| Catalog No. | Size | Price (USD) |
| 15393-T52-50 | 50 µL | $147.96 |
| 15393-T52-100 | 100 µL | $251.64 |
General Information
| Product name | Anti-ADPRH Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ADPRH |
| Immunogen | Recombinant Human ADPRH Protein (Catalog#15393-H07E) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human ADPRH (rh ADPRH; Catalog#15393-H07E; NP_001116.1; Met1-Leu351). ADPRH specific IgG was purified by Human ADPRH affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ARH1 Antibody; Anti-hARH1 Antibody
Image / Experimental Information
| Image 1 Description | Anti-ADPRH rabbit polyclonal antibody at 1:500 dilution Lane A: Hela whole cell lysate Lane A: K562 whole cell lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:39 kDa |
| Image 1 Alt Text | Human ADPRH Western blot (WB) 35807 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ADPRH rabbit polyclonal antibody at 1:500 dilution Lane A: Hela whole cell lysate Lane A: K562 whole cell lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:39 kDa |
| Image 2 Alt Text | Human ADPRH Immunoprecipitation(IP) 35808 |
| Image 2 URL | Source image |
Background Information
| Full Name | ADP-ribosylarginine hydrolase |
| Description | Cholera toxin (CT) produced by Vibrio cholerae causes the devastating diarrhea of cholera by catalyzing the ADP-ribosylation of the alpha subunit of the intestinal Gs protein (Gsalpha), leading to characteristic water and electrolyte losses. Mammalian cells contain ADP-ribosyltransferases similar to CT and an ADP-ribosyl(arginine)protein hydrolase (ADPRH), which cleaves the ADP-ribose-(arginine)protein bond, regenerating native protein and completing an ADP-ribosylation cycle. CT-catalyzed ADP-ribosylation of cell proteins can be counteracted by ADPRH, which could function as a modifier gene in disease. Further, our study demonstrates that enzymatic cross talk exists between bacterial toxin ADP-ribosyltransferases and host ADP-ribosylation cycles. In disease, toxin-catalyzed ADP-ribosylation overwhelms this potential host defense system, resulting in persistence of ADP-ribosylation and intoxication of the cell. Mono-ADP-ribosylation is a reversible modification of proteins with NAD:arginine ADP-ribosyltransferases and ADP-ribosylarginine hydrolases (ADPRH) catalyzing the opposing arms of an ADP-ribosylation cycle. The ADPRH cDNA had been cloned from human, rat, and mouse tissues and high levels of mRNA were found in brain, spleen, and testis. Human ADP-ribosylhydrolase 1 (hARH1, ADPRH) cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein. |
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