| Catalog No. | Size | Price (USD) |
| 40592-MM57-20 | 20 µg | $296.22 |
| 40592-MM57-100 | 100 µg | $592.44 |
General Information
| Product name | SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse Mab |
| Validated applications | ELISA (FAQ Protocol), FCM (FAQ Protocol), ICC/IF (FAQ Protocol), Neutralization |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike RBD Protein (Could't block ACE2) Has cross-reactivity in ELISA with SARS-CoV-2 Omicron (BA.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H29) SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H10) SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 Protein (Cat# 40589-V08B16) SARS-CoV-2 Delta (B.1.617.2) Spike S1 Protein (Cat# 40591-V08H23) SARS-CoV-2 Delta (B.1.617.2) Spike RBD Protein (Cat# 40592-V08H90) SARS-CoV-2 Spike RBD (K417T, E484K, N501Y) Protein (Cat# 40592-V08H86) SARS-CoV-2 Spike RBD (N501Y) Protein (Cat# 40592-V08H82) SARS-CoV-2 Spike S1 Protein (Catalog#40591-V08B1) SARS-CoV-2 Spike RBD Protein (Catalog#40592-V08B) Medium cross-reactivity in ELISA with SARS-CoV-2 Omicron (BA.1.1) Spike RBD Protein (Cat# 40592-V08H129) Minimal cross-reactivity in ELISA with SARS-CoV-2 Spike RBD (K417N, E484K, N501Y) Protein (Cat# 40592-V08H85) No cross-reactivity in ELISA with SARS-CoV-2 Omicron (XBB.1.16) Spike S1+S2 trimer Protein (Cat# 40589-V08H48) SARS-CoV-2 Omicron (XBB.1.5) Spike RBD Protein (Cat# 40592-V08H146) SARS-CoV-2 Omicron (XBB.1.5) Spike S1+S2 trimer Protein (Cat# 40589-V08H45) SARS-CoV-2 Omicron (XBB.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H42) SARS-CoV-2 Omicron (XBB) Spike RBD Protein (Cat# 40592-V08H144) SARS-CoV-2 Omicron (BQ.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H41) SARS-CoV-2 Omicron (BQ.1.1) Spike RBD Protein (Cat# 40592-V08H143) SARS-CoV-2 Omicron (BF.7) Spike S1+S2 trimer Protein (Cat# 40589-V08H39) SARS-CoV-2 Omicron (BA.4.6/BF.7) Spike RBD Protein (Cat# 40592-V08H140) SARS-CoV-2 Omicron (BA.4.6) Spike S1+S2 trimer Protein (Cat# 40589-V08H37) SARS-CoV-2 Omicron (BA.2.75) Spike RBD Protein (Cat# 40592-V08H135) SARS-CoV-2 Omicron (BA.2.75) Spike S1+S2 trimer Protein (Cat# 40589-V08H36) SARS-CoV-2 Omicron (BA.2.75.2) Spike RBD Protein (Cat# 40592-V08H141) SARS-CoV-2 Omicron (BA.2.75.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H38) SARS-CoV-2 Omicron (BA.2.3.20) Spike RBD Protein (Cat# 40592-V08H142) SARS-CoV-2 Omicron (B.1.1.529) S1+S2 trimer Protein (Cat# 40589-V08H26) SARS-CoV-2 Omicron (B.1.1.529) Spike S1 Protein (Cat# 40591-V08H41) SARS-CoV-2 Omicron (B.1.1.529) Spike RBD Protein (Cat# 40592-V08H121) SARS-CoV-2 Omicron (BA.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H28) SARS-CoV-2 Omicron (BA.2) Spike S1 Protein (Cat# 40591-V08H43) SARS-CoV-2 Omicron (BA.2) Spike RBD Protein (Cat# 40592-V08H123) SARS-CoV-2 Omicron (BA.2) Spike S1 NTD Protein (Cat# 40591-V08H44) SARS-CoV-2 Omicron (BA.2.12.1) Spike RBD Protein (Cat# 40592-V08H132) SARS-CoV-2 Omicron (BA.4/BA.5/BA.5.2) Spike RBD Protein (Cat# 40592-V08H130) SARS-CoV-2 Omicron (BA.5) Spike RBD Protein (Cat# 40592-V08H131) SARS-CoV-2 XD(BA.1 x AY.4) Spike S1+S2 trimer Protein (Cat# 40589-V08H30) SARS-CoV-2 Delta (B.1.617.2) Spike S1 NTD Protein (Cat# 40591-V08H36) SARS-CoV Spike S1-mFc Protein (Catalog#40150-V08B1) SARS-CoV Spike RBD-His Protein (Catalog#40150-V08B2) MERS-CoV Spike S1 Protein (Cat# 40069-V08B1) HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H) HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H) HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H) HCoV-229E Spike S1 Protein (Cat# 40601-V08H) HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B) |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H; YP_009724390.1; Arg319-Phe541). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2b Clone #57 |
| Purification | Protein A |
| Formulation | 25 mM Sodium Citrate, 200 mM NaCl, 0.02% Tween-80, pH 6.0 |
| Conjugate | Unconjugated |
| Endotoxin | < 3 EU/mg |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-MM57). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 (WT) is typically 0.41 µg/mL. |
| Image 1 Alt Text | 2019-nCoV Coronavirus spike Microneutralizaiton (MN) 30080 |
| Image 1 URL | Source image |
| Image 2 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-MM57). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 (WT) is typically 0.41 µg/mL. |
| Image 2 Alt Text | SARS-CoV-2 Coronavirus spike Flow Cytometry (FC) 32516 |
| Image 2 URL | Source image |
| Image 3 Description | The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 Spike Pseudovirus (WT, Cat# PSV001) under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-MM57). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC 50 (WT) is typically 0.41 µg/mL. |
| Image 3 Alt Text | SARS-CoV-2 Coronavirus spike Immunofluorescence(IF) 32693 |
| Image 3 URL | Source image |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
[{"title":"Cell-free expression of antibodies, antigen-binding fragments thereof, and antibody derivatives","authors":["Bastian Vogeli","Michael Christopher Jewett","Andrew Colin Hunt"],"journal":"","date":"2026-07-07","pmid":"","doi":"","pmcid":"","cited_by_count":0,"techniques":["Concentration Assay","Enzyme-linked Immunosorbent Assay","Incubation"],"bioz_score":"95"},{"title":"Structure-Guided Design of Proteomimetics Targeting the SARS-CoV‑2 S‑RBD/hACE2 Interface","authors":["Sára Ferková","Agathe Fayolle","...","Pierre-Luc Boudreault"],"journal":"Journal of Medicinal Chemistry","date":"2026-06-15","pmid":"42289940","doi":"10.1021/acs.jmedchem.5c03450","pmcid":"","cited_by_count":0,"techniques":["Cell Culture","In Vitro","Produced"],"bioz_score":"93"},{"title":"Quantum dot conjugated virus spike protein for cell-based bio-sensing systems and drug screening","authors":["Eunkeu Oh","Kimihiro Susumu","...","Kirill Gorshkov"],"journal":"","date":"2026-03-31","pmid":"","doi":"","pmcid":"","cited_by_count":0,"techniques":["Electron Microscopy","High Content Screening","Modification","Plasmid Preparation"],"bioz_score":"95"},{"title":"Interferon-inducible guanylate-binding protein 5 inhibits replication of multiple viruses by binding to the oligosaccharyltransferase complex and inhibiting glycoprotein maturation.","authors":["Shaobo Wang","Wanyu Li","...","Tariq M Rana"],"journal":"mBio","date":"2025-11-17","pmid":"41247044","doi":"10.1128/mbio.02930-25","pmcid":"","cited_by_count":1,"techniques":["Immunofluorescence","Microscopy"],"bioz_score":"95"},{"title":"Recombinant severe respiratory syndrome coronavirus 2 Rbd trimer protein vaccine capable of generating broad-spectrum cross neutralization activity, and preparation method and use thereof","authors":["Qiming Li","Yu Liang","...","Ning Liu"],"journal":"","date":"2025-06-10","pmid":"","doi":"","pmcid":"","cited_by_count":0,"techniques":["Activity Assay","Binding Assay","Bioprocessing","Concentration Assay","Enzyme-linked Immunosorbent Assay","Expressing","Incubation","Mutagenesis","Negative Control","Purification","Recombinant","Sequencing","Virus"],"bioz_score":"94"},{"title":"Magnetic Bead-Based Electrochemical Surrogate Virus Neutralization Test for Quantification of Antibody Neutralizing Efficiency","authors":["Rae A. 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Gilmore","...","Prasenjit K. Mukherjee"],"journal":"","date":"2024-02-13","pmid":"","doi":"","pmcid":"","cited_by_count":0,"techniques":["Binding Assay","Membrane"],"bioz_score":"95"},{"title":"Discovering covalent inhibitors of protein-protein interactions from trillions of sulfur(VI) fluoride exchange-modified oligonucleotides.","authors":["Zichen Qin","Kaining Zhang","...","Yu Xiang"],"journal":"Nature Chemistry","date":"2023-12-06","pmid":"37653229","doi":"10.1038/s41557-023-01304-z","pmcid":"","cited_by_count":2,"techniques":["Luciferase","Modification","Neutralization","Sequencing","Synthesized"],"bioz_score":"95"},{"title":"Wireless, battery-free, multifunctional integrated bioelectronics for respiratory pathogens monitoring and severity evaluation.","authors":["Hu Li","Huarui Gong","...","Xinge Yu"],"journal":"Nature Communications","date":"2023-11-22","pmid":"37985765","doi":"10.1038/s41467-023-43189-z","pmcid":"","cited_by_count":4,"techniques":["Virus"],"bioz_score":"95"},{"title":"F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells","authors":["Jitendriya Swain","Peggy Merida","...","Delphine Muriaux"],"journal":"iScience","date":"2023-07-17","pmid":"","doi":"10.1016/j.isci.2023.107384","pmcid":"10410521","cited_by_count":6,"techniques":["Recombinant","Software"],"bioz_score":"94"},{"title":"A rapid cell-free expression and screening platform for antibody discovery.","authors":["Andrew C Hunt","Bastian Vögeli","...","Michael C Jewett"],"journal":"Nature Communications","date":"2023-07-05","pmid":"37400446","doi":"","pmcid":"","cited_by_count":31,"techniques":["Concentration Assay","Enzyme-linked Immunosorbent Assay","Incubation"],"bioz_score":"94"},{"title":"Inactivated and Immunogenic SARS-CoV-2 for Safe Use in Immunoassays and as an Immunization Control for Non-Clinical Trials","authors":["Mariana Pierre de Barros Gomes","José Henrique Rezende Linhares","...","Ronald N. Harty"],"journal":"Viruses","date":"2023-06-30","pmid":"37515173","doi":"","pmcid":"10386713","cited_by_count":5,"techniques":["Bicinchoninic Acid Protein Assay","Comparison","Enzyme-linked Immunosorbent Assay","Functional Assay","Incubation","Produced","Protein Concentration","Sandwich ELISA","Virus"],"bioz_score":"94"},{"title":"Cellular electrical impedance to profile SARS-CoV-2 fusion inhibitors and to assess the fusogenic potential of spike mutants.","authors":["Emiel Vanhulle","Jordi Doijen","...","Kurt Vermeire"],"journal":"Antiviral research","date":"2023-04-27","pmid":"36977434","doi":"10.1016/j.antiviral.2023.105587","pmcid":"","cited_by_count":6,"techniques":["Binding Assay","Biomarker Discovery","Cell-Cell Fusion Assay","Comparison","Expressing","Flow Cytometry","Membrane","Transfection"],"bioz_score":"95"},{"title":"Rapid, Sensitive, Label-Free Electrical Detection of SARS-CoV-2 in Nasal Swab Samples","authors":["Hyun-June Jang","Wen Zhuang","...","Junhong Chen"],"journal":"ACS Applied Materials & Interfaces","date":"2023-03-20","pmid":"36938607","doi":"10.1021/acsami.3c00331","pmcid":"10041344","cited_by_count":2,"techniques":["Enzyme-linked Immunosorbent Assay","Incubation","Infection"],"bioz_score":"94"},{"title":"The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interaction.","authors":["Youngwook Ham","Nam-Chul Cho","...","Sungchan Cho"],"journal":"Biosensors & bioelectronics","date":"2023-03-14","pmid":"36827795","doi":"","pmcid":"","cited_by_count":3,"techniques":["Incubation"],"bioz_score":"94"},{"title":"A SARS-CoV-2 vaccine designed for manufacturability results in unexpected potency and non-waning humoral response","authors":["Campbell Elliot","Dobkin Julie","...","Anderson Stephen"],"journal":"bioRxiv","date":"2023-02-07","pmid":"","doi":"10.1101/2023.02.06.527376","pmcid":"","cited_by_count":1,"techniques":["Activity Assay","Control","Enzyme-linked Immunosorbent Assay"],"bioz_score":"94"},{"title":"SARS-CoV-2 spike HexaPro formulated in aluminium hydroxide and administered in an accelerated vaccination schedule partially protects Syrian Hamsters against viral challenge despite low neutralizing antibody responses","authors":["Dennis Christensen","Charlotta Polacek","...","Gabriel Kristian Pedersen"],"journal":"Frontiers in Immunology","date":"2023-01-23","pmid":"","doi":"10.3389/fimmu.2023.941281","pmcid":"9900178","cited_by_count":3,"techniques":["Activity Assay","Binding Assay","Competitive Binding Assay","Construct","Derivative Assay","Enzyme-linked Immunosorbent Assay","Incubation","Infection","Inhibition","Neutralization","Positive Control","Staining"],"bioz_score":"94"},{"title":"Inhibitory activities of alginate phosphate and sulfate derivatives against SARS-CoV-2 in vitro.","authors":["Cheng Yang","Dan Li","...","Chunxia Li"],"journal":"International Journal of Biological Macromolecules","date":"2023-01-12","pmid":"36481336","doi":"10.1016/j.ijbiomac.2022.11.311","pmcid":"","cited_by_count":4,"techniques":["Activity Assay"],"bioz_score":"94"},{"title":"Understanding the Spike Protein in COVID-19 Vaccine in Recombinant Vesicular Stomatitis Virus (rVSV) Using Automated Capillary Western Blots","authors":["Paul F. Gillespie","Yanjie Wang","...","Richard R. Rustandi"],"journal":"ACS Omega","date":"2023-01-10","pmid":"","doi":"10.1021/acsomega.2c06937","pmcid":"9843631","cited_by_count":0,"techniques":["Concentration Assay","Generated"],"bioz_score":"94"},{"title":"An inactivated SARS-CoV-2 vaccine induced cross-neutralizing persisting antibodies and protected against challenge in small animals","authors":["Anna Offersgaard","Carlos Rene Duarte Hernandez","...","Judith Margarete Gottwein"],"journal":"iScience","date":"2023-01-10","pmid":"36644321","doi":"","pmcid":"9829433","cited_by_count":6,"techniques":["Adjuvant","Bicinchoninic Acid Protein Assay","Binding Assay","Clinical Proteomics","Comparison","Double-Color Enzymatic ELISPOT","Enzyme-linked Immunospot","In Vitro","MANN-WHITNEY","Neutralization","Recombinant","Sampling","Software","Virus","dsDNA Assay"],"bioz_score":"95"},{"title":"The rapid and highly parallel identification of antibodies with defined biological activities by SLISY","authors":["Steve Lu","Austin K. Mattox","...","Kenneth W. Kinzler"],"journal":"Nature Communications","date":"2023-01-03","pmid":"36596784","doi":"10.1038/s41467-022-35668-6","pmcid":"9808734","cited_by_count":4,"techniques":["Activity Assay","Binding Assay","Blocking Assay","Clone Assay","Enzyme-linked Immunosorbent Assay","Expressing","Incubation","Infection","Luciferase","Negative Control","Neutralization","Positive Control","Recombinant","Sequencing","Standard Deviation","Variant Assay"],"bioz_score":"94"},{"title":"In Vitro Diagnostic Assay to Detect SARS-CoV-2-Neutralizing Antibody in Patient Sera Using Engineered ACE-2 Mini-Protein","authors":["Bruna Andersen Pereira de Jesus","Anderson Albino Gomes","...","Daniele Focosi"],"journal":"Viruses","date":"2022-12-18","pmid":"36560827","doi":"10.3390/v14122823","pmcid":"9780992","cited_by_count":3,"techniques":["Incubation","Neutralization"],"bioz_score":"94"},{"title":"Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivo","authors":["James A. Torchia","Alexander H. Tavares","...","Gordon J. 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