| Catalog No. | Size | Price (USD) |
| 40588-MM170-20 | 20 µL | $296.22 |
| 40588-MM170-100 | 100 µL | $592.44 |
General Information
| Product name | SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), LFA(Cap) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Nucleocapsid Has cross-reactivity in WB and ELISA with SARS-COV-2 Omicron (JN.1/BA.2.86) Nucleocapsid Protein (Cat# 40588-V07E42) SARS-COV-2 Omicron (BF.7) Nucleocapsid Protein (Cat# 40588-V07E40) SARS-COV-2 Omicron (BQ.1/BQ.1.1) Nucleocapsid Protein (Cat# 40588-V07E39) SARS-CoV-2 Omicron (BA.4) Nucleocapsid Protein (Cat# 40588-V07E37) SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein (Cat# 40588-V07E34) SARS-CoV-2 Nucleocapsid (P80R) Protein (Cat# 40588-V07E11) SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) Protein (Cat# 40588-V07E7) SARS-CoV-2 Nucleocapsid Protein (Cat# 40588-V08B) Has cross-reactivity in WB with SARS-CoV-2 Nucleocapsid (T205I) Protein (Cat# 40588-V07E9) No cross-reactivity in WB and ELISA with SARS-CoV-2 Nucleocapsid NTD Protein (Cat# 40588-V07E10) SARS-CoV-2 Nucleocapsid CTD Protein (Cat# 40588-V07E5) No cross-reactivity in ELISA with SARS-CoV-2 Nucleocapsid (T205I) Protein (Cat# 40588-V07E9) |
| Immunogen | Recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog# 40588-V08B) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 Nucleocapsid (Catalog#40588-V08B; YP_009724397.2(335Gly/Ala); Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #170 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid mouse monoclonal antibody in 1 μg/mL. Sample: Recombinant Protein 10 ng Lane 1: SARS-COV-2 JN.1/BA.2.86 (Omicron) Nucleoprotein/NP Protein (Cat#40588-V07E42) Lane 2: SARS-COV-2 BQ.1/BQ.1.1 (Omicron) Nucleocapsid Protein (Cat#40588-V07E39) Lane 3: SARS-COV-2 BF.7 (Omicron) Nucleocapsid Protein (Cat#40588-V07E40) Lane 4: SARS-CoV-2 (BA.4) Nucleocapsid Protein (Cat#40588-V07E37) Lane 5: SARS-CoV-2 Nucleocapsid (P80R) Protein (Cat#40588-V07E11) Lane 6: SARS-CoV-2 Nucleocapsid (T205I) Protein (Cat#40588-V07E9) Lane 7: SARS-CoV-2 Nucleocapsid NTD Protein (Cat#40588-V07E10) Lane 8: SARS-CoV-2 Nucleocapsid CTD Protein (Cat#40588-V07E5) Lane 9: SARS-CoV-2 Nucleocapsid-His recombinant Protein (Cat#40588-V08B) Lane 10: SARS-CoV-2 Nucleocapsid(D3L, R203K, G204R, S235F)-His Recombinant Protein (Cat#40588-V07E7) Lane 11: SARS-CoV-2 B.1.1.529 (Omicron) Nucleocapsid Protein (Cat#40588-V07E34) Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | SARS-CoV-2 Coronavirus Nucleocapsid Western blot (WB) 43049 |
| Image 1 URL | Source image |
Background Information
| Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| References |
|
title: Anti-SARS-CoV-2 antibodies from severe COVID-19 individuals or S2 immunizations do not worsen disease in hamsters
authors: Núria Pedreño-López; Ferran Tarrés-Freixas; ...; Jorge Carrillo
journal: Communications Biology
date: 2025-11-24
pmid: 41286076
cited_by_count: 1
techniques: Activation Assay; Clinical Proteomics; Incubation; Magnetic Beads
bioz_score: 93
title: Difference in levels of SARS-CoV-2 S1 and S2 subunits- and nucleocapsid protein-reactive SIgM/IgM, IgG and SIgA/IgA antibodies in human milk
authors: Veronique Demers-Mathieu; Dung M. Do; ...; Elena Medo
journal: Journal of Perinatology
date: 2020-09-01
pmid: 32873904
doi: 10.1038/s41372-020-00805-w
pmcid: 7461757
cited_by_count: 61
techniques: Blocking Assay; Diagnostic Assay; Enzyme-linked Immunosorbent Assay; Incubation; Recombinant
bioz_score: 93
