| Catalog No. | Size | Price (USD) |
| 68088-MM05-20 | 20 µL | $148.11 |
| 68088-MM05-100 | 100 µL | $473.95 |
General Information
| Product name | HCoV-HKU1 Spike Antibody, Mouse MAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | Recognize the Spike of HCoV-HKU1 (isolate N5) in ELISA. Don't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-229E, HCoV-OC43. Has cross-reactivity in ELISA and WB with HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His (Cat# 40606-V08B) No cross-reactivity in ELISA and WB with SARS-CoV-2 Spike S1+S2 ECD-His (Cat# 40589-V08B1) SARS-CoV-2 Spike S2 ECD-His (Cat# 40590-V08B) SARS-CoV-2 Spike S1-His (Cat# 40591-V08H) SARS-CoV-2 Spike S1 NTD-Fc & AVI (Cat# 40591-V41H) SARS-CoV-2 Spike S1 NTD-His & AVI (Cat# 40591-V49H) SARS-CoV-2 Spike RBD-His (Cat# 40592-V08B) SARS-CoV Spike S1+S2 ECD-His (Cat# 40634-V08B) MERS-CoV Spike S1+S2 ECD-His (Cat# 40069-V08B) HCoV-NL63 Spike S1+S2 ECD-His (Cat# 40604-V08B) HCoV-229E Spike S1+S2 ECD-His (Cat# 40605-V08B) HCoV-OC43 Spike S1+S2 ECD-His (Cat# 40607-V08B) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant CoV spike S1+S2 ECD protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #05 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-HCoV-HKU1 Spike mouse monoclonal antibody at 1:1000 dilution. Sample: Recombinant Protein 10 ng Lane 1: SARS-CoV-2 Spike S1-His (Cat#40591-V08H) Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI (Cat#40591-V41H) Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI (Cat#40591-V49H) Lane 4: SARS-CoV-2 Spike RBD-His (Cat#40592-V08B) Lane 5: SARS-CoV-2 Spike S2 ECD-His (Cat#40590-V08B) Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His (Cat#40589-V08B1) Lane 7: SARS-CoV Spike S1+S2 ECD-His (Cat#40634-V08B) Lane 8: MERS-CoV Spike S1+S2 ECD-His (Cat#40069-V08B) Lane 9: HCoV-NL63 Spike S1+S2 ECD-His (Cat#40604-V08B) Lane 10: HCoV-229E Spike S1+S2 ECD-His (Cat#40605-V08B) Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His (Cat#40606-V08B) Lane 12: HCoV-OC43 Spike S1+S2 ECD-His (Cat#40607-V08B) Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | other Coronavirus spike Western blot (WB) 33778 |
| Image 1 URL | Source image |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: Cellular Immune Responses to SARS-CoV-2 in Exposed Seronegative Individuals
authors: Natasha J. Norton; Kayla A. Holder; ...; Yinzhong Shen
journal: Viruses
date: 2023-04-18
pmid: 37112977
doi: 10.3390/v15040996
pmcid: 10144856
cited_by_count: 8
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; MANN-WHITNEY; Recombinant
bioz_score: 91
