| Catalog No. | Size | Price (USD) |
| 40150-MM02-20 | 20 µL | $118.49 |
| 40150-MM02-50 | 50 µL | $222.17 |
| 40150-MM02-100 | 100 µL | $370.28 |
General Information
| Product name | SARS-CoV Spike S1 Subunit Antibody, Mouse MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV Spike S1 No cross-reactivity in ELISA with SARS-CoV-2 Spike S1 Protein (Cat# 40591-V08B1) MERS-CoV Spike S1 Protein (Cat# 40069-V08B1) HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H) HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H) HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H) HCoV-229E Spike S1 Protein (Cat# 40601-V08H) HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B) |
| Immunogen | Recombinant SARS-CoV Spike S1 Subunit Protein (Catalog#40150-V08B1) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Spike S1 Subunit (Catalog#40150-V08B1; AAX16192.1; Met1-Arg667). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #02 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
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techniques: Cell Culture; Immunoprecipitation; Incubation
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bioz_score: 94
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techniques: Activity Assay; Expressing; Luciferase; Microscopy; Mutagenesis; Plasmid Preparation; Reporter Assay; SDS Page; Western Blot
bioz_score: 94
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techniques: Binding Assay; Construct; Cotransfection; Derivative Assay; Expressing; Flow Cytometry; Fluorescence; Infection; Lysis; Mutagenesis; Protease Inhibitor; Software; Staining; Transfection; Two Tailed Test; Variant Assay; Western Blot
bioz_score: 94
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cited_by_count: 50
techniques: Construct; Expressing; Imaging; Radio IP Assay; Recombinant; Transfection; Western Blot
bioz_score: 94
title: Neutralization and Stability of SARS-CoV-2 Omicron Variant
authors: Zeng Cong; Evans John P.; ...; Liu Shan-Lu
journal: bioRxiv
date: 2021-12-20
doi: 10.1101/2021.12.16.472934
cited_by_count: 0
techniques: Cell Culture; Construct; Incubation; Mutagenesis; SDS Page; Transfection
bioz_score: 94
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journal: Journal of virology
date: 2021-11-24
pmid: 34817202
doi: 10.1128/jvi.01626-21
pmcid: 8827021
cited_by_count: 23
techniques: Activity Assay; Binding Assay; Cell Culture; Centrifugation; Cryo-EM Sample Prep; Expressing; Fluorescence; Functional Assay; Incubation; Infection; Luciferase; Microscopy; Mutagenesis; Purification; SDS Page; Silver Staining; Staining; Transfection; Variant Assay; Western Blot
bioz_score: 93
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techniques: Cell Based Assay; Expressing; Fluorescence; Infection; Stable Transfection; Transfection
bioz_score: 94
title: COVID- e Vax, an electroporated plasmid DNA vaccine candidate encoding the SARS-CoV-2 Receptor Binding Domain, elicits protective immune responses in animal models of COVID-19
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journal: bioRxiv
date: 2021-09-01
doi: 10.1101/2021.06.14.448343
cited_by_count: 0
techniques: Binding Assay; Construct; Electroporation; Enzyme-linked Immunosorbent Assay; Enzyme-linked Immunospot; Expressing; Imaging; Injection; Negative Control; Neutralization; Plasmid Preparation; Recombinant; Sequencing; Staining; Transfection; Western Blot
bioz_score: 94
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date: 2021-05-11
pmid: 33979600
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techniques: Confocal Microscopy; Immunofluorescence; Incubation; Microscopy
bioz_score: 93
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journal: Cell death and differentiation
date: 2021-04-20
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pmcid: 8056997
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techniques: Binding Assay; Cell Culture; Control; Expressing; Immunostaining; Incubation; Infection; Labeling; Membrane; Multiplex Assay; Mutagenesis; Residue; Sequencing; Staining; Virus; Western Blot
bioz_score: 93
title: The Rhinolophus affinis bat ACE2 and multiple animal orthologs are functional receptors for bat coronavirus RaTG13 and SARS-CoV-2
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journal: Science Bulletin
date: 2021-01-19
pmid: 33495713
doi: 10.1016/j.scib.2021.01.011
pmcid: 7816560
cited_by_count: 40
techniques: Binding Assay; Expressing; Flow Cytometry; Fluorescence; Incubation; Luciferase; Plasmid Preparation; Sequencing; Stable Transfection; Transduction; Transfection; Western Blot
bioz_score: 93
title: Antibody neutralization of SARS-CoV-2 through ACE2 receptor mimicry
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journal: Nature Communications
date: 2021-01-11
pmid: 33431856
doi: 10.1038/s41467-020-20501-9
pmcid: 7801515
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techniques: Affinity Purification; Biomarker Discovery; Concentration Assay; Produced; Virus
bioz_score: 93
title: Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva
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journal: Biosensors & bioelectronics
date: 2020-10-03
pmid: 33086175
doi: 10.1016/j.bios.2020.112686
pmcid: 7833515
cited_by_count: 395
techniques: Amplification; Binding Assay; Blocking Assay; CRISPR; Cell Culture; Concentration Assay; Enzyme-linked Immunosorbent Assay; Fluorescence; Immunochromatographic Assay; Incubation; Modification; Negative Control; Produced; Recombinant; Reporter Assay; SPR Assay; Standard Deviation; Transduction
bioz_score: 93
title: A Targeted Vaccine against COVID-19: S1-Fc Vaccine Targeting the Antigen-Presenting Cell Compartment Elicits Protection against SARS-CoV-2 Infection
authors: Herrmann Andreas; Maruyama Junki; ...; Ji Henry
journal: bioRxiv
date: 2020-06-30
doi: 10.1101/2020.06.29.178616
cited_by_count: 0
techniques: Blocking Assay; Incubation; Serial Dilution
bioz_score: 93
title: Patients with immune-mediated inflammatory diseases receiving cytokine inhibitors have low prevalence of SARS-CoV-2 infection
authors: David Simon; Koray Tascilar; ...; Georg Schett
journal: Europe PMC
date: 2020-06-10
doi: 10.21203/rs.3.rs-34550
cited_by_count: 0
techniques: Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Infection; Recombinant
bioz_score: 94
title: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV
authors: Xiuyuan Ou; Yan Liu; ...; Zhaohui Qian
journal: Nature Communications
date: 2020-03-27
pmid: 32221306
doi: 10.1038/s41467-020-15562-9
pmcid: 7100515
cited_by_count: 2709
techniques: Binding Assay; Expressing; Flow Cytometry; Incubation; Mutagenesis; Neutralization; Plasmid Preparation; Protease Inhibitor; Sequencing; Transduction; Transfection; Western Blot
bioz_score: 93
title: Characterization of spike glycoprotein of 2019-nCoV on virus entry and its immune cross-reactivity with spike glycoprotein of SARS-CoV
authors: Xiuyuan Ou; Yan Liu; ...; Zhaohui Qian
journal: Europe PMC
date: 2020-02-18
doi: 10.21203/rs.2.24016
cited_by_count: 0
techniques: Protease Inhibitor
bioz_score: 93
title: Identification of the Fusion Peptide-Containing Region in Betacoronavirus Spike Glycoproteins
authors: Xiuyuan Ou; Wangliang Zheng; ...; Zhaohui Qian
journal: Journal of virology
date: 2016-06-15
doi: 10.1128/jvi.00015-16
cited_by_count: 62
techniques: Binding Assay; Flow Cytometry; Ion Exchange Chromatography; Mutagenesis; Plasmid Preparation; Purification; Staining; Transfection
bioz_score: 94
