| Catalog No. | Size | Price (USD) |
| 90001-MM04-1 | 1 mL | $1,377.43 |
| 90001-MM04-20 | 20 µL | $88.87 |
| 90001-MM04-50 | 50 µL | $147.96 |
| 90001-MM04-100 | 100 µL | $251.64 |
| 90001-MM04-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-p53 Antibody, Mouse Monoclonal |
| Validated applications | ELISA(Cap) (FAQ Protocol) |
| Species reactivity | Reacts with: Cynomolgus |
| Specificity | Cynomolgus p53 |
| Immunogen | Recombinant Cynomolgus p53 Protein (Catalog#90001-CNAE) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus p53 (Catalog#90001-CNAE; E3U906; Met1-Asp393). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #04 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Full Name | tumor protein p53 |
| Description | p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitin ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma. |
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| References |
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title: Replication-competent influenza virus with a protein-responsive multiplication ability.
authors: Shinzi Ogasawara
journal: New Biotechnology
date: 2023-09-27
pmid: 37586547
doi: 10.1016/j.nbt.2023.08.001
cited_by_count: 0
techniques: Blocking Assay; Fluorescence; Incubation; Membrane; Staining; Western Blot
bioz_score: 92
title: Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells
authors: Zhaoyu Du; Shuanshuan Xu; ...; Sha Peng
journal: Cell death & disease
date: 2018-09-20
pmid: 30237484
doi: 10.1038/s41419-018-0956-4
pmcid: 6148071
cited_by_count: 32
techniques: Bicinchoninic Acid Protein Assay; BrdU Incorporation Assay; Cell Culture; Expressing; Flow Cytometry; Imaging; Immunofluorescence; Quantitative RT-PCR; TUNEL Assay; Western Blot
bioz_score: 90
