| Catalog No. | Size | Price (USD) |
| 40589-T62-B-20 | 20 µg | $177.73 |
| 40589-T62-B-100 | 100 µg | $555.42 |
General Information
| Product name | SARS-CoV-2 Spike Antibody (Biotin), Rabbit PAb, Antigen Affinity Purified |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike S1+S2 ECD |
| Immunogen | Recombinant SARS-CoV-2 / 2019-nCoV Spike Protein (Catalog#40589-V08B1) |
| Preparation | Produced in rabbits immunized with purified, recombinant SARS-CoV-2 / 2019-nCoV Spike Protein (S1+S2 ECD)(Catalog#40589-V08B1; YP_009724390.1; Val16-Pro1213). The specific IgG was purified by SARS-CoV-2 / 2019-nCoV Spike affinity chromatography and then biotinylated. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Biotin |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: A distinctive IGHV3-66 SARS-CoV-2 neutralizing antibody elicited by primary infection with an Omicron variant.
authors: Qing Fan; Congcong Liu; ...; Zheng Zhang
journal: Structure
date: 2025-04-23
pmid: 40306272
doi: 10.1016/j.str.2025.04.005
cited_by_count: 2
techniques: Immunoprecipitation; Incubation; Lysis; SDS Page; Sample Prep; Stable Transfection; Western Blot
bioz_score: 93
title: Serological Test to Determine Exposure to SARS-CoV-2: ELISA Based on the Receptor-Binding Domain of the Spike Protein (S-RBD N318-V510 ) Expressed in Escherichia coli
authors: Alan Roberto Márquez-Ipiña; Everardo González-González; ...; Mario Moisés Alvarez
journal: Diagnostics
date: 2021-02-10
pmid: 33578665
doi: 10.3390/diagnostics11020271
pmcid: 7916330
cited_by_count: 23
techniques: Binding Assay; Incubation
bioz_score: 90
