| Catalog No. | Size | Price (USD) |
| 202536-T38-50 | 50 µL | $147.96 |
| 202536-T38-100 | 100 µL | $251.64 |
| 202536-T38-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-DDX21 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human DDX21 |
| Immunogen | E. coli-derived Human DDX21 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human DDX21 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-gu-alpha Antibody; Anti-GUA Antibody; Anti-GURDB Antibody; Anti-II/Gu Antibody; Anti-RH Antibody; Anti-RH II/Gu Antibody; Anti-RH-II/GU Antibody
Image / Experimental Information
| Image 1 Description | Anti-DDX21 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:87 kDa Observed band size:95 kDa |
| Image 1 Alt Text | Human DDX21 Western blot (WB) 22459 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-DDX21 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:87 kDa Observed band size:95 kDa |
| Image 2 Alt Text | Human DDX21 Immunofluorescence(IF) 22460 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-DDX21 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:87 kDa Observed band size:95 kDa |
| Image 3 Alt Text | Human DDX21 Immunoprecipitation(IP) 22461 |
| Image 3 URL | Source image |
Background Information
| Description | DEAD-box RNA helicases are vital for the regulation of various aspects of the RNA life cycle. The DEAD-box RNA helicase DDX21 can sense the transcriptional status of both RNA polymerase (Pol) I and II to control multiple steps of ribosome biogenesis in human cells. DDX21 widely associates with Pol I- and Pol II-transcribed genes and with diverse species of RNA, most prominently with non-coding RNAs involved in the formation of ribonucleoprotein complexes, including ribosomal RNA, small nucleolar RNAs (snoRNAs) and 7SK RNA. In the nucleolus, DDX21 occupies the transcribed rDNA locus, directly contacts both rRNA and snoRNAs, and promotes rRNA transcription, processing and modification. In the nucleoplasm, DDX21 binds 7SK RNA and, as a component of the 7SK small nuclear ribonucleoprotein (snRNP) complex, is recruited to the promoters of Pol II-transcribed genes encoding ribosomal proteins and snoRNAs. Promoter-bound DDX21 facilitates the release of the positive transcription elongation factor b (P-TEFb) from the 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. |
| References |
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