoV Nucleocapsid (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). SARS-CoV Nucleocapsid specific IgG was purified by SARS-CoV Nucleocapsid affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-SARS-NP rabbit polyclonal antibody at 1:2000 dilution. Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (30ng) Lane B: SARS-CoV NP Protein (Cat#40143-V08B) (5ng) Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (30ng) Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (5ng) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | SARS CoV Nucleocapsid Western blot (WB) 29809 |
| Image 1 URL | Source image |
Background Information
| Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| References |
|
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Nanomedicine","date":"2025-08-06","pmid":"40791772","doi":"10.2147/IJN.S511173"},{"title":"Exposure to two antigenically distinct SARS-CoV-2 variants broadens neutralization patterns.","authors":["Melanie M Schmitt","Annika Rössler","...","Janine Kimpel"],"journal":"Vaccine","date":"2025-07-04","pmid":"40617091","doi":"10.1016/j.vaccine.2025.127459"},{"title":"Dominant and cross-reactive S1-specific memory B cell response induced by primary SARS-CoV-2 infection","authors":["Xiaomin Xing","Zhaoyong Zhang","...","Lei Yu"],"journal":"Scientific Reports","date":"2025-07-01","pmid":"40593005","doi":"10.1038/s41598-025-06847-4"},{"title":"Papain-Like Protease of SARS-CoV-2 Induces Intestinal Inflammation via the ISG15 Pathway: Identification of Natural Compound Inhibitors.","authors":["Fang Wu","Zhaoyong Zhang","...","Wei Xu"],"journal":"Journal of medical virology","date":"2025-06-16","pmid":"40522277","doi":"10.1002/jmv.70448"},{"title":"β-Coronaviruses exploit ESCRT for virion assembly 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Breugem","Samra Riesebosch","...","Bart L. Haagmans"],"journal":"npj Viruses","date":"2024-09-02","pmid":"","doi":"10.1038/s44298-024-00054-0"},{"title":"The histamine receptor H1 acts as an alternative receptor for SARS-CoV-2","authors":["Fei Yu","Xiaoqing Liu","...","Chunfu Zheng"],"journal":"mBio","date":"2024-08-01","pmid":"38953634","doi":""},{"title":"IL-13 decreases susceptibility to airway epithelial SARS-CoV-2 infection but increases disease severity in vivo","authors":["Ghimire Shreya","Xue Biyun","...","Pezzulo Alejandro A."],"journal":"bioRxiv","date":"2024-07-04","pmid":"","doi":""},{"title":"SARS-CoV-2 infection induces adaptive NK cell responses by spike protein-mediated induction of HLA-E expression.","authors":["Mohammad Zahidul Hasan","Maren Claus","...","Lutz Walter"],"journal":"Emerging microbes & infections","date":"2024-06-27","pmid":"38804979","doi":""},{"title":"Human angiotensin converting enzyme 2 specific antisense oligonucleotides reduce infection with SARS-CoV-2 variants.","authors":["Tong Lu","Chengcheng Zhang","...","Weiping Wen"],"journal":"The Journal of allergy and clinical immunology","date":"2024-06-21","pmid":"38909634","doi":"10.1016/j.jaci.2024.06.007"},{"title":"A predominately pulmonary activation of complement in a mouse model of severe COVID-19","authors":["Szachowicz Peter J.","Wohlford-Lenane Christine","...","McCray Paul B. Jr."],"journal":"bioRxiv","date":"2024-06-03","pmid":"","doi":""},{"title":"Mucoadhesive film for oral delivery of vaccines for protection of the respiratory tract.","authors":["Hana Esih","Klemen Mezgec","...","Roman Jerala"],"journal":"Journal of controlled release : official journal of the Controlled Release Society","date":"2024-05-29","pmid":"38795814","doi":"10.1016/j.jconrel.2024.05.041"},{"title":"Direct comparison of SARS-CoV-2 variant specific neutralizing antibodies in human and hamster sera.","authors":["Annika Rössler","Antonia Netzl","...","Janine Kimpel"],"journal":"NPJ vaccines","date":"2024-05-18","pmid":"38762525","doi":""},{"title":"Original COVID-19 priming regimen impacts the immunogenicity of bivalent BA.1 and BA.5 boosters.","authors":["Luca M Zaeck","Ngoc H Tan","...","Rory D de Vries"],"journal":"Nature Communications","date":"2024-05-18","pmid":"38762522","doi":""},{"title":"SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson’s disease in human dopaminergic neurons and a mouse model","authors":["Bina Lee","Ha Nyeoung Choi","...","Seung Pil Yun"],"journal":"Cell Reports Medicine","date":"2024-05-14","pmid":"38749422","doi":""},{"title":"Discovery of natural catechol derivatives as covalent SARS-CoV-2 3CL pro inhibitors.","authors":["Feng Wang","Donglan Liu","...","Ping Tian"],"journal":"International Journal of Biological Macromolecules","date":"2024-04-08","pmid":"38395279","doi":"10.1016/j.ijbiomac.2024.130377"},{"title":"Viral presence and immunopathology in a kidney transplant recipient with fatal COVID-19: a clinical autopsy report.","authors":["Larissa E van Eijk","Arno R Bourgonje","...","Jan-Luuk Hillebrands"],"journal":"Journal of leukocyte biology","date":"2024-04-01","pmid":"38252562","doi":"10.1093/jleuko/qiae016"},{"title":"TRIM6 facilitates SARS-CoV-2 proliferation by catalyzing the K29-typed ubiquitination of NP to enhance the ability to bind viral genomes.","authors":["Jian Zhou","Yuzheng Zhou","...","Zheng Zhang"],"journal":"Journal of medical virology","date":"2024-03-25","pmid":"38515377","doi":"10.1002/jmv.29531"},{"title":"SARS-CoV-2 Neutralization Capacity in Hemodialysis Patients with and without a Fifth Vaccination with the Updated Comirnaty Original/Omicron BA.4-5 Vaccine","authors":["Bo-Hung Liao","Louise Platen","...","Gianni Gori Savellini"],"journal":"Vaccines","date":"2024-03-15","pmid":"38543942","doi":"10.3390/vaccines12030308"},{"title":"Cross-protective HCoV immunity reduces symptom development during SARS-CoV-2 infection","authors":["Irene A. Abela","Magdalena Schwarzmüller","...","Satya Dandekar"],"journal":"mBio","date":"2024-02-01","pmid":"38270455","doi":"10.1128/mbio.02722-23"},{"title":"Identification of a broad sarbecovirus neutralizing antibody targeting a conserved epitope on the receptor-binding domain.","authors":["Yanqun Wang","Zhaoyong Zhang","...","Jincun Zhao"],"journal":"Cell Reports","date":"2024-01-03","pmid":"38175758","doi":"10.1016/j.celrep.2023.113653"},{"title":"Oral delivery of a chitosan adjuvanted COVID-19 vaccine provides long-lasting and broad-spectrum protection against SARS-CoV-2 variants of concern in golden hamsters.","authors":["Shen Wang","Huan Cui","...","Xianzhu Xia"],"journal":"Antiviral research","date":"2023-12-16","pmid":"38036065","doi":"10.1016/j.antiviral.2023.105765"},{"title":"Ancestral, Delta, and Omicron (BA.1) SARS-CoV-2 strains are dependent on serine proteases for entry throughout the human respiratory tract.","authors":["Matthew J Gartner","Leo Yi Yang Lee","...","Kanta Subbarao"],"journal":"Med (New York, N.Y.)","date":"2023-12-16","pmid":"37769654","doi":"10.1016/j.medj.2023.08.006"},{"title":"Dynamic immune landscape in vaccinated-BA.5-XBB.1.9.1 reinfections revealed a 5-month protection-duration against XBB infection and a shift in immune imprinting.","authors":["Tingting Cui","Xiaoling Su","...","Zhongfang Wang"],"journal":"EBioMedicine","date":"2023-12-07","pmid":"38064992","doi":""},{"title":"Longitudinal SARS-CoV-2 neutralization of Omicron BA.1, BA.5 and BQ.1.1 after four vaccinations and the impact of breakthrough infections in haemodialysis patients","authors":["Louise Platen","Bo-Hung Liao","...","Ulrike Protzer"],"journal":"Clinical Kidney Journal","date":"2023-12-01","pmid":"","doi":"10.1093/ckj/sfad147"},{"title":"A potent and broad-spectrum neutralizing nanobody for SARS-CoV-2 viruses, including all major Omicron strains.","authors":["Hebang Yao","Hongyang Wang","...","Wenqing Xu"],"journal":"MedComm","date":"2023-12-01","pmid":"37901798","doi":""},{"title":"Luminescent reporter cells enable the identification of broad-spectrum antivirals against emerging viruses.","authors":["Karin Löw","Rebecca Möller","...","Gisa Gerold"],"journal":"Journal of medical virology","date":"2023-11-27","pmid":"37975336","doi":"10.1002/jmv.29211"},{"title":"A Naïve Phage Display Library-Derived Nanobody Neutralizes SARS-CoV-2 and Three Variants of Concern","authors":["Dandan Wu","Junxiao Cong","...","Liang Ye"],"journal":"International Journal of Nanomedicine","date":"2023-10-16","pmid":"","doi":"10.2147/IJN.S427990"},{"title":"Cross-protection and cross-neutralization capacity of ancestral and VOC-matched SARS-CoV-2 adenoviral vector-based vaccines","authors":["Sabrina E. Vinzón","María V. Lopez","...","Osvaldo L. Podhajcer"],"journal":"NPJ vaccines","date":"2023-10-04","pmid":"37794010","doi":""},{"title":"SARS-CoV-2 N protein-induced Dicer, XPO5, SRSF3, and hnRNPA3 downregulation causes pneumonia","authors":["Luo Yu-Wei","Zhou Jiang-Peng","...","Tang Kai-Fu"],"journal":"bioRxiv","date":"2023-10-04","pmid":"","doi":"10.1101/2023.10.03.560426"},{"title":"SARS-CoV-2 neurotropism-induced anxiety and depression-like behaviors require Microglia activation","authors":["Ge Qian","Zhou Shan","...","Li Kun"],"journal":"bioRxiv","date":"2023-10-03","pmid":"","doi":""},{"title":"Discovery of highly potent covalent SARS-CoV-2 3CL pro inhibitors bearing 2-sulfoxyl-1,3,4-oxadiazole scaffold for combating COVID-19.","authors":["Fu-Mao Zhang","Ting Huang","...","Ping Tian"],"journal":"European Journal of Medicinal Chemistry","date":"2023-09-19","pmid":"37598484","doi":"10.1016/j.ejmech.2023.115721"},{"title":"A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung.","authors":["Woochan Lee","Seyoon Lee","...","Jong-Il Kim"],"journal":"Experimental & molecular medicine","date":"2023-09-04","pmid":"37582976","doi":""},{"title":"Glycosylated extracellular mucin domains protect against SARS-CoV-2 infection at the respiratory surface.","authors":["Maitrayee Chatterjee","Liane Z X Huang","...","Karin Strijbis"],"journal":"PLoS Pathogens","date":"2023-08-31","pmid":"37561789","doi":""},{"title":"Broadly Effective ACE2 Decoy Proteins Protect Mice from Lethal SARS-CoV-2 Infection.","authors":["Mengjia Lu","Weitong Yao","...","Guocai Zhong"],"journal":"Microbiology Spectrum","date":"2023-08-21","pmid":"37395664","doi":"10.1128/spectrum.01100-23"},{"title":"Acute bone loss following SARS-CoV-2 infection in mice.","authors":["Anne K Haudenschild","Blaine A Christiansen","...","Dominik R Haudenschild"],"journal":"Journal of orthopaedic research : official publication of the Orthopaedic Research Society","date":"2023-08-04","pmid":"36815216","doi":"10.1002/jor.25537"},{"title":"Evaluating Z-FA-FMK, a host cathepsin L protease inhibitor, as a potent and broad-spectrum antiviral therapy against SARS-CoV-2 and related coronaviruses.","authors":["Ju Hwan Jeong","Jang-Hoon Choi","...","Min-Suk Song"],"journal":"Antiviral research","date":"2023-08-01","pmid":"37437781","doi":"10.1016/j.antiviral.2023.105669"},{"title":"Immunogenicity and efficacy of a novel multi-patch SARS-CoV-2/COVID-19 vaccine candidate.","authors":["Beatriz Perdiguero","Laura Marcos-Villar","...","Carmen Elena Gómez"],"journal":"Frontiers in Immunology","date":"2023-07-07","pmid":"37404819","doi":"10.3389/fimmu.2023.1160065"},{"title":"Pseudoknot-targeting Cas13b combats SARS-CoV-2 infection by suppressing viral replication.","authors":["Daseuli Yu","Hee-Jeong Han","...","Won Do Heo"],"journal":"Molecular therapy : the journal of the American Society of Gene Therapy","date":"2023-06-12","pmid":"36945774","doi":"10.1016/j.ymthe.2023.03.018"},{"title":"Sequential Glycosylations at the Multibasic Cleavage Site of SARS-CoV-2 Spike Protein Regulate Viral Activation, Assembly, and Infection","authors":["Shengjun Wang","Wei Ran","...","Yang Mao"],"journal":"Europe PMC","date":"2023-06-08","pmid":"","doi":"10.21203/rs.3.rs-2703199"},{"title":"Technical note on the exploration of COVID-19 in autopsy material","authors":["Matthew Phillip Humphries","Victoria Bingham","...","Manuel Salto-Tellez"],"journal":"Journal of clinical pathology","date":"2023-06-01","pmid":"36717223","doi":""},{"title":"SARS-CoV-2 awakens ancient retroviral genes and the expression of proinflammatory HERV-W envelope protein in COVID-19 patients.","authors":["Benjamin Charvet","Joanna Brunel","...","Branka Horvat"],"journal":"iScience","date":"2023-05-19","pmid":"37091988","doi":"10.1016/j.isci.2023.106604"},{"title":"Defining distinct RNA-protein interactomes of SARS-CoV-2 genomic and subgenomic RNAs","authors":["Isabella T. Whitworth","Rachel A. Knoener","...","Nathan M. 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Martínez-Colón","Kalani Ratnasiri","...","Tracey L. McLaughlin"],"journal":"Science translational medicine","date":"2022-09-22","pmid":"36137009","doi":"10.1126/scitranslmed.abm9151"},{"title":"In vitro and in vivo differences in neurovirulence between D614G, Delta And Omicron BA.1 SARS-CoV-2 variants","authors":["Lisa Bauer","Melanie Rissmann","...","Debby van Riel"],"journal":"Acta Neuropathologica Communications","date":"2022-09-05","pmid":"","doi":""},{"title":"Improved SARS-CoV-2 Neutralization of Delta and Omicron BA.1 Variants of Concern after Fourth Vaccination in Hemodialysis Patients.","authors":["Cho-Chin Cheng","Louise Platen","...","Matthias Christoph Braunisch"],"journal":"Vaccines","date":"2022-08-16","pmid":"36016216","doi":"10.3390/vaccines10081328"}]
