| Catalog No. | Size | Price (USD) |
| 13498-MM01T-50 | 50 µL | $147.96 |
| 13498-MM01T-100 | 100 µL | $251.64 |
| 13498-MM01T-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Doublecortin/DCX Antibody, Mouse Monoclonal |
| Validated applications | ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Doublecortin/DCX |
| Immunogen | Recombinant Human Doublecortin/DCX Protein (Catalog#13498-H09E) |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Source | Monoclonal Mouse IgG2a Clone #01 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, 0.02% Tween 80, pH7.0 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-DBCN Antibody; Anti-DC Antibody; Anti-LISX Antibody; Anti-SCLH Antibody; Anti-XLIS Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human DCX in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human DCX monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
| Image 1 Alt Text | Human DCX Immunofluorescence(IF) 2946 |
| Image 1 URL | Source image |
Background Information
| Full Name | doublecortin |
| Description | DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked. |
| References |
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