| Catalog No. | Size | Price (USD) |
| 204013-T42-50 | 50 µL | $147.96 |
| 204013-T42-100 | 100 µL | $251.64 |
| 204013-T42-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-DGCR8 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human DGCR8 |
| Immunogen | E. coli-derived Human DGCR8 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human DGCR8 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-C22orf12 Antibody; Anti-DGCRK6 Antibody; Anti-Gy1 Antibody; Anti-LP4941 Antibody; Anti-pasha Antibody
Image / Experimental Information
| Image 1 Description | Anti-DGCR8 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Human DGCR8 Western blot (WB) 27606 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-DGCR8 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa (We are unsure as to the identity of these extra bands.) |
| Image 2 Alt Text | Human DGCR8 Immunohistochemistry(IHC) 27607 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-DGCR8 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa (We are unsure as to the identity of these extra bands.) |
| Image 3 Alt Text | Human DGCR8 Immunofluorescence(IF) 27608 |
| Image 3 URL | Source image |
Background Information
| Description | DiGeorge syndrome chromosomal region 8 (DGCR8), a double-stranded-RNA-binding protein, participates in the miRNA biogenesis pathway and contributes to miRNA maturation by interacting with the RNAase III enzyme Drosha in cell nuclei. DGCR8, which is known as Pasha in flies and worms, is a 773-amino-acid (aa) protein critical for microRNA (miRNA) biogenesis via the formation of a microprocessor complex with Drosha. This complex processes primary miRNA in the nucleus. DGCR8 deletion in murine B-cell progenitors impairs the transition from pro- to early pre-B cell stage, recapitulating the phenotypes observed in miR17-92 cluster knockout mice. The DGCR8-mediated miRNA pathway is required for maintaining blood pressure, vascular reactivity and vascular wall remodeling at the postnatal stages. |
| References |
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