IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix protein VP40 Protein (Catalog#40446-V07E)
Preparation
Produced in rabbits immunized with purified, recombinant EBOV Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt / GIN / 2014 / Kissidougou-C15) Matrix protein VP40 (Catalog#40446-V07E; AHX24648.1; Asn31-Lys326), and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
Anti-Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix protein VP40 rabbit polyclonal antibody at 1:5000 dilution Lane A: Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 Protein (10ng) Lane B: Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 Protein (10ng) Lane C: Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 (10ng) Lane D: Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP40 (10ng) Lane E: Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix protein VP40 Protein(10ng) Lane F: Human CLIC1(10ng) Secondary Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.
Image 1 Alt Text
EBOV EBOV-VP40/Matrix protein VP40 Western blot (WB) 6757