| Catalog No. | Size | Price (USD) |
| 40150-D004-H-20 | 20 µg | $177.73 |
| 40150-D004-H-100 | 100 µg | $555.42 |
General Information
| Product name | SARS-CoV / SARS-CoV-2 Spike Antibody (HRP), Chimeric MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV Spike RBD |
| Immunogen | Recombinant SARS-CoV Spike RBD Protein (Catalog#40150-V08B2) |
| Preparation | It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology and conjugated with horseradish-peroxidase (HRP). |
| Source | Monoclonal mouse (variable region) / human (kappa / IgG1 constant) chimeric antibody Clone #D004 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | HRP |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: Mannosylated polyethylenimine-cholesterol-based nanoparticles for targeted delivery of minicircle DNA vaccine against COVID-19 to antigen-presenting cells.
authors: Dalinda Eusébio; Milan Paul; ...; Ângela Sousa
journal: International Journal of Pharmaceutics
date: 2024-04-09
pmid: 38430949
doi: 10.1016/j.ijpharm.2024.123959
cited_by_count: 5
techniques: Membrane; Western Blot
bioz_score: 92
