| Catalog No. | Size | Price (USD) |
| 40591-T62-H-20 | 20 µg | $177.73 |
| 40591-T62-H-100 | 100 µg | $555.42 |
General Information
| Product name | SARS-CoV-2 Spike Antibody (HRP), Rabbit PAb, Antigen Affinity Purified |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike S1 |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Spike S-His Protein (Catalog#40591-V08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant SARS-CoV-2 (2019-nCoV) Spike S1-His Recombiant Protein (HPLC-verified) (Catalog#40591-V08H; YP_009724390.1; Val16-Arg685). The specific IgG was purified by SARS-CoV-2 / 2019-nCoV Spike affinity chromatography and conjugated with horseradish-peroxidase (HRP). |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | HRP |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: Evaluation of the protective efficacy of a recombinant adenovirus-vectored SARS-CoV-2 vaccine candidate for veterinary use
authors: Chong Wang; Zhiyuan Wen; ...; Zhigao Bu
journal: Frontiers in cellular and infection microbiology
date: 2025-12-17
pmid: 41479544
doi: 10.3389/fcimb.2025.1714427
cited_by_count: 0
techniques: Incubation; Staining; Western Blot
bioz_score: 93
title: Irreversible Inactivation of SARS-CoV-2 by Lectin Engagement with Two Glycan Clusters on the Spike Protein
authors: Aakansha Nangarlia; Farah Fazloon Hassen; ...; Irwin Chaiken
journal: Biochemistry
date: 2023-07-18
pmid: 37341186
doi: 10.1021/acs.biochem.3c00109
pmcid: 10663058
cited_by_count: 6
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Incubation; Infection; Inhibition; Produced; Recombinant
bioz_score: 93
title: Optimization and Deoptimization of Codons in SARS-CoV-2 and Related Implications for Vaccine Development.
authors: Xinkai Wu; Ke-Jia Shan; ...; Jian Lu
journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
date: 2023-06-02
pmid: 37267926
cited_by_count: 21
techniques: Expressing
bioz_score: 93
title: SARS-CoV-2 Delta and Omicron variants evade population antibody response by mutations in a single spike epitope.
authors: Ping He; Banghui Liu; ...; Xiaoli Xiong
journal: Nature Microbiology
date: 2022-09-30
pmid: 36151403
doi: 10.1038/s41564-022-01235-4
cited_by_count: 48
techniques: Blocking Assay; Electron Microscopy; Imaging; Incubation; Membrane; Software; Transmission Electron Microscopy; Western Blot
bioz_score: 93
