| Catalog No. | Size | Price (USD) |
| 40592-R118-B-20 | 20 µg | $296.22 |
| 40592-R118-B-100 | 100 µg | $888.67 |
General Information
| Product name | SARS-CoV-2 Spike Antibody (Biotin), Rabbit Mab |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike RBD Protein (Can block the binding between RBD and ACE2) |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H) |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells and then biotinylated. |
| Source | Monoclonal Rabbit IgG Clone #118 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Biotin |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
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title: A label-free aptasensing method for detecting SARS-CoV-2 virus antigen by using dumbbell probe-mediated circle-to-circle amplification.
authors: Zecheng Wang; Si He; ...; Danke Xu
journal: Analytical and Bioanalytical Chemistry
date: 2024-03-01
pmid: 38349532
doi: 10.1007/s00216-024-05195-y
cited_by_count: 1
techniques: Binding Assay; Magnetic Beads; Recombinant; SYBR Green Assay
bioz_score: 92
title: Safety and immunogenicity of inactivated SARS-CoV-2 vaccines in people living with HIV
authors: Ling Ao; Ting Lu; ...; Hong Ren
journal: Emerging microbes & infections
date: 2022-04-18
pmid: 35369854
doi: 10.1080/22221751.2022.2059401
pmcid: 9037169
cited_by_count: 47
techniques: Binding Assay; Flow Cytometry; Gradient Centrifugation; Isolation; Staining
bioz_score: 91
title: SARS-CoV-2 Proteins Microarray by Photoimmobilization for Serodiagnosis of the Antibodies
authors: Hiroharu Kashiwagi; Nobuhiro Morishima; ...; Yoshihiro Ito
journal: Bulletin of the Chemical Society of Japan
date: 2021-09-03
doi: 10.1246/bcsj.20210215
cited_by_count: 0
techniques: Control; Negative Control
bioz_score: 92
title: SARS-CoV-2 Rapidly Adapts in Aged BALB/c Mice and Induces Typical Pneumonia
authors: Yufei Zhang; Kun Huang; ...; Meilin Jin
journal: Journal of virology
date: 2021-05-10
doi: 10.1128/jvi.02477-20
cited_by_count: 40
techniques: Incubation; Microscopy; Protein Binding
bioz_score: 90
title: Global Absence and Targeting of Protective Immune States in Severe COVID-19.
authors: Alexis J. Combes; Tristan Courau; ...; Matthew F. Krummel
journal: Research Square
date: 2020-10-28
pmid: 33140041
doi: 10.21203/rs.3.rs-97042/v1
pmcid: 7605560
cited_by_count: 0
techniques: Infection; Staining
bioz_score: 92
