| Catalog No. | Size | Price (USD) |
| 40150-R327-20 | 20 µL | $118.49 |
| 40150-R327-100 | 100 µL | $370.28 |
General Information
| Product name | SARS-COV Spike Antibody, Rabbit MAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV Spike |
| Immunogen | Recombinant SARS-CoV Spike Protein (Catalog#40150-V08B2) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant SARS-CoV Spike (Catalog#40150-V08B2; AAX16192.1; Arg306-Phe527). |
| Source | Monoclonal Rabbit IgG Clone #327 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-SARS-CoV Spike rabbit monoclonal antibody at 1:1000 dilution. Sample: Recombinant Protein 10 ng Lane A: SARS-CoV Spike/RBD Protein (RBD, His Tag) (Catalog# 40150-V08B2) Lane B: SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Catalog# 40592-V05H) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | SARS Coronavirus spike Western blot (WB) 36297 |
| Image 1 URL | Source image |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: Identification of dominant peptide epitopes and antibody response to the spike protein of SARS-CoV-2
authors: Xianyan Zhang; Peipei Xu; ...; Xuemin Guo
journal: Frontiers in cellular and infection microbiology
date: 2025-10-15
pmid: 41170287
cited_by_count: 0
techniques: SDS Page; Sequencing; Silver Staining; Simple Western; Software; Staining; Western Blot
bioz_score: 93
title: The Long-Term Immunity of a Microneedle Array Patch of a SARS-CoV-2 S1 Protein Subunit Vaccine Irradiated by Gamma Rays in Mice
authors: Eun Kim; Muhammad S. Khan; ...; Apostolos P. Georgopoulos
journal: Vaccines
date: 2025-01-18
pmid: 39852865
pmcid: 11768753
cited_by_count: 1
techniques: Affinity Purification; Binding Assay; Blocking Assay; Comparison; Construct; Control; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Incubation; Inhibition; Irradiation; Isolation; MANN-WHITNEY; Marker; Microneutralization Assay; Molecular Weight; Neutralization; Plasmid Preparation; Purification; Recombinant; SDS Page; Sampling; Silver Staining; Staining; Standard Deviation; Titration; Transfection; Vaccines; Variant Assay; Virus; Western Blot; Whisker Assay
bioz_score: 93
title: Long-term Immunity of a Microneedle Array Patch of SARS-CoV-2 S1 Protein Subunit Vaccine Irradiated by Gamma Rays in Mice
authors: Kim Eun; Khan Muhammad S.; ...; Gambotto Andrea
journal: bioRxiv
date: 2024-10-25
cited_by_count: 0
techniques: Affinity Purification; Binding Assay; Blocking Assay; Comparison; Construct; Control; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Incubation; Inhibition; Irradiation; Isolation; MANN-WHITNEY; Marker; Microneutralization Assay; Molecular Weight; Neutralization; Plasmid Preparation; Purification; Recombinant; SDS Page; Sampling; Silver Staining; Staining; Standard Deviation; Titration; Transfection; Vaccines; Variant Assay; Virus; Western Blot; Whisker Assay
bioz_score: 93
title: Engineered migrasomes provide a robust and thermally stable vaccination platform
journal: eLife
date: 2024-05-20
doi: 10.7554/elife.97621
cited_by_count: 0
techniques: Derivative Assay; Enzyme-linked Immunosorbent Assay; Imaging; Plasmid Preparation; Recombinant; Western Blot
bioz_score: 93
title: Dauricine interferes with SARS-CoV-2 variants infection by blocking the interface between RBD and ACE2.
authors: Lei Xie; Sai Shi; ...; Sheng Ye
journal: International Journal of Biological Macromolecules
date: 2023-11-27
pmid: 37848107
doi: 10.1016/j.ijbiomac.2023.127344
cited_by_count: 5
techniques: Activity Assay; Enzyme-linked Immunospot; Neutralization; Staining
bioz_score: 93
title: Antibodies targeting a quaternary site on SARS-CoV-2 spike glycoprotein prevent viral receptor engagement by conformational locking
authors: Lihong Liu; Ryan G. Casner; ...; David D. Ho
journal: Immunity
date: 2023-10-10
pmid: 37776849
doi: 10.1016/j.immuni.2023.09.003
pmcid: 10588992
cited_by_count: 32
techniques: Electron Microscopy; Generated; Incubation; Inhibition; Luciferase; Mutagenesis; Recombinant; Software; Staining; Variant Assay; Virus; Western Blot
bioz_score: 93
title: SARS-CoV-2 Omicron entry is type II transmembrane serine protease-mediated in human airway and intestinal organoid models
authors: Anna Z. Mykytyn; Tim I. Breugem; ...; Mart M. Lamers
journal: Journal of virology
date: 2023-08-09
pmid: 37555660
pmcid: 10506477
cited_by_count: 41
techniques: Nucleic Acid Electrophoresis; Single Vesicle Fusion Assay; Software; Staining; Virus; Western Blot
bioz_score: 93
title: Neuropathology in COVID-19 autopsies is defined by microglial activation and lesions of the white matter with emphasis in cerebellar and brain stem areas
authors: Julian A. Stein; Manuel Kaes; ...; Walter J. Schulz-Schaeffer
journal: Frontiers in Neurology
date: 2023-07-13
pmcid: 10374362
cited_by_count: 3
bioz_score: 93
title: Longitudinal analyses using 18 F-Fluorodeoxyglucose positron emission tomography with computed tomography as a measure of COVID-19 severity in the aged, young, and humanized ACE2 SARS-CoV-2 hamster models.
authors: Yu Cong; Ji Hyun Lee; ...; Reed F Johnson
journal: Antiviral research
date: 2023-05-15
pmid: 37068595
cited_by_count: 3
techniques: Blocking Assay; Fluorescence; High Content Screening; Incubation; Neutralization; Software; Staining
bioz_score: 94
title: Tetravalent SARS-CoV-2 S1 Subunit Protein Vaccination Elicits Robust Humoral and Cellular Immune Responses in SIV-Infected Rhesus Macaque Controllers
authors: Khan Muhammad S.; Kim Eun; ...; Apetrei Cristian
journal: bioRxiv
date: 2023-03-16
doi: 10.1101/2023.03.15.532808
cited_by_count: 0
techniques: Affinity Purification; Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Flow Cytometry; Incubation; Isolation; Marker; Microneutralization Assay; Molecular Weight; Mutagenesis; Negative Control; Plasmid Preparation; Purification; SDS Page; Sampling; Silver Staining; Staining; Titration; Transfection; Western Blot
bioz_score: 94
title: Trivalent SARS-CoV-2 S1 Subunit Protein Vaccination Induces Broad Humoral Responses in BALB/c Mice
authors: Muhammad S. Khan; Eun Kim; ...; Michal Herman-Edelstein
journal: Vaccines
date: 2023-01-31
pmcid: 9967783
cited_by_count: 0
techniques: Binding Assay; Blocking Assay; Derivative Assay; Enzyme-linked Immunosorbent Assay; Expressing; Incubation; Inhibition; Mutagenesis; Plasmid Preparation; Purification; Recombinant; SDS Page; Silver Staining; Staining; Variant Assay; Western Blot; Whisker Assay
bioz_score: 93
title: Immunogenicity of COVID-eVax Is Moderately Impacted by Temperature and Molecular Isoforms
authors: D’Alessio Federico; Lione Lucia; ...; Palombo Fabio
journal: bioRxiv
date: 2022-12-29
doi: 10.1101/2022.12.29.522202
cited_by_count: 0
techniques: Affinity Purification; Concentration Assay; Enzyme-linked Immunosorbent Assay; Incubation; Potency Assay; Transfection
bioz_score: 93
title: Antiviral innate immunity is diminished in the upper respiratory tract of severe COVID-19 patients
authors: Ramos-Benitez Marcos J.; Strich Jeffrey R.; ...; Chertow Daniel S.
journal: medRxiv
date: 2022-11-13
doi: 10.1101/2022.11.08.22281846
cited_by_count: 0
techniques: Imaging; Incubation; Infection; Staining
bioz_score: 94
title: Raman Fingerprints of the SARS-CoV-2 Delta Variant and Mechanisms of Its Instantaneous Inactivation by Silicon Nitride Bioceramics.
authors: Giuseppe Pezzotti; Eriko Ohgitani; ...; Osam Mazda
journal: ACS Infectious Diseases
date: 2022-08-15
pmid: 35819780
cited_by_count: 7
techniques: Staining
bioz_score: 93
title: Adenovirus-vectored SARS-CoV-2 vaccine expressing S1-N fusion protein
authors: Muhammad S Khan; Eun Kim; ...; Andrea Gambotto
journal: Antibody Therapeutics
date: 2022-07-01
pmid: 35967905
doi: 10.1093/abt/tbac015
pmcid: 9372896
cited_by_count: 8
techniques: Blocking Assay; Enzyme-linked Immunosorbent Assay; Enzyme-linked Immunospot; Flow Cytometry; Homologous Recombination; Incubation; Infection; Injection; Isolation; Negative Control; Neutralization; Plaque Reduction Neutralization Test; Plasmid Preparation; Positive Control; Recombinant; Variant Assay; Western Blot
bioz_score: 93
title: Exposure Route Influences Disease Severity in the COVID-19 Cynomolgus Macaque Model
authors: Sandra L. Bixler; Christopher P. Stefan; ...; F. Javier Salguero
journal: Viruses
date: 2022-05-10
pmid: 35632755
doi: 10.3390/v14051013
pmcid: 9145782
cited_by_count: 14
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Expressing; Immunofluorescence; Incubation; Infection; Plaque Assay; Plasmid Preparation; Quantitative RT-PCR; Software; Staining; Whisker Assay
bioz_score: 93
title: Acute Kidney Injury and Urinary and Histopathological Disorders in Kidney Transplant Patients with SARS-CoV-2 Infection
authors: L.A. Vigara; F. Villanego; ...; A. Mazuecos
journal: Transplantation proceedings
date: 2022-04-11
pmid: 35649967
doi: 10.1016/j.transproceed.2022.04.008
pmcid: 8995419
cited_by_count: 1
techniques: Avidin-Biotin Assay; Immunohistochemical staining; Incubation; Infection; Staining
bioz_score: 93
title: SARS-CoV-2 Omicron efficiently infects human airway, but not alveolar epithelium
authors: Lamers Mart M.; Mykytyn Anna Z.; ...; Haagmans Bart L.
journal: bioRxiv
date: 2022-01-20
doi: 10.1101/2022.01.19.476898
cited_by_count: 0
techniques: Concentration Assay; Infection; Isolation; SDS Page; Single Vesicle Fusion Assay; Software; Staining; Western Blot
bioz_score: 93
title: Analysis of Glycosylation and Disulfide Bonding of Wild-Type SARS-CoV-2 Spike Glycoprotein
authors: Shijian Zhang; Eden P. Go; ...; Tom Gallagher
journal: Journal of virology
date: 2021-11-24
pmid: 34817202
doi: 10.1128/jvi.01626-21
pmcid: 8827021
cited_by_count: 23
techniques: Activity Assay; Binding Assay; Cell Culture; Centrifugation; Cryo-EM Sample Prep; Expressing; Fluorescence; Functional Assay; Incubation; Infection; Luciferase; Microscopy; Mutagenesis; Purification; SDS Page; Silver Staining; Staining; Transfection; Variant Assay; Western Blot
bioz_score: 93
title: Stillbirth and fetal capillary infection by SARS-CoV-2
authors: Cira di Gioia; Fabrizio Zullo; ...; Roberto Brunelli
journal: American journal of obstetrics & gynecology MFM
date: 2021-10-24
pmid: 34700024
doi: 10.1016/j.ajogmf.2021.100523
pmcid: 8541829
cited_by_count: 18
techniques: Immunohistochemical staining; Immunoperoxidase Staining; Incubation; Light Microscopy; Staining
bioz_score: 93
title: A single subcutaneous or intranasal immunization with adenovirus‐based SARS‐CoV‐2 vaccine induces robust humoral and cellular immune responses in mice
authors: Eun Kim; Florian J. Weisel; ...; Andrea Gambotto
journal: European Journal of Immunology
date: 2021-05-06
pmid: 33772778
doi: 10.1002/eji.202149167
pmcid: 8250272
cited_by_count: 30
techniques: Binding Assay; Blocking Assay; Homologous Recombination; Incubation; Infection; Negative Control; Plasmid Preparation; Positive Control; Recombinant; Western Blot
bioz_score: 93
title: Aerosol Exposure of Cynomolgus Macaques to SARS-CoV-2 Results in More Severe Pathology than Existing Models
authors: Bixler Sandra L.; Stefan Christopher P.; ...; Pitt Margaret L. M.
journal: bioRxiv
date: 2021-04-27
doi: 10.1101/2021.04.27.441510
cited_by_count: 0
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Expressing; Immunofluorescence; Incubation; Plasmid Preparation; Software; Staining
bioz_score: 93
title: Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
authors: Mart M Lamers; Anna Z Mykytyn; ...; Jos WM van der Meer
journal: eLife
date: 2021-04-09
pmid: 33835028
doi: 10.7554/eLife.66815
pmcid: 8131099
cited_by_count: 71
techniques: Concentration Assay; Imaging; Immunohistochemistry; Infection; Low Protein Binding; Microscopy; Molecular Weight; Multiplex Assay; Mutagenesis; Plasmid Preparation; Purification; SDS Page; Sequencing; Single Vesicle Fusion Assay; Software; Staining; Western Blot
bioz_score: 93
title: A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate.
authors: Lorena Sanchez-Felipe; Thomas Vercruysse; ...; Kai Dallmeier
journal: Nature
date: 2021-02-16
pmid: 33260195
doi: 10.1038/s41586-020-3035-9
cited_by_count: 142
techniques: Biomarker Discovery; Chromatin Immunoprecipitation; Flow Cytometry; Immunofluorescence; Magnetic Resonance Imaging; Mutagenesis; Transplantation Assay; Western Blot
bioz_score: 93
title: SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site
authors: Anna Z Mykytyn; Tim I Breugem; ...; Miles P Davenport
journal: eLife
date: 2021-01-04
pmid: 33393462
doi: 10.7554/eLife.64508
pmcid: 7806259
cited_by_count: 101
techniques: Binding Assay; Concentration Assay; Expressing; Immunohistochemistry; Infection; Low Protein Binding; Mass Spectrometry; Microscopy; Mutagenesis; Nucleic Acid Electrophoresis; Over Expression; Plasmid Preparation; Recombinant; Silver Staining; Software; Stable Transfection; Staining; Western Blot
bioz_score: 93
title: Disruption of Adaptive Immunity Enhances Disease in SARS-CoV-2-Infected Syrian Hamsters
authors: Rebecca L. Brocato; Lucia M. Principe; ...; Jay W. Hooper
journal: Journal of virology
date: 2020-10-27
doi: 10.1128/jvi.01683-20
cited_by_count: 50
techniques: Incubation; Plasmid Preparation; Software
bioz_score: 94
title: Human angiotensin-converting enzyme 2 transgenic mice infected with SARS-CoV-2 develop severe and fatal respiratory disease.
authors: Joseph W Golden; Curtis R Cline; ...; Jay W Hooper
journal: JCI insight
date: 2020-10-13
pmid: 32841215
cited_by_count: 176
techniques: Incubation; Plasmid Preparation; Software
bioz_score: 94
title: Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva
authors: Laura Fabiani; Marco Saroglia; ...; Fabiana Arduini
journal: Biosensors & bioelectronics
date: 2020-10-03
pmid: 33086175
doi: 10.1016/j.bios.2020.112686
pmcid: 7833515
cited_by_count: 395
techniques: Amplification; Binding Assay; Blocking Assay; CRISPR; Cell Culture; Concentration Assay; Enzyme-linked Immunosorbent Assay; Fluorescence; Immunochromatographic Assay; Incubation; Modification; Negative Control; Produced; Recombinant; Reporter Assay; SPR Assay; Standard Deviation; Transduction
bioz_score: 93
title: A human monoclonal antibody blocking SARS-CoV-2 infection
authors: Chunyan Wang; Wentao Li; ...; Berend-Jan Bosch
journal: Nature Communications
date: 2020-05-04
pmid: 32366817
doi: 10.1038/s41467-020-16256-y
pmcid: 7198537
cited_by_count: 785
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Immunofluorescence; Incubation; Infection; Luciferase; Negative Control; Neutralization; Staining; Strep-tag; Transduction
bioz_score: 93
title: Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient
authors: Jennifer Harcourt; Azaibi Tamin; ...; Natalie J. Thornburg
journal: bioRxiv
date: 2020-03-07
pmid: 32511316
doi: 10.1101/2020.03.02.972935
pmcid: 7239045
cited_by_count: 12
techniques: Adsorption; Concentration Assay; Imaging; Incubation; Infection; Plaque Assay; Quantitation Assay; SDS Page; Western Blot
bioz_score: 93
