| Catalog No. | Size | Price (USD) |
| 40591-MM41-B-20 | 20 µg | $296.22 |
| 40591-MM41-B-100 | 100 µg | $888.67 |
General Information
| Product name | SARS-CoV-2 Spike Antibody (Biotin), Mouse MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike S1 |
| Immunogen | Recombinant SARS-CoV-2 Spike Protein (Catalog#40591-V05H1) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV-2 Spike (Catalog#40591-V05H1; YP_009724390.1; Val16-Arg685). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and then biotinylated. |
| Source | Monoclonal Mouse IgG1 Clone #41 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Biotin |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: SARS-CoV-2-derived RNA replicons as safe and effective vaccines
authors: Marta Villarejo-Torres; Iván Nombela-Diaz; ...; Luis Enjuanes
journal: Proceedings of the National Academy of Sciences of the United States of America
date: 2025-09-24
pmid: 40991433
cited_by_count: 0
techniques: Electrophoresis; Incubation; Membrane; Microscopy; Quantitative RT-PCR; Saline; Western Blot
bioz_score: 94
title: Acoustofluidic Virus Isolation via Bessel Beam Excitation Separation Technology
authors: Jianping Xia; Zeyu Wang; ...; Tony Jun Huang
journal: ACS Nano
date: 2024-08-20
pmid: 39132820
cited_by_count: 12
techniques: Digital PCR; Generated
bioz_score: 92
title: An optimized high-throughput SARS-CoV-2 dual reporter trans-complementation system for antiviral screening in vitro and in vivo
authors: Yingjian Li; Xue Tan; ...; Yu Chen
journal: Virologica Sinica
date: 2024-03-26
pmid: 38548102
doi: 10.1016/j.virs.2024.03.009
pmcid: 11280264
cited_by_count: 8
techniques: Agarose Gel Electrophoresis; CCK-8 Assay; Clone Assay; Comparison; Electrophoresis; Expressing; Fluorescence; High Throughput Screening Assay; Imaging; Immunofluorescence; In Vitro; Incubation; Infection; Inhibition; Luciferase; Marker; Membrane; Multiplex Assay; Mutagenesis; Passaging; Plasmid Preparation; Purification; Quantitative RT-PCR; RNA Expression; Reverse Transcription Polymerase Chain Reaction; Sequencing; Western Blot
bioz_score: 92
