WB (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human CRKL
Immunogen
E. coli-derived Human CRKL fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human CRKL fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
Anti-CRKL rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:37 kDa
Anti-CRKL rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:37 kDa
Anti-CRKL rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:37 kDa
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