| Catalog No. | Size | Price (USD) |
| 40069-RP01-20 | 20 µL | $88.87 |
| 40069-RP01-100 | 100 µL | $251.64 |
| 40069-RP01-200 | 200 µL | $370.28 |
General Information
| Product name | MERS-CoV Spike Antibody, Rabbit PAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | MERS-CoV (NCoV / Novel coronavirus) Spike |
| Immunogen | Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297) (Catalog#40069-V08B) |
| Preparation | Produced in rabbits immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (Catalog#40069-V08B; AFS88936.1; Met1-Trp1297). Total IgG was purified by Protein A affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: A single-dose intranasal immunization with a novel bat influenza A virus-vectored MERS vaccine provides effective protection against lethal MERS-CoV challenge
authors: Lei Shi; Sawrab Roy; ...; Xiang-Jin Meng
journal: mBio
date: 2025-08-01
pmid: 40586550
doi: 10.1128/mbio.01107-25
cited_by_count: 1
techniques: Cell Culture; Enzyme-linked Immunosorbent Assay; Enzyme-linked Immunospot; Expressing; Immunofluorescence; Immunohistochemistry; Immunopeptidomics; Incubation; Infection; Plasmid Preparation; Purification; Recombinant; Software; Staining; Titration; Transgenic Assay; Virus; Western Blot
bioz_score: 93
title: Interleukin-1 prevents SARS-CoV-2-induced membrane fusion to restrict viral transmission via induction of actin bundles
authors: Xu Zheng; Shi Yu; ...; John W Schoggins
journal: eLife
date: 2025-02-12
doi: 10.7554/eLife.98593
pmcid: 11820142
cited_by_count: 0
techniques: Activation Assay; Enzyme-linked Immunosorbent Assay; In Vitro; In Vivo; Plasmid Preparation; Purification; Recombinant; Sequencing; Western Blot
bioz_score: 93
title: Coronavirus nucleocapsid-based vaccine provides partial protection against hetero-species coronavirus in murine models.
authors: Pureum Lee; Jihee Kim; ...; Doo-Jin Kim
journal: Antiviral research
date: 2024-11-10
pmid: 39181216
doi: 10.1016/j.antiviral.2024.105991
cited_by_count: 1
techniques: Incubation; Polyacrylamide Gel Electrophoresis; Saline; Western Blot
bioz_score: 93
title: Resistance to SARS-CoV-2 infection in camelid nasal organoids is associated with lack of ACE2 expression
authors: Tim I. Breugem; Samra Riesebosch; ...; Bart L. Haagmans
journal: npj Viruses
date: 2024-09-02
doi: 10.1038/s44298-024-00054-0
pmcid: 11721443
cited_by_count: 0
techniques: Blocking Assay; Imaging; Immunohistochemistry; Incubation; Infection; Microscopy; Plaque Assay; Quantitative RT-PCR; Software; Staining; Titration
bioz_score: 93
title: Hyperforin, the major metabolite of St. John’s wort, exhibits pan-coronavirus antiviral activity
authors: Imelda Raczkiewicz; Céline Rivière; ...; Karin Séron
journal: Frontiers in Microbiology
date: 2024-08-08
doi: 10.3389/fmicb.2024.1443183
pmcid: 11339956
cited_by_count: 0
bioz_score: 93
title: Major Metabolites from Hypericum Perforatum L., Hyperforin and Hypericin, are both active against Human Coronaviruses
authors: Raczkiewicz I.; Rivière C.; ...; Séron K.
journal: bioRxiv
date: 2024-04-13
doi: 10.1101/2024.04.09.588755
cited_by_count: 0
techniques: Infection; MTS Assay; Staining; Titration; Variant Assay; Western Blot
bioz_score: 93
title: Disinfection Effect of Hexadecyl Pyridinium Chloride on SARS-CoV-2 in vitro.
authors: Ke-da Chen; Fei-Ke Ma; ...; Yan-Jun Zhang
journal: Intervirology
date: 2023-12-22
pmid: 36103866
doi: 10.1159/000526241
cited_by_count: 3
techniques: Staining
bioz_score: 93
title: The KxGxYR and DxE motifs in the C-tail of the Middle East respiratory syndrome coronavirus membrane protein are crucial for infectious virus assembly.
authors: Lowiese Desmarets; Adeline Danneels; ...; Sandrine Belouzard
journal: Cellular and Molecular Life Sciences: CMLS
date: 2023-11-10
pmid: 37940699
doi: 10.1007/s00018-023-05008-y
cited_by_count: 4
techniques: Incubation; Membrane; Western Blot
bioz_score: 93
title: Immunogenicity of mucosal COVID-19 vaccine candidates based on the highly attenuated vesicular stomatitis virus vector (VSV MT ) in golden syrian hamster
authors: Yong Ke; En Zhang; ...; Baohong Zhang
journal: Acta Pharmaceutica Sinica. B
date: 2023-08-25
doi: 10.1016/j.apsb.2023.08.023
pmcid: 10692390
cited_by_count: 6
techniques: Binding Assay; Clone Assay; Construct; Expressing; Flow Cytometry; Fluorescence; Incubation; Infection; Microscopy; Mutagenesis; Plasmid Preparation; Recombinant; Sequencing; Staining; Transfection; Virus; Western Blot
bioz_score: 93
title: Susceptibility to SARS-CoV-2 and MERS-CoV in Beagle Dogs.
authors: Kwang-Soo Lyoo; Yoon-Hwan Yeo; ...; Daesub Song
journal: Animals : An Open Access Journal From Mdpi
date: 2023-02-10
pmid: 36830411
cited_by_count: 2
techniques: Histopathology; Incubation; Microscopy
bioz_score: 94
title: Nanocell COVID-19 vaccine triggers a novel immune response pathway producing high-affinity antibodies which neutralize all variants of concern
authors: Steven Y. Gao; Nancy B. Amaro-Mugridge; ...; Himanshu Brahmbhatt
journal: Frontiers in Immunology
date: 2023-01-27
doi: 10.3389/fimmu.2022.1038562
pmcid: 9929940
cited_by_count: 0
techniques: Blocking Assay; DC Protein Assay; Enzyme-linked Immunosorbent Assay; Generated; Incubation; Serial Dilution
bioz_score: 93
title: Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics
authors: Patrick Vogel; Martin Andreas Rückert; ...; Volker Christian Behr
journal: Nature Communications
date: 2022-11-24
doi: 10.1038/s41467-022-34941-y
pmcid: 9700695
cited_by_count: 0
techniques: Affinity Purification; Expressing; Modification
bioz_score: 93
title: UBR5 Acts as an Antiviral Host Factor against MERS-CoV via Promoting Ubiquitination and Degradation of ORF4b.
authors: Yuzheng Zhou; Rong Zheng; ...; Zanxian Xia
journal: Journal of virology
date: 2022-09-16
pmid: 35980206
doi: 10.1128/jvi.00741-22
cited_by_count: 7
techniques: Confocal Microscopy; Incubation; Staining
bioz_score: 94
title: Hydrophobic Alpha-Helical Short Peptides in Overlapping Reading Frames of the Coronavirus Genome
authors: Takashi Okura; Kazuya Shirato; ...; Sonia Zuñiga
journal: Pathogens
date: 2022-08-03
pmid: 36014999
doi: 10.3390/pathogens11080877
pmcid: 9415614
cited_by_count: 0
techniques: Blocking Assay; Expressing; Immunofluorescence; Incubation; Microscopy; Staining; Transfection
bioz_score: 93
title: Nanocell COVID-19 vaccine elicits iNKT-licensed dendritic cells to produce high affinity antibodies neutralizing Variants of Concern
authors: Steven Gao; Nancy Amaro-Mugridge; ...; Himanshu Brahmbhatt
journal: Europe PMC
date: 2022-04-07
doi: 10.21203/rs.3.rs-1472661
cited_by_count: 0
techniques: Blocking Assay; DC Protein Assay; Generated; Incubation; Serial Dilution
bioz_score: 93
title: Inactivated Rabies Virus Vectored MERS-Coronavirus Vaccine Induces Protective Immunity in Mice, Camels, and Alpacas
authors: Hang Chi; Yanqun Wang; ...; Xianzhu Xia
journal: Frontiers in Immunology
date: 2022-01-31
doi: 10.3389/fimmu.2022.823949
pmcid: 8842186
cited_by_count: 4
techniques: Immunofluorescence; Incubation; Microscopy; Staining
bioz_score: 93
title: Broad Spectrum Antiviral Properties of Cardiotonic Steroids Used as Potential Therapeutics for Emerging Coronavirus Infections
authors: Young-Hee Jin; Sangeun Jeon; ...; Maria Rosaria Lauro
journal: Pharmaceutics
date: 2021-11-02
pmid: 34834252
doi: 10.3390/pharmaceutics13111839
pmcid: 8618917
cited_by_count: 18
techniques: Activity Assay; Expressing; Imaging; Immunofluorescence; Infection; Inhibition; Selection; Software; Staining
bioz_score: 91
title: Scanning Electron Microscopic Findings on Respiratory Organs of Some Naturally Infected Dromedary Camels with the Lineage-B of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Saudi Arabia—2018
authors: Abdelmohsen Alnaeem; Samy Kasem; ...; Marc Desforges
journal: Pathogens
date: 2021-04-01
pmid: 33916036
doi: 10.3390/pathogens10040420
pmcid: 8065699
cited_by_count: 3
techniques: Immunohistochemistry
bioz_score: 93
title: Some pathological observations on the naturally infected dromedary camels (Camelus dromedarius) with the Middle East respiratory syndrome coronavirus (MERS-CoV) in Saudi Arabia 2018–2019
authors: Abdelmohsen Alnaeem; Samy Kasem; ...; Maged Gomaa Hemida
journal: The Veterinary Quarterly
date: 2020-07-03
pmid: 32543343
doi: 10.1080/01652176.2020.1781350
pmcid: 7734115
cited_by_count: 11
techniques: Immunohistochemistry
bioz_score: 93
title: Ca 2+ Ions Promote Fusion of Middle East Respiratory Syndrome Coronavirus with Host Cells and Increase Infectivity
authors: Marco R. Straus; Tiffany Tang; ...; Gary R. Whittaker
journal: Journal of virology
date: 2020-07-01
pmid: 32295925
doi: 10.1128/JVI.00426-20
pmcid: 7307142
cited_by_count: 64
techniques: Activity Assay; Binding Assay; Blocking Assay; Concentration Assay; Construct; Expressing; Immunofluorescence; Infection; Isolation; Labeling; Luciferase; Microscopy; Mutagenesis; Plasmid Preparation; Protease Inhibitor; SDS Page; Sequencing; Staining; Transfection; Western Blot
bioz_score: 90
title: Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity
authors: Bryan A. Johnson; Adam Hage; ...; Vineet D. Menachery
journal: Journal of virology
date: 2019-11-15
doi: 10.1128/jvi.01282-19
cited_by_count: 43
techniques: Electron Microscopy; Flow Cytometry; Imaging; Incubation; Purification; Recombinant; Transmission Assay
bioz_score: 94
title: Structural Definition of a Neutralization-Sensitive Epitope on the MERS-CoV S1-NTD
authors: Nianshuang Wang; Osnat Rosen; ...; Jason S. McLellan
journal: Cell Reports
date: 2019-09-24
pmid: 31553909
doi: 10.1016/j.celrep.2019.08.052
pmcid: 6935267
cited_by_count: 64
techniques: Activity Assay; Binding Assay; Competitive Binding Assay; Concentration Assay; Cryo-EM Sample Prep; Expressing; Fluorescence; Generated; Incubation; Inhibition; Labeling; Luciferase; Negative Control; Neutralization; Plasmid Preparation; Recombinant; SPR Assay; Software; Staining; Standard Deviation; Transfection; Variant Assay
bioz_score: 94
title: A Novel Bacterium-Like Particle Vaccine Displaying the MERS-CoV Receptor-Binding Domain Induces Specific Mucosal and Systemic Immune Responses in Mice
authors: Entao Li; Hang Chi; ...; Xianzhu Xia
journal: Viruses
date: 2019-08-29
pmid: 31470645
doi: 10.3390/v11090799
pmcid: 6784119
cited_by_count: 40
techniques: Cell Culture; Expressing; Fluorescence; Immunofluorescence; Incubation; Infection; Marker; Microscopy; Molecular Weight; Recombinant; SDS Page; Sedimentation; Western Blot
bioz_score: 93
title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning
authors: Yoonji Kim; Hansaem Lee; ...; Dae Young Kim
journal: Antibodies
date: 2019-07-31
pmid: 31544848
doi: 10.3390/antib8030042
pmcid: 6783954
cited_by_count: 19
techniques: Binding Assay; Cell Culture; Clone Assay; Enzyme-linked Immunosorbent Assay; Immunofluorescence; Incubation; Infection; Microscopy; Positive Control; SPR Assay; Sandwich ELISA; Size-exclusion Chromatography
bioz_score: 93
title: Single intranasal immunization with chimpanzee adenovirus-based vaccine induces sustained and protective immunity against MERS-CoV infection
authors: Wenxu Jia; Rudragouda Channappanavar; ...; Linqi Zhang
journal: Emerging microbes & infections
date: 2019-01-01
pmid: 31130102
doi: 10.1080/22221751.2019.1620083
pmcid: 6542157
cited_by_count: 44
techniques: Activity Assay; Binding Assay; Expressing; Infection; Negative Control; Neutralization; Recombinant; Sequencing; Western Blot
bioz_score: 91
title: A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques
authors: Adam S. Cockrell; Joshua C. Johnson; ...; Reed F. Johnson
journal: Scientific Reports
date: 2018-07-16
pmid: 30013082
doi: 10.1038/s41598-018-28900-1
pmcid: 6048037
cited_by_count: 18
techniques: Double Staining; Formalin-fixed Paraffin-Embedded; Immunohistochemistry; Immunostaining
bioz_score: 93
title: MERS-CoV pathogenesis and antiviral efficacy of licensed drugs in human monocyte-derived antigen-presenting cells
authors: Yu Cong; Brit J. Hart; ...; Stefan Pöhlmann
journal: PLoS ONE
date: 2018-03-22
pmid: 29566060
doi: 10.1371/journal.pone.0194868
pmcid: 5864050
cited_by_count: 85
techniques: Activity Assay; Cell Based Assay; Cell Viability Assay; Derivative Assay; Fluorescence; Immunofluorescence; Infection; Inhibition; Staining
bioz_score: 93
title: Identification of the Fusion Peptide-Containing Region in Betacoronavirus Spike Glycoproteins
authors: Xiuyuan Ou; Wangliang Zheng; ...; Zhaohui Qian
journal: Journal of virology
date: 2016-06-15
doi: 10.1128/jvi.00015-16
cited_by_count: 62
techniques: Binding Assay; Flow Cytometry; Ion Exchange Chromatography; Mutagenesis; Plasmid Preparation; Purification; Staining; Transfection
bioz_score: 93
title: Protective Efficacy of Recombinant Modified Vaccinia Virus Ankara Delivering Middle East Respiratory Syndrome Coronavirus Spike Glycoprotein
authors: Asisa Volz; Alexandra Kupke; ...; Gerd Sutter
journal: Journal of virology
date: 2015-05-27
doi: 10.1128/jvi.00614-15
cited_by_count: 131
techniques: Immunohistochemical staining
bioz_score: 93
