| Catalog No. | Size | Price (USD) |
| 40592-R505-20 | 20 µg | $296.22 |
| 40592-R505-100 | 100 µg | $592.44 |
General Information
| Product name | SARS-CoV-2 Spike Neutralizing Antibody, Rabbit MAb |
| Validated applications | ELISA (FAQ Protocol), Neutralization |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike RBD Protein (Could't block the binding between RBD and ACE2) Has cross-reactivity in ELISA with SARS-CoV-2 Omicron (BA.2.75) Spike RBD Protein (Cat# 40592-V08H135) SARS-CoV-2 Omicron (BA.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H29) SARS-CoV-2 Omicron (BA.1.1) Spike RBD Protein (Cat# 40592-V08H129) SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H10) SARS-CoV-2 Delta (B.1.617.2) Spike S1 Protein (Cat# 40591-V08H23) SARS-CoV-2 Delta (B.1.617.2) Spike RBD Protein (Catalog#40592-V49H1-B) SARS-CoV-2 Spike RBD Protein (WT, no-variants) (Catalog#40592-V08H, 40592-V08B, 40592-V27H-B) Medium cross-reactivity in ELISA with SARS-CoV-2 Omicron (XBB) Spike RBD Protein (Cat# 40592-V08H144) SARS-CoV-2 Omicron (BQ.1.1) Spike RBD Protein (Cat# 40592-V08H143) SARS-CoV-2 Omicron (BA.4.6/BF.7) Spike RBD Protein (Cat# 40592-V08H140) SARS-CoV-2 Omicron (BA.2.75.2) Spike RBD Protein (Cat# 40592-V08H141) SARS-CoV-2 Omicron (BA.2.3.20) Spike RBD Protein (Cat# 40592-V08H142) No cross-reactivity in ELISA with SARS-CoV-2 Omicron (XBB.1.16) Spike S1+S2 trimer Protein (Cat# 40589-V08H48) SARS-CoV-2 Omicron (XBB.1.5) Spike RBD Protein (Cat# 40592-V08H146) SARS-CoV-2 Omicron (XBB.1.5) Spike S1+S2 trimer Protein (Cat# 40589-V08H45) SARS-CoV-2 Omicron (XBB.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H42) SARS-CoV-2 Omicron (BQ.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H41) SARS-CoV-2 Omicron (BF.7) Spike S1+S2 trimer Protein (Cat# 40589-V08H39) SARS-CoV-2 Omicron (BA.4.6) Spike S1+S2 trimer Protein (Cat# 40589-V08H37) SARS-CoV-2 Omicron (BA.2.75) Spike S1+S2 trimer Protein (Cat# 40589-V08H36) SARS-CoV-2 Omicron (BA.2.75.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H38) SARS-CoV-2 Omicron (B.1.1.529) Spike RBD Protein (Catalog#40592-V49H7) SARS-CoV-2 Omicron (BA.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H28) SARS-CoV-2 Omicron (BA.2) Spike S1 Protein (Cat# 40591-V08H43) SARS-CoV-2 Omicron (BA.2) Spike RBD Protein (Cat# 40592-V08H123) SARS-CoV-2 Omicron (BA.2) Spike S1 NTD Protein (Cat# 40591-V08H44) SARS-CoV-2 Omicron (BA.2.12.1) Spike RBD Protein (Cat# 40592-V08H132) SARS-CoV-2 Omicron (BA.4/BA.5/BA.5.2) Spike RBD Protein (Cat# 40592-V08H130) SARS-CoV-2 Omicron (BA.5) Spike RBD Protein (Cat# 40592-V08H131) SARS-CoV-2 XD(BA.1 x AY.4) Spike S1+S2 trimer Protein (Cat# 40589-V08H30) SARS-CoV-2 Delta (B.1.617.2) Spike S1 NTD Protein (Cat# 40591-V08H36) |
| Immunogen | Recombinant SARS-CoV-2 Spike RBD Protein (Catalog#40592-V05H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant SARS-CoV-2 Spike RBD-mFc Protein (Catalog#40592-V05H; YP_009724390.1; Arg319-Phe541). |
| Source | Monoclonal Rabbit IgG Clone #505 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | The neutralization activity is measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 Spike Pseudovirus (WT, Cat# PSV001), or SARS-CoV-2 Spike Omicron (B.1.1.529) Pseudovirus (Cat# PSV016), or SARS-CoV-2 Delta (B.1.617.2) variant Spike Pseudovirus (Cat# PSV011), under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-R505). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. IC 50 (WT)is 11.74 µg/mL. No neutralization activity to SARS-CoV-2 Delta (B.1.617.2) variant Spike Pseudovirus (Cat# PSV011), and SARS-CoV-2 Omicron (B.1.1.529) variant Spike Pseudovirus (Cat# PSV016). |
| Image 1 Alt Text | SARS-CoV-2 Coronavirus spike Microneutralizaiton (MN) 33800 |
| Image 1 URL | Source image |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
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techniques: Inhibition; Virus
bioz_score: 93
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bioz_score: 93
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techniques: Infection; Labeling; Software; Staining
bioz_score: 93
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journal: bioRxiv
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techniques: Expressing; Immunohistochemistry; Incubation; Infection; Injection; Labeling; Multiplex Assay; Neutralization; Plaque Assay; Positive Control; RNA Sequencing Assay; Software; Staining
bioz_score: 93
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techniques: Activity Assay; Bicinchoninic Acid Protein Assay; Incubation; Infection; Neutralization; Plaque Assay
bioz_score: 93
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techniques: Immunostaining; Incubation; Infection; Software; Staining; Standard Deviation
bioz_score: 93
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techniques: Immunostaining; Incubation; Infection; Software; Staining
bioz_score: 93
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techniques: Imaging; Incubation; Saline; Software; Staining
bioz_score: 94
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pmcid: 9302186
cited_by_count: 219
techniques: Binding Assay; Blocking Assay; Positive Control
bioz_score: 94
title: Homologous or heterogenous vaccination boosters enhance neutralizing activities against SARS‐CoV‐2 Omicron BA.1 variant
authors: Zhongcheng Zhou; Peng Du; ...; Gen Li
journal: MedComm
date: 2022-05-11
doi: 10.1002/mco2.143
pmcid: 9095991
cited_by_count: 2
techniques: Activity Assay; Binding Assay; Blocking Assay; Derivative Assay; Expressing; Infection; Inhibition; Mutagenesis; Neutralization; Produced; Recombinant; Serial Dilution; Stable Transfection; Variant Assay
bioz_score: 93
title: Biofunctionalization of Graphene‐Based FET Sensors through Heterobifunctional Nanoscaffolds: Technology Validation toward Rapid COVID‐19 Diagnostics and Monitoring
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techniques: Modification; Recombinant
bioz_score: 93
title: Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 Expression
authors: Mariano Carossino; Devin Kenney; ...; Herve J A Fleury
journal: Viruses
date: 2022-03-05
pmid: 35336942
doi: 10.3390/v14030535
pmcid: 8955233
cited_by_count: 71
techniques: Activity Assay; Electron Microscopy; Expressing; Immunohistochemistry; Incubation; Infection; Injection; Isolation; Labeling; Multiplex Assay; Optical Imaging; Positive Control; Reverse Transcription Polymerase Chain Reaction; Software; Transmission Assay
bioz_score: 93
title: Rapid biosensing SARS-CoV-2 antibodies in vaccinated healthy donors
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journal: Biosensors & bioelectronics
date: 2022-02-03
pmid: 35151002
doi: 10.1016/j.bios.2022.114054
pmcid: 8810518
cited_by_count: 25
techniques: Amplification; Bicinchoninic Acid Protein Assay; Binding Assay; Conjugation Assay; Enzyme-linked Immunosorbent Assay; Inhibition; Injection; Labeling; Mutagenesis; Neutralization; Produced; Recombinant; Synthesized; Titer Assay
bioz_score: 93
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doi: 10.1101/2021.08.08.455272
cited_by_count: 0
techniques: Incubation; Isolation; Staining
bioz_score: 93
