| Catalog No. | Size | Price (USD) |
| 40588-RA84-20 | 20 µL | $296.22 |
| 40588-RA84-100 | 100 µL | $592.44 |
General Information
| Product name | SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), ELISA(Det) (FAQ Protocol), IHC-P (FAQ Protocol), IP (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Nucleocapsid Has cross-reactivity in ELISA with SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein (Cat #40588-V07E34) SARS-CoV-2 Omicron (BA.2) Nucleocapsid Protein (Cat# 40588-V07E35) SARS-CoV-2 Omicron XE(BA.1 x BA.2) Nucleocapsid Protein (Cat# 40588-V07E36) SARS-CoV-2 Omicron (BA.4) Nucleocapsid Protein (Cat# 40588-V07E37) SARS-CoV-2 Delta (B.1.617.2) Nucleocapsid Protein (Cat# 40588-V07E29) SARS-CoV-2 Nucleocapsid Protein (Cat# 40588-V08B) SARS-CoV Nucleocapsid Protein (Cat# 40143-V08B) No cross-reactivity in ELISA with MERS-CoV Nucleocapsid protein (Cat# 40068-V08B) HCoV-229E Nucleocapsid Protein (Cat# 40640-V07E) HCoV-HKU1 Nucleocapsid Protein (Cat# 40642-V07E) HCoV-NL63 Nucleocapsid Protein (Cat# 40641-V07E) HCoV-OC43 Nucleocapsid Protein (Cat# 40643-V07E) H1N1 (A/California/07/2009) NP Protein (Cat# 40205-V08B) H2N2 (A/Ann Arbor/6/1960) NP Protein (Cat# 40033-V08B) H3N2 (A/Switzerland/9715293/2013) NP Protein (Cat# 40499-V08B) H7N9 (A/Shanghai/2/2013) NP Protein (Cat# 40111-V08B) Influenza B (B/Florida/4/2006) NP Protein (Cat# 40438-V08B) |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Catalog#40588-V08B) |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Source | Monoclonal Rabbit IgG Clone #A84 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid rabbit monoclonal antibody at 1:5000 dilution. Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. (Validation Experiment) |
| Image 1 Alt Text | SARS-CoV-2 Coronavirus Nucleocapsid Western blot (WB) 32579 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid rabbit monoclonal antibody at 1:5000 dilution. Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. (Validation Experiment) |
| Image 2 Alt Text | SARS-CoV-2 Coronavirus Nucleocapsid Immunohistochemistry(IHC) 32679 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid rabbit monoclonal antibody at 1:5000 dilution. Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. (Validation Experiment) |
| Image 3 Alt Text | SARS-CoV-2 Coronavirus Nucleocapsid Immunoprecipitation(IP) 33701 |
| Image 3 URL | Source image |
Background Information
| Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| References |
|
title: Preclinical evaluation of a multi-epitope mRNA vaccine platform for broad and durable SARS-CoV-2 protection
authors: Laura Marcos-Villar; Beatriz Perdiguero; ...; Carmen Elena Gómez
journal: Frontiers in Immunology
date: 2026-05-05
pmid: 42164511
doi: 10.3389/fimmu.2026.1787877
cited_by_count: 0
techniques: Clear Native PAGE; Confocal Microscopy; Derivative Assay; Electrophoresis; Expressing; In Vitro; Labeling; Membrane; Staining; Transfection; Western Blot
bioz_score: 94
