| Catalog No. | Size | Price (USD) |
| 201778-T46-50 | 50 µL | $147.96 |
| 201778-T46-100 | 100 µL | $251.64 |
| 201778-T46-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-ADD1/adducin 1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ADD1/adducin 1 |
| Immunogen | E. coli-derived Human ADD1/adducin 1 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human ADD1/adducin 1 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ADDA Antibody
Image / Experimental Information
| Image 1 Description | Anti-ADD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:81 kDa |
| Image 1 Alt Text | Human ADD1/adducin 1 Western blot (WB) 29254 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ADD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:81 kDa |
| Image 2 Alt Text | Human ADD1/adducin 1 Immunohistochemistry(IHC) 29255 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-ADD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:81 kDa |
| Image 3 Alt Text | Human ADD1/adducin 1 Immunofluorescence(IF) 29256 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-ADD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:81 kDa |
| Image 4 Alt Text | Human ADD1/adducin 1 Immunoprecipitation(IP) 29257 |
| Image 4 URL | Source image |
Background Information
| Full Name | adducin 1 (alpha) |
| Description | The alpha-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adipocyte determination- and differentiation-dependent factor 1 (ADD1) plays important roles in lipid metabolism and insulin-dependent gene expression. Because insulin stimulates carbohydrate and lipid synthesis, it would be important to decipher how the transcriptional activity of ADD1/SREBP1c is regulated in the insulin signaling pathway. Adipocyte determination and differentiation dependent factor 1 (ADD1)/sterol regulatory element binding protein isoform (SREBP1c) is a key transcription factor in fatty acid metabolism and insulin- dependent gene expression. Adipocyte determination and differentiation-dependent factor 1 (ADD1) is a member of the basic helix-loop-helix leucine zipper (bHLH-LZ) family of transcription factors that binds to two distinct DNA sequences and has been associated with both adipocyte development and cholesterol homeostasis (where it has been termed SREBP1). |
-HD12SE0649-WB.png){kind=link}
-HD12SE0649-2000-human-kidney-IHC-P.png){kind=link}
-HD12SE0649-200-HeLa-IF.png){kind=link}
