| Catalog No. | Size | Price (USD) |
| 40150-D002-20 | 20 µL | $118.49 |
| 40150-D002-50 | 50 µL | $222.17 |
| 40150-D002-100 | 100 µL | $370.28 |
General Information
| Product name | SARS-CoV/SARS-CoV-2 Spike antibody,Chimeric MAb |
| Validated applications | ELISA (FAQ Protocol), FCM (FAQ Protocol), Neutralization |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike RBD Protein (Can block the binding between RBD and ACE2) Has cross-reactivity in ELISA with SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H10) SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 Protein (Cat# 40589-V08B16) SARS-CoV-2 Delta (B.1.617.2) Spike S1 Protein (Cat# 40591-V08H23) SARS-CoV-2 Delta (B.1.617.2) Spike RBD Protein (Cat# 40592-V08H90) SARS-CoV-2 Spike S1 Protein (Cat# 40591-V08H) SARS-CoV-2 Spike RBD Protein (Cat# 40592-V08B) SARS-CoV Spike S1 Protein (Cat# 40150-V08B1) No cross-reactivity in ELISA with SARS-CoV-2 Omicron (XBB.1.16) Spike S1+S2 trimer Protein (Cat# 40589-V08H48) SARS-CoV-2 Omicron (XBB.1.5) Spike S1+S2 trimer Protein (Cat# 40589-V08H45) SARS-CoV-2 Omicron (XBB.1.5) Spike RBD Protein (Cat# 40592-V08H146) SARS-CoV-2 Omicron (XBB.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H42) SARS-CoV-2 Omicron (XBB) Spike RBD Protein (Cat# 40592-V08H144) SARS-CoV-2 Omicron (BQ.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H41) SARS-CoV-2 Omicron (BQ.1.1) Spike RBD Protein (Cat# 40592-V08H143) SARS-CoV-2 Omicron (BF.7) Spike S1+S2 trimer Protein (Cat# 40589-V08H39) SARS-CoV-2 Omicron (BA.4.6/BF.7) Spike RBD Protein (Cat# 40592-V08H140) SARS-CoV-2 Omicron (BA.4.6) Spike S1+S2 trimer Protein (Cat# 40589-V08H37) SARS-CoV-2 Omicron (BA.2.75) Spike RBD Protein (Cat# 40592-V08H135) SARS-CoV-2 Omicron (BA.2.75) Spike S1+S2 trimer Protein (Cat# 40589-V08H36) SARS-CoV-2 Omicron (BA.2.75.2) Spike RBD Protein (Cat# 40592-V08H141) SARS-CoV-2 Omicron (BA.2.75.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H38) SARS-CoV-2 Omicron (BA.2.3.20) Spike RBD Protein (Cat# 40592-V08H142) SARS-CoV-2 Omicron (BA.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H29) SARS-CoV-2 Omicron (BA.1.1) Spike RBD Protein (Cat# 40592-V08H129) SARS-CoV-2 Omicron (B.1.1.529) S1+S2 trimer Protein (Cat# 40589-V08H26) SARS-CoV-2 Omicron (B.1.1.529) Spike S1 Protein (Cat# 40591-V08H41) SARS-CoV-2 Omicron (B.1.1.529) Spike RBD Protein (Cat# 40592-V08H121) SARS-CoV-2 Omicron (BA.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H28) SARS-CoV-2 Omicron (BA.2) Spike S1 Protein (Cat# 40591-V08H43) SARS-CoV-2 Omicron (BA.2) Spike RBD Protein (Cat# 40592-V08H123) SARS-CoV-2 Omicron (BA.2) Spike S1 NTD Protein (Cat# 40591-V08H44) SARS-CoV-2 Omicron (BA.2.12.1) Spike RBD Protein (Cat# 40592-V08H132) SARS-CoV-2 Omicron (BA.4/BA.5/BA.5.2) Spike RBD Protein (Cat# 40592-V08H130) SARS-CoV-2 Omicron (BA.5) Spike RBD Protein (Cat# 40592-V08H131) SARS-CoV-2 XD(BA.1 x AY.4) Spike S1+S2 trimer Protein (Cat# 40589-V08H30) SARS-CoV-2 Delta (B.1.617.2) Spike S1 NTD Protein (Cat# 40591-V08H36) MERS-CoV Spike S1 Protein (Cat# 40069-V08B1) HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H) HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H) HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H) HCoV-229E Spike S1 Protein (Cat# 40601-V08H) HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B) |
| Immunogen | Recombinant SARS-CoV Spike RBD Protein (Catalog#40150-V08B2) |
| Preparation | It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology. |
| Source | Monoclonal mouse (variable region) / human (kappa / IgG1 constant) chimeric antibody Clone #D002 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40150-D002) was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC 50 (WT) is typically 3.9 nM. |
| Image 1 Alt Text | SARS Coronavirus spike Enzyme linked immunosorbent assay (ELISA) 31933 |
| Image 1 URL | Source image |
| Image 2 Description | Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40150-D002) was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC 50 (WT) is typically 3.9 nM. |
| Image 2 Alt Text | SARS Coronavirus spike Microneutralizaiton (MN) 31934 |
| Image 2 URL | Source image |
| Image 3 Description | Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40150-D002) was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC 50 (WT) is typically 3.9 nM. |
| Image 3 Alt Text | SARS Coronavirus spike Flow Cytometry (FC) 32888 |
| Image 3 URL | Source image |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
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journal: Advanced Therapeutics
date: 2025-10-20
doi: 10.1002/adtp.202500244
cited_by_count: 0
techniques: Control; Negative Control; Positive Control
bioz_score: 93
title: Multivalent Protein Nanorings for Broad and Potent SARS-CoV-2 Neutralization.
authors: Molood Behbahanipour; Carla Goldin; ...; Salvador Ventura
journal: Advanced Healthcare Materials
date: 2025-10-05
pmid: 41047514
doi: 10.1002/adhm.202503487
cited_by_count: 1
techniques: Control; Incubation; Inhibition
bioz_score: 93
title: Coronavirus vaccine
authors: Susanne Rauch; Hans Wolfgang Große; Benjamin Petsch
date: 2025-01-14
cited_by_count: 0
techniques: Activity Assay; Concentration Assay; Control; Enzyme-linked Immunosorbent Assay; Incubation; Software; Virus
bioz_score: 93
title: Tip Optofluidic Immunoassay: Evaluating COVID-19 Antibody Protection with 1μL Fingertip Blood
authors: Xiaotian Tan; Yujuan Chai; ...; Hui Yang
journal: Research Square
date: 2024-10-17
doi: 10.21203/rs.3.rs-5174434/v1
cited_by_count: 0
techniques: Binding Assay; Injection
bioz_score: 93
title: Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown
authors: Supriya Atta; Yuanhao Zhao; ...; Tuan Vo-Dinh
journal: Analytical Chemistry
date: 2024-03-12
pmid: pm38471066
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techniques: Microscopy; Saline; Transmission Assay
bioz_score: 92
title: Naked-Eye Readable Microarray for Rapid Profiling of Antibodies against Multiple SARS-CoV-2 Variants
authors: Jingjie Nan; Yuan Chen; ...; Bai Yang
journal: Nano Letters
date: 2023-12-04
pmid: pm38047611
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bioz_score: 92
title: Printed Capacitive Immunoassay for Detecting SARS-CoV-2 Viral Particles
authors: Muhammadeziz Tursunniyaz; Joseph Andrews
journal: IEEE Sensors Journal
date: 2023-10-15
doi: 10.1109/jsen.2022.3198598
cited_by_count: 2
techniques: Binding Assay; Saline
bioz_score: 92
title: Introduction of Nanogap-Enhanced SERS Nanotags into a Lateral Flow Assay for Ultrasensitive and Simultaneous Monitoring of SARS-CoV‑2 S and NP Antigens
authors: Xiaoxian Liu; Xingsheng Yang; ...; Rui Xiao
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date: 2023-05-11
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cited_by_count: 0
techniques: Recombinant
bioz_score: 92
title: Lectin Fingerprinting Distinguishes Antibody Neutralization in SARS-CoV-2
authors: Michael G. Wuo; Amanda E. Dugan; ...; Laura L. Kiessling
journal: ACS Central Science
date: 2023-05-10
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pmcid: 10214521
cited_by_count: 0
techniques: Recombinant
bioz_score: 92
title: Evidence for Aerosol Transfer of SARS-CoV-2–Specific Humoral Immunity
authors: Ross M. Kedl; Elena W. Y. Hsieh; ...; Rosemary Rochford
journal: ImmunoHorizons
date: 2023-05-01
pmid: 37159005
doi: 10.4049/immunohorizons.2300027
pmcid: 10579981
cited_by_count: 2
techniques: Aerosol; Binding Assay; Conjugation Assay; Incubation; Multiplex Assay; Negative Control; Positive Control; Staining; Transformation Assay
bioz_score: 92
title: Improving mRNA vaccine safety and efficiency with cationized lipid nanoparticle formula
authors: Peng Xu; Liao Guangneng; ...; Xu Kai
journal: bioRxiv
date: 2023-03-30
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cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay; Incubation
bioz_score: 92
title: COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray
authors: Jacob T. Heggestad; Rhett J. Britton; ...; Ashutosh Chilkoti
journal: Analytical Chemistry
date: 2023-03-24
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techniques: Comparison
bioz_score: 92
title: OligoBinders: Bioengineered Soluble Amyloid-like Nanoparticles to Bind and Neutralize SARS-CoV-2
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journal: ACS Applied Materials & Interfaces
date: 2023-02-22
pmid: 36890692
doi: 10.1021/acsami.2c18305
pmcid: 9969896
cited_by_count: 2
techniques: Incubation; Luciferase
bioz_score: 93
title: Omicron variants escape the persistent SARS-CoV-2-specific antibody response in 2-year COVID-19 convalescents regardless of vaccination.
authors: Miao Wang; Bing Zhou; ...; Zheng Zhang
journal: Emerging microbes & infections
date: 2022-12-29
pmid: 36444724
doi: 10.1080/22221751.2022.2151381
cited_by_count: 11
techniques: Binding Assay; Chemiluminescence Immunoassay; Clinical Proteomics; Flow Cytometry; Software; Staining
bioz_score: 93
title: SARS-CoV-2 Omicron variant is attenuated for replication in a polarized human lung epithelial cell model.
authors: Christin Mache; Jessica Schulze; ...; Thorsten Wolff
journal: Communications Biology
date: 2022-10-31
pmid: 36302956
cited_by_count: 43
techniques: Electron Microscopy; Fluorescence; Infection; Microscopy; Staining
bioz_score: 93
title: Development of a rapid, simple, and sensitive point-of-care technology platform utilizing ternary NanoLuc
authors: Emily A. Torio; Valerie T. Ressler; ...; Melanie L. Dart
journal: Frontiers in Microbiology
date: 2022-10-26
pmid: 36386626
pmcid: 9643700
cited_by_count: 2
techniques: Antibody Labeling; Antigen Assay; Binding Assay; Bioprocessing; Concentration Assay; Incubation; Labeling; Purification
bioz_score: 92
title: SARS-CoV-2 Infection in Unvaccinated High Risk Pregnant Women in the Bronx, NY is Associated with Placental Abnormalities, Shorter Gestation and Lower Apgar Scores
authors: Sandra Reznik; Patricia Vuguin; ...; Maureen Charron
journal: Europe PMC
date: 2022-09-05
doi: 10.20944/preprints202209.0063.v1
cited_by_count: 0
techniques: Control; Formalin-fixed Paraffin-Embedded; In Situ Hybridization; Infection; Polymerase Chain Reaction; Positive Control; Staining
bioz_score: 93
title: Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
authors: Shouzhi Yu; Yangyang Wei; ...; Yinzhong Shen
journal: Viruses
date: 2022-08-31
pmid: 36146745
doi: 10.3390/v14091938
pmcid: 9503440
cited_by_count: 11
techniques: Binding Assay; Concentration Assay; Injection; SPR Assay; Software
bioz_score: 92
title: An immunological autobiography: my year as a COVID-19 vaccine trial participant
authors: Ross M. Kedl
journal: NPJ vaccines
date: 2022-07-18
pmid: 35851389
doi: 10.1038/s41541-022-00502-z
pmcid: 9293989
cited_by_count: 1
techniques: Conjugation Assay; Derivative Assay; Flow Cytometry; Fluorescence; Incubation; Positive Control; Staining
bioz_score: 93
title: Vaccine, Booster and Natural Antibody Binding to SARS-CoV-2 Omicron (BA.1) Spike Protein and Vaccine Efficacy
authors: James-Pemberton Philip H.; Helliwell Mark W.; ...; Shaw Andrew M.
journal: medRxiv
date: 2022-07-15
doi: 10.1101/2022.07.12.22277539
cited_by_count: 0
techniques: Recombinant
bioz_score: 93
title: Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development
authors: Nol Salcedo; Ankita Reddy; ...; Paul J. Brindley
journal: PLoS Neglected Tropical Diseases
date: 2022-03-31
pmid: 35358198
doi: 10.1371/journal.pntd.0010311
pmcid: 9004783
cited_by_count: 9
techniques: Flow Cytometry; Fluorescence; Immunostaining; Incubation; Positive Control; Western Blot
bioz_score: 92
title: Screening of Botanical Drugs against SARS-CoV-2 Entry Reveals Novel Therapeutic Agents to Treat COVID-19
authors: Junyuan Cao; Yang Liu; ...; Craig E. Cameron
journal: Viruses
date: 2022-02-08
pmid: 35215943
doi: 10.3390/v14020353
pmcid: 8877376
cited_by_count: 14
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Positive Control
bioz_score: 92
title: Rapid field determination of SARS-CoV-2 by a colorimetric and fluorescent dual-functional lateral flow immunoassay biosensor
authors: Han Han; Chongwen Wang; ...; Rui Xiao
journal: Sensors and Actuators B: Chemical
date: 2021-10-09
pmid: 34658530
doi: 10.1016/j.snb.2021.130897
pmcid: 8500848
cited_by_count: 92
techniques: Enzyme-linked Immunosorbent Assay
bioz_score: 92
title: Quantitative epitope analysis reveals drastic 63% reduced immuno-affinity and 60% enhanced transmissibility for SARS-CoV-2 variants
authors: Giulio Brunetti; Annalisa De Pastina; Martin Hegner
journal: Nanoscale Advances
date: 2021-09-23
pmid: 36132357
doi: 10.1039/d1na00554e
pmcid: 9419875
cited_by_count: 2
techniques: Binding Assay; In Situ; Injection; Modification; Mutagenesis; Neutralization; Recombinant; Standard Deviation; Variant Assay
bioz_score: 92
title: A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels
authors: Juliano Alves; Laurie Engel; ...; Hicham Zegzouti
journal: Scientific Reports
date: 2021-09-16
pmid: 34531417
doi: 10.1038/s41598-021-97330-3
pmcid: 8445915
cited_by_count: 21
techniques: Activity Assay; Binding Assay; Competitive Binding Assay; Derivative Assay; Enzyme-linked Immunosorbent Assay; Generated; In Vitro; Incubation; Inhibition; Luciferase; Neutralization; Plaque Reduction Neutralization Test; Serologic Assay; Titration; Two Tailed Test
bioz_score: 92
title: Ultrasensitive and Simultaneous Detection of Two Specific SARS-CoV-2 Antigens in Human Specimens Using Direct/Enrichment Dual-Mode Fluorescence Lateral Flow Immunoassay
authors: Chongwen Wang; Xiaodan Cheng; ...; Shengqi Wang
journal: ACS Applied Materials & Interfaces
date: 2021-08-20
pmid: 34412466
doi: 10.1021/acsami.1c11461
pmcid: 8386037
cited_by_count: 45
techniques: Electron Microscopy; Enzyme-linked Immunosorbent Assay; Microscopy; Pore Size; Purification; Transmission Assay
bioz_score: 92
title: A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays
authors: Yan Ni; Bas J. H. M. Rosier; ...; Maarten Merkx
journal: Nature Communications
date: 2021-07-28
pmid: 34321486
doi: 10.1038/s41467-021-24874-3
pmcid: 8319308
cited_by_count: 51
bioz_score: 92
title: Screening Antibodies Raised against the Spike Glycoprotein of SARS-CoV-2 to Support the Development of Rapid Antigen Assays
authors: Jason L. Cantera; David M. Cate; ...; David S. Boyle
journal: ACS Omega
date: 2021-07-27
pmid: 34373846
doi: 10.1021/acsomega.1c01321
pmcid: 8340086
cited_by_count: 8
bioz_score: 93
title: A human-airway-on-a-chip for the rapid identification of candidate antiviral therapeutics and prophylactics
authors: Longlong Si; Haiqing Bai; ...; Donald E. Ingber
journal: Nature Biomedical Engineering
date: 2021-05-12
pmid: 33941899
doi: 10.1038/s41551-021-00718-9
pmcid: 8387338
cited_by_count: 222
techniques: Binding Assay; Control; Expressing; Plasmid Preparation; Recombinant; Transfection; Virus; Western Blot
bioz_score: 93
title: A high-throughput microfluidic nanoimmunoassay for detecting anti–SARS-CoV-2 antibodies in serum or ultralow-volume blood samples
authors: Zoe Swank; Grégoire Michielin; ...; Sebastian J. Maerkl
journal: Proceedings of the National Academy of Sciences of the United States of America
date: 2021-05-04
pmid: 33945500
doi: 10.1073/pnas.2025289118
pmcid: 8106336
cited_by_count: 44
bioz_score: 93
title: A high-throughput microfluidic nano-immunoassay for detecting anti-SARS-CoV-2 antibodies in serum or ultra-low volume dried blood samples
authors: Swank Zoe; Michielin Grégoire; ...; Maerkl Sebastian J.
journal: medRxiv
date: 2021-02-08
doi: 10.1101/2020.10.07.20208280
cited_by_count: 0
techniques: Construct; Labeling; Transfection
bioz_score: 92
title: A 10-Minute “Mix and Read” Antibody Assay for SARS-CoV-2
authors: Juuso Rusanen; Lauri Kareinen; ...; Helle Bielefeldt-Ohmann
journal: Viruses
date: 2021-01-20
pmid: 33498157
doi: 10.3390/v13020143
pmcid: 7908974
cited_by_count: 26
techniques: Cell Culture; Enzyme-linked Immunosorbent Assay; Expressing; Förster Resonance Energy Transfer; Imaging; Incubation; Labeling; Modification; Purification; Selection; Transfection
bioz_score: 93
title: Human organ chip-enabled pipeline to rapidly repurpose therapeutics during viral pandemics
authors: Si Longlong; Bai Haiqing; ...; Ingber Donald E.
journal: bioRxiv
date: 2020-08-19
doi: 10.1101/2020.04.13.039917
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Cell Culture; Expressing; Immunofluorescence; Incubation; Infection; Inhibition; Luciferase; Plasmid Preparation; Positive Control; Quantitative RT-PCR; Recombinant; Staining; Transfection; Western Blot
bioz_score: 93
