| Catalog No. | Size | Price (USD) |
| 40588-R0004-20 | 20 µL | $296.22 |
| 40588-R0004-100 | 100 µL | $592.44 |
General Information
| Product name | SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Nucleocapsid Has cross-reactivity in ELISA and WB with MERS-CoV Nucleocapsid Protein (Cat# 40068-V08B) Has cross-reactivity in ELISA with SARS-CoV Nucleocapsid Protein (Cat# 40143-V08B) Minimal cross-reactivity in ELISA with SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein (Cat #40588-V07E34) SARS-CoV-2 Omicron (BA.2) Nucleocapsid Protein (Cat# 40588-V07E35) SARS-CoV-2 Omicron XE(BA.1 x BA.2) Nucleocapsid Protein (Cat# 40588-V07E36) SARS-CoV-2 Omicron (BA.4) Nucleocapsid Protein (Cat# 40588-V07E37) No cross-reactivity in ELISA with SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein (Cat# 40588-V07E34) HCoV-229E Nucleocapsid protein (Cat# 40640-V07E) HCoV-NL63 Nucleocapsid protein (Cat# 40641-V07E) HCoV-HKU1(isolate N5) Nucleocapsid protein (Cat# 40642-V07E) HCoV-OC43 Nucleocapsid protein (Cat# 40643-V07E) Influenza A H1N1 (A/California/07/2009) Nucleoprotein Protein (Cat# 40205-V08B) Influenza A H3N2 (A/Switzerland/9715293/2013) Nucleoprotein Protein (Cat# 40499-V08B) Influenza A H2N2 (A/Ann Arbor/6/1960) Nucleoprotein Protein (Cat# 40033-V08B) Influenza A H7N9 (A/Shanghai/2/2013) Nucleoprotein Protein (Cat# 40111-V08B) Influenza B (B/Florida/4/2006) Nucleoprotein Protein (Cat# 40438-V08B) |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Catalog#40588-V08B) |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Source | Monoclonal Rabbit IgG Clone #0004 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-SARS-NP rabbit monoclonal antibody at 1:1000 dilution. Lane A: SARS-CoV-2 (2019-nCoV) Nucleocapsid (40588-V08B) (30ng) Lane B: MERS-CoV Nucleocapsid (40068-V08B) (30ng) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | 2019-nCoV Coronavirus Nucleocapsid Western blot (WB) 30100 |
| Image 1 URL | Source image |
Background Information
| Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| References |
|
title: Development of multivalent SARS-CoV-2 virus-like particle vaccine candidates.
authors: Urban Bezeljak; Alexander Jerman; ...; Matjaž Peterka
journal: Vaccine
date: 2025-06-14
pmid: 40517501
doi: 10.1016/j.vaccine.2025.127394
cited_by_count: 1
techniques: Membrane; Polyacrylamide Gel Electrophoresis; Positive Control; Recombinant; SDS Page; Staining; Western Blot
bioz_score: 93
title: Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity
authors: Minghui Yang; Chunhui Li; ...; Yongxiang Zhao
journal: Signal transduction and targeted therapy
date: 2024-02-14
pmid: 38355661
doi: 10.1038/s41392-024-01748-w
pmcid: 10866937
cited_by_count: 13
techniques: Cell Counting; Luciferase; Lysis; Polymerase Chain Reaction
bioz_score: 93
title: Direct capture and smartphone quantification of airborne SARS-CoV-2 on a paper microfluidic chip
authors: Sangsik Kim; Patarajarin Akarapipad; ...; Jeong-Yeol Yoon
journal: Biosensors & bioelectronics
date: 2021-12-24
pmid: 34973565
doi: 10.1016/j.bios.2021.113912
pmcid: 8701770
cited_by_count: 34
techniques: Concentration Assay; Fluorescence; Generated; Infection
bioz_score: 93
title: Highly sensitive and ultra-rapid antigen-based detection of SARS-CoV-2 using nanomechanical sensor platform
authors: Dilip Kumar Agarwal; Vikas Nandwana; ...; Vinayak P. Dravid
journal: Biosensors & bioelectronics
date: 2021-09-17
pmid: 34583103
doi: 10.1016/j.bios.2021.113647
pmcid: 8445766
cited_by_count: 49
techniques: Recombinant
bioz_score: 93
title: Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein
authors: Curtis D. Hodge; Daniel. J. Rosenberg; ...; Michal Hammel
journal: mAbs
date: 2021-04-12
pmid: 33843452
pmcid: 8043170
cited_by_count: 15
bioz_score: 93
